Abstract
The effect of copper supplementation on growth, methane monooxygenase activity and lipid composition of Methylococcus capsulatus (Bath) was studied. Copper increased biomass yield, methane monooxygenase activity and phospholipid content from 7.7 to 10.2% of dry weight. Cells from copper-deficient and copper supplemented cultures contained the same major fatty acids but in the presence of copper only the contents of C16:0 and the three C16:1 isomers were increased while the contents of C14:0 and cyclic C17:0 remained unchanged. Phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol and cardiolipin were analysed amongst the polar lipids. PE was the main component (about 60 mol-%) but the most notable copper-induced increment occurred in the proportion of PC, from about 10 to 16 mol-%. Concomitantly with this increment the fatty acids of PC were changed so that the mol-% of C16: 1 isomers were increased at the expense of other acids. Similar trends were seen also in the fatty acid compositions of other polar lipid fractions. It is therefore concluded that phosphatidylcholine would be one of the key factors when the role of membranous lipids in methane monooxygenase activity is to be considered.
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Anthony C (1987) The biochemistry of methane and methanol utilization. In: Stowell JD, Beardsmore AJ, Keevil CW, Woodward JR (eds) Carbon substrates in biotechnology, vol 21. IRL Press, Oxford, pp 93–118
Burrows KJ, Cornish A, Scott D, Higgins IJ (1984) Substrate specificities of the soluble and particulate methane mono-oxygenase of Methylosinus trichosporium OB3b. J Gen Microbiol 130: 3327–3333
Colby J, Stirling DI, Dalton H (1977) The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath), its ability to oxygenate n-alkanes, n-alkenes ethers and alicyclic, aromatic and heterocyclic compounds. Biochem J 65: 395–402
Collins LP, Buchholz LA, Remsen CC (1991) Effect of copper on Methylomonas albus BG8. Appl Environ Microbiol 57: 1261–1264
Cornish A, Mac Donald J, Burrows KJ, King TS, Scott D, Higgins IJ (1985) Succinate as an in vitro electron donor for the particulate methane mono-oxygenase of Methylosinus trichosporium OB3b. Biotechnol Lett 7: 319–324
Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226: 497–509
Fulco AJ (1983) Fatty acid metabolism in bacteria. Prog Lipid Res 22: 133–160
Guckert JB, Ringelberg DB, White DC, Hanson RS, Bratina BJ (1991) Membrane fatty acids as phenotypic markers in the polyphasic taxonomy of methylotrophs within the Proteobacteria. J Gen Microbiol 137: 2631–2641
Hagen P-O, Goldfine H, Williams PJL (1966) Phospholipids of bacteria with extensive intracytoplasmic membranes. Science 151: 1543–1544
Hyder SL, Meyers A, Cayer ML (1979) Membrane modulation in a methylotrophic bacterium Methylococcus capsulatus (Texas) as a function of growth substrate. Tissue Cell 11: 597–610
Jahnke LL, Nichols PD (1986) Methyl sterol and cyclopropane fatty acid composition of Methylococcus capsulatus grown at low oxygen tensions. J Bacteriol 167: 238–242
Leak DJ, Dalton H (1986a) Growth yield of methanotrophs. 1. Effect of copper on the energetics of methane oxidation. Appl Microbiol Biotechnol 23: 470–476
Leak DJ, Dalton H (1986b) Growth yield of methanotrophs. 2. A theoretical analysis. Appl Microbiol Biotechnol 23: 477–481
Leak DJ, Stanley SH, Dalton H (1985) Implications of the nature of methane monooxygenase on carbon assimilation in methanotrophs. In: Poole RK, Dow CS (eds) Microbial gas metabolism, mechanistic, metabolic and biotechnological aspects. Academic Press, London, pp 201–208
Makula RA (1978) Phospholipid composition of methane-utilizing bacteria. J Bacteriol 134: 771–777
Patt TE, Hanson RS (1978) Intracytoplasmic membrane, phospholipid, and sterol content of Methylobacterium organophilum cells grown under different conditions. J Bacteriol 134: 636–644
Prior SD, Dalton H (1985) The effect of copper ions on membrane content and methane monooxygenase activity in methanol-grown cells of Methylococcus capsulatus (Bath). J Gen Microbiol 131: 155–163
Scott D, Brannan J, Higgins IJ (1981) The effect of growth conditions on intracytoplasmic membranes and methane mono-oxygenase activities in Methylosinus trichosporium OB3b. J Gen Microbiol 125: 63–72
Smith DDS, Dalton H (1989) Solubilisation of methane monooxygenase from Methylococcus capsulatus (Bath). Eur J Biochem 182: 667–671
Smith U, Ribbons DW, Smith DS (1970) The fine structure of Methylococcus capsulatus. Tissue Cell 2: 513–520
Stanley SH, Prior SD, Leak DJ, Dalton H (1983) Copper stress underlies the fundamental change in intracellular location of methane mono-oxygenase in methane-oxidizing organisms: studies in batch and continuous cultures. Biotechnol Lett 5: 487–492
Suutari M, Liukkonen K, Laakso S (1990) Temperature adaptation in yeasts: the role of fatty acids. J Gen Microbiol 136: 1469–1474
Takeda K, Tanaka K (1980) Ultrastructure of intracytoplasmic membranes of Methanomonas margaritae cells grown under different conditions. Antonie van Leeuwenhoek 46: 15–25
Takeda K, Tezuka C, Fukuoka S, Takahara Y (1976) Role of copper ions in methane oxidation by Methanomonas margaritae. J Ferment Technol 54: 557–562
Whittenbury R, Phillips KC, Wilkinson JF (1970) Enrichment, isolation and some properties of methane-utilizing bacteria. J Gen Microbiol 61: 205–218
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Peltola, P., Priha, P. & Laakso, S. Effect of copper on membrane lipids and on methane monooxygenase activity of Methylococcus capsulatus (Bath). Arch. Microbiol. 159, 521–525 (1993). https://doi.org/10.1007/BF00249029
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DOI: https://doi.org/10.1007/BF00249029