Summary
An effective storage procedure has been demonstrated for the mosquito pathogen Culicinomyces clavisporus. A mycelial preparation was harvested by filtration, sprayed with a sucrose solution and air dried at 20°C in a laminar flow cabinet until the mycelial mat became crisp. This material was then ground in a hammer mill and particles of less than 355 μm were sieved out. Viability of the particles was assessed by studying mycelial growth and conidial formation when particles were added to water agar plates or to water. The particles retained 100% viability after 9 weeks storage at-20°C or 6 days at 4°C. Preparations lost activity rapidly if stored at 20°C. Conidia produced by this method were pathogenic to mosquito larvae.
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References
Frances SP, Lee DJ, Russell RC, Panter C (1985) Seasonal occurrence of the mosquito pathogenic fungus Culicinomyces clavisporus in a natural habitat. J Aust Ent Soc 24:241–246
Federici BA (1981) Mosquito Control by the fungi Culicinomyces, Lagenidium and Coelomomyces. In: Burges HD (ed) Microbial control of pests and plant diseases 1970–1980. Academic Press, pp 555–572
McCabe B, Soper RS (1985) Preparation of an entomopathogenic fungal insect control agent US Patent no 4530834
Sweeney AW (1981) The effects of low temperature storage on the infectivity of Culicinomyces conidia for mosquito larvae. J Invert Pathol 38:294–296
Sweeney AW (1985) The potential of the fungus Culicinomyces clavisporus as a biocontrol agent for medically important Diptera. In: Laird M, Miles JW (eds) Integrated Mosquito Control Methodologies. Vol 2. Academic Press, pp 264–284
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Roberts, D.W., Dunn, H.M., Ramsay, G. et al. A procedure for preservation of the mosquito pathogen Culicinomyces clavisporus . Appl Microbiol Biotechnol 26, 186–188 (1987). https://doi.org/10.1007/BF00253906
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DOI: https://doi.org/10.1007/BF00253906