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Respiratory activity in the marine cyanobacterium Spirulina subsalsa and its role in salt tolerance

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Abstract

Intracellular ion concentration and respiratory activity in the marine cyanobacterium Spirulina subsalsa was analyzed during cell transition from saline to hypersaline medium. During salt upshock, an early phase of Na+ and Cl- influx was observed, followed by an adaptation phase where both Na+ and Cl- were excluded from the cell. Respiration in intact cells was enhanced during salt upshock. S. subsalsa spheroplasts exhibited a high rate of O2 uptake, which was further enhanced in cells grown in hypersaline medium, upon addition of NaCl to the assay mixture. This effect was found to be specific to sodium ions. Plasma membrane fractions from cells grown in hypersaline medium exhibited a high rate of cytochrome oxidase activity, which was further stimulated by NaCl, and was sensitive to DCCD. Immunoblot analysis of Spirulina plasma membrane polypeptides with anti-cytochrome oxidase serum demonstrated high content of 53.4 kDa polypeptide of cytochrome oxidase, which was enriched in membranes obtained from hypersaline Spirulina cells. The enhanced respiration, and more specifically the enrichment of cytochrome oxidase activity in salt-adapted cells in situ, as well as its stimulation by NaCl in vitro and inhibition by DCCD, suggest that cytochrome oxidase is involved in the extrusion of sodium ions from cells of the salt-tolerant Spirulina subsalsa.

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Abbreviations

DCCD:

dicyclohexylcarbodiimide

CCCP:

carbonylcyanide m-chlorophenyl hydrazone

TMPD:

N, N, N′, N′, tetramethyl p-phenylenediamine dichloride

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Authors and Affiliations

  1. Department of Agricultural Botany, The Hebrew University of Jerusalem, P.O. Box 12, 76100, Rehovot, Israel

    Rachel Gabbay-Azaria, Mordechay Schonfeld & Elisha Tel-Or

  2. Department of Organic Chemistry, The Weizmann Institute of Science, 76100, Rehovot, Israel

    Shoshana Tel-Or

  3. Department of Field-Crops, The Hebrew University of Jerusalem, 76100, Rehovot, Israel

    Rachel Messinger

Authors
  1. Rachel Gabbay-Azaria
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  2. Mordechay Schonfeld
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  3. Shoshana Tel-Or
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  4. Rachel Messinger
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  5. Elisha Tel-Or
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Gabbay-Azaria, R., Schonfeld, M., Tel-Or, S. et al. Respiratory activity in the marine cyanobacterium Spirulina subsalsa and its role in salt tolerance. Arch. Microbiol. 157, 183–190 (1992). https://doi.org/10.1007/BF00245288

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  • DOI: https://doi.org/10.1007/BF00245288

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