Skip to main content
SpringerLink
Log in

Effects of light on protein secretion in Neurospora crassa

  • Original Papers
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

The relative concentrations of secreted proteins in liquid cultures of Neurospora crassa differ in constant darkness compared to constant light (2500lx). Light reduces the concentrations of some polypeptides markedly and increases the concentrations of protein species of 67, 40, 18 and 13 kDa. The “blind” wc-2 mutant of Neurospora does not show light dependent differences in amounts of secreted proteins. One of the light-sensitive extracellular proteins is shown to be a protease of 17,5 kDa.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chambers JAA, Hinkelammert K, Russo VEA (1985) Lightregulated protein and poly-(A+)-mRNA synthesis in Neurospora crassa. EMBO J 13: 3649–3653

    Article  Google Scholar 

  • Cohen BL, Drucker H (1977) Regulation of exocellular protease in Neurospora crassa: induction and repression under conditions of nitrogen starvation. Arch Biochem Biophys 182: 601–613

    Article  CAS  Google Scholar 

  • Fiema J (1979) The hydroxyproline content in the mycelium of Aspergillus giganteus mut. alba. Bull Acad Pol Sci 27: 121–124

    CAS  Google Scholar 

  • Furukawa K, Hasunuma K, Shinohara Y (1987) Characterization of Pi-repressible enzymes secreted in culture media by Neurospora crassa wild-type cells and null-type mutants. J of Bacteriol 169: 4790–4795

    Article  CAS  Google Scholar 

  • Graafmans WDJ (1977) Effect of blue light on metabolism in Penicillium isariiforme. J Gen Microbiol 101: 157–161

    Article  CAS  Google Scholar 

  • Gressel J (1980) Blue light and transcription, In: Senger H (ed) The blue light syndrome. Springer, Berlin Heidelberg New York, pp 133–153

    Chapter  Google Scholar 

  • Gressel J, Rau W (1983) Photocontrol of fungal development, In: Shropshire W, Mohr H (eds) Photomorphogenesis. Encyclopedia of plant physiology 16b. Springer, Berlin Heidelberg New York, pp 603–639

    Google Scholar 

  • Hanson MA, Marzluf GA (1973) Regulation of a sulfur-controlled protease in Neurospora crassa. J Bacteriol 116: 785–789

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hashimoto J, Uchida T, Egami F (1971) Purification of ribonuclease U1 and some properties of ribonucleases U1 and N1. J Biochem 78: 903–911

    Article  Google Scholar 

  • Hasunuma K (1973) Repressible extracellular nucleases in Neurospora crassa. Biochim Biophys Acta 319: 288–293

    Article  CAS  Google Scholar 

  • Hasunuma K (1983) Repressible extracellular phosphodiesterases showing cyclic 2′,3′- and cyclic 3′,5′-nucleotide phosphodiesterase activities in Neurospora crassa. J Bacteriol 156: 291–300

    CAS  PubMed  PubMed Central  Google Scholar 

  • Heussen C, Dowdle EB (1980) Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing dodecyl sulfate and copolymerized substrates. Anal Biochem 102: 196–202

    Article  CAS  Google Scholar 

  • Horowitz NH (1947) Methionine synthesis in Neurospora. J Biol Chem 171: 255–264

    CAS  Google Scholar 

  • Laemmli HK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. nature 227: 680–685

    Article  CAS  Google Scholar 

  • Lindberg RA, Eirich LD, Price JS, Wolfinbarger L, Drucker H (1981) Alkaline protease from Neurospora crassa. J Biol Chem 256: 811–814

    CAS  PubMed  Google Scholar 

  • Lindberg RA, Rhodes WG, Eirich LD, Drucker H (1982) Extracellular acid proteases from Neurospora crassa. J Bacteriol 150: 1103–1108

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the phenol reagent. J Biol Chem 139: 265–275

    Google Scholar 

  • Metzenberg RL (1979) Implications of some genetic control mechanisms in Neurospora. Microbiol Rev 43: 361–383

    CAS  PubMed  PubMed Central  Google Scholar 

  • Nahas E, Terenzi HF, Rossi A (1982) Effect of carbon source and pH on the production and secretion of acid protease (EC 3.1.3.2) and alkaline phosphatase (EC 3.1.3.1) in Neurospora crassa. J Gen Microbiol 128: 2017–2021

    CAS  Google Scholar 

  • Neuhoff V, Stamm R, Eibl HJ (1985) Intensive Proteinfärbung ohne Hintergrund mit Coomassie Blau G-250 in Polyacrylamidgelen: Theorie und Praxis. Electrophoresis 6: 87–91 and 330–334

    Article  Google Scholar 

  • Piskorz-Bincycka B (1978) Studies on the organic acids content of the mycelia of Penicillium isariiforme. Acta Biol Cracov: 21–22

  • Richter G (1984) Blue light effects on the level of translation and transcription. In: Senger H (ed) The blue light effects in biological systems. Springer, Berlin Heidelberg New York, pp 253–262

    Chapter  Google Scholar 

  • Russo VEA (1988) Blue light induces circadian rhythms in the bd mutant of Neurospora crassa: double mutants bd,wc-1 and bd,wc-2 are blind. J Photochem Photobiol 2: 59–65

    Article  CAS  Google Scholar 

  • Ruythers G (1980) Effects of blue light on enzymes. In: Senger H (ed) The blue light syndrome. Springer, Berlin Heidelberg New York, pp 309–318

    Google Scholar 

  • Sommer T, Chambers JAA, Eberle J, Lauter FR, Russo VEA (1989) Fast light-regulated genes of Neurospora crassa. Nucleic Acids Res 14: 5713–5723

    Article  Google Scholar 

  • Tamaru H, Inoue H (1989) Isolation and characterization of a laccase-derepressed mutant of Neurospora crassa. J Bacteriol 171: 6288–6293

    Article  CAS  Google Scholar 

  • Turian G, Bianchi DE (1971) Conidiation in Neurospora crassa. Arch Mikrobiol 77: 262–274

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Zellbiologie, Biochemie und Biotechnologie der Universität, Bibliothekstraße, Postfach 33 04 40, W-2800, Bremen 33, Germany

    Andreas Kallies, Saadat Mohsenzadeh & Ludger Rensing

Authors
  1. Andreas Kallies
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Saadat Mohsenzadeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ludger Rensing
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kallies, A., Mohsenzadeh, S. & Rensing, L. Effects of light on protein secretion in Neurospora crassa . Arch. Microbiol. 157, 104–106 (1992). https://doi.org/10.1007/BF00245276

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00245276

Key words

Search

Navigation