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Chitinolytic properties of Streptomyces lividans

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Abstract

Streptomyces lividans TK24, an established host for genetic and molecular studies in actinomycetes, is able to use chitin as sole carbon and nitrogen source. Extracellular chitinase and N-acetyl-β-d-glucosamidinase (chitobiase) activities were detected in liquid cultures. Chitinase production was inducible by chitin and its low molecular weight derivatives. Low levels of chitinase were also produced in the absence of chitin. Production of extracellular N-acetylglucosaminidase was correlated with the beginning of the stationary phase of growth and was independent of the presence of chitin. Beside highly N-acetylated chitin, supernatants of chitin-induced cultures were able to hydrolyse chitosans with a wide range of degrees of N-acetylation.

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Abbreviations

MS:

minimal salts

GlcNAc:

N-acetyl-β-d-glucosamine

pNP-GlcNAc:

p-nitrophenyl-2-acetamido-2-deoxy-β-d-glucopyranoside

d.a.:

degree of N-acetylation

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Département de Biologie, Faculté des Sciences, Université de Sherbrooke, 2500 Bld Université, J1K 2R1, Sherbrooke, Québec, Canada

    Ewa Neugebauer, Benoit Gamache, Claude V. Déry & Ryszard Brzezinski

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  1. Ewa Neugebauer
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  2. Benoit Gamache
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  3. Claude V. Déry
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  4. Ryszard Brzezinski
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Neugebauer, E., Gamache, B., Déry, C.V. et al. Chitinolytic properties of Streptomyces lividans . Arch. Microbiol. 156, 192–197 (1991). https://doi.org/10.1007/BF00249114

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  • DOI: https://doi.org/10.1007/BF00249114

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