β-Oxidation enzymes were detected both in the mitochondria and microbodies of Arum maculatum L. spadices and Brassica napus L. seeds. It is apparent that the mitochondrial membrane barrier, which remains intact after sucrose-density-gradient centrifugation, prevents rapid access of acyl-GoA substrates to matrix βoxidation tes. Thus intact mitochondria showed little β-oxidation enzyme activity. Rupturing of the mitochondrial membrane allowed rapid access of acyl CoAs to matrix sites. Consequently, in ruptured mitochondria, high β-oxidation enzyme activities were measured.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Aebi, H. (1974) Catalase. In: Methods of enzymatic analysis, vol. 2, pp. 673–684, Bergmeyer, H.E., ed. Verlag Chemie, Weinheim
ap Rees, T. (1980) Assessment of the contributions of metabolic pathways to plant respiration. In: The biochemistry of plants, a comprehensive treatise, vol. 2. pp. 1–29, Stumpf, P.K., Conn, E.E., eds. Academic Press, New York
Beevers, H. (1979) Microbodies in higher plants. Annu. Rev. Plant Physiol. 30, 159–193
Beevers, H., Breidenbach, R.W. (1974) Glyoxysomes. Methods Enzymol. 31, 565–571
Bieber, L.L., Krahling, J.B., Clarke, P.R.H., Valkner, K.J., Tolbert, N.E. (1981) Carnitine acyltransferases in rat liver peroxisomes. Arch. Biochem. Biophys. 211, 599–604
Burgess, N., Thomas, D.R. (1986) Carnitine acetyltransferase in pea cotyledon mitochondria. Planta. 167, 58–65
Burgess, N., Beakes, G.W., Thomas, D.R. (1985) Separation of mitochondria from microbodies of Pisum sativum (L. cv. Alaska) cotyledons. Planta 166, 151–155
Chapman, D., Leslie, R.B. (1970) Structure and function of phospholipids in membranes. In: Membranes of mitochondria and chloroplasts, pp. 91–115, Racker, E., ed. Van Nostrand Reinold Company, New York London
Cooper, T.G., Beevers, H. (1969a) Mitochondria and glyoxysomes from castor bean endosperm. Enzyme constituents and catalytic capacity. J. Biol. Chem. 244, 3507–3513
Cooper, T.G., Beevers, H. (1969b) β-oxidation in glyoxysomes from castor bean endosperm. J. Biol. Chem. 244, 3514–3520
Donaldson, R.P., Tully, R.E., Young, O.A., Beevers, H. (1981) Organelle membranes from germinating castor bean endosperm. II. Enzymes, cytochromes and permeability of the glyoxysome membrane. Plant Physiol. 67, 21–25
Feierabend, J., Beevers, H. (1972) Developmental studies on microbodies in wheat leaves. 1. Conditions influencing enzyme development. Plant Physiol. 49, 28–32
Galliard, T. (1980) Degradation of acyl lipids: hydrolytic and oxidative enzymes. In: The biochemistry of plants, a comprehenive treatise, vol. 4, pp. 85–116, Stumpf, P.K., Conn, E.E., eds. Academic Press, New York
Gerbling, H., Gerhardt, B. (1988) Carnitine acyltransferase activity of mitochondria from mung bean hypocotyls. Planta 174, 90–93
Gerhadt, B., ed. (1978) Microbodies/Peroxisomen pflanzlicher Zellen. (Cell Biology Monographs, vol. 5). Springer, Vienna New York
Gerhardt, B. (1981) Enzyme activities of the β-oxidation pathway in spinach leaf peroxisomes. FEBS Lett. 126, 71–73
Gerhardt, B. (1983) Localization of β-oxidation enzymes in peroxsomes isolated from non-fatty plant tissues. Planta 159, 238–246
Hill, R.L., Bradshaw, R.A. (1969) Fumarase. Methods Enzymol. 13, 91–99
Hutton, D., Stumpf, P.K. (1969) Fat metabolism in higher plants. XXXVII. Characterization of the β-oxidation systems from maturing and germinating castor bean seeds. Plant Physiol. 44, 508–516
Kindl, H. (1987) β-Oxidation of fatty acids by specific organelles. In: The biochemistry of plants, a comprehensive treatise, vol. 9, pp. 31–52, Stumpf, P.K., Conn, E.E., eds. Academic Press, New York
Macey, M.J.K. (1983) β-oxidation and associated enzyme activities in microbodies from germinating peas. Plant Sci. Lett. 30, 53–60
Macey, M.J.K., Stumpf, P.K. (1983) β-oxidation enzymes in microodies from tubers of Helianthus tuberosus. Plant Sci. Lett. 28, 207–212
Markwell, M.A.K., Haas, S.M., Bieber, L.L., Tolbert, N.E. (1978) A modification of the Lowry procedure to simplify protein determination in membrane and lipo-protein samples. Anal. Biochem. 87, 206–210
Mazliak, P. (1973) Lipid metabolism in plants. Annu. Rev. Plant Physiol. 24, 287–310
McNeil, P.H., Thomas, D.R. (1976) The effect of carnitine on palmitate oxidation by pea cotyledon mitochondria. J. Exp. Bot. 27, 1163–1180
Miernyk, J.A., Trelease, R.N. (1981) Control of enzyme activities in cotton cotyledons during maturation and germination. IV. β-oxidation. Plant Physiol. 67, 341–346
Nishimura, T., Saito, T., Tomita, K. (1978) Purification and properties of β-Ketothiolase from Zoogloea ramigera. Arch. Microbiol. 116, 21–27
Olsen, J.A., Huang, A.H.C. (1988) Glyoxysomal acyl CoA synthetase and oxidase from germinating elm, rape and maize seed. Phytochemistry 27, 1601–1603
Overath, P., Raufuss, E.M., Stoffel, M., Ecker, W. (1967) The induction of enzymes of fatty acid degradation in Escherichia coli. Biochem. Biophys. Res. Commun. 29, 28–33
Panter, R.A., Mudd, J.B. (1969) Carnitine levels in some higher plants. FEBS Lett. 5, 169–170
Panter, R.A., Mudd, J.B. (1973) Some aspects of carnitine metaboism in avocado (Persea americana). Biochem. J. 134, 655–658
Thomas, D.R., McNeil, P.H. (1976) The effect of carnitine on the oxidation of saturated fatty acids by pea cotyledon mitochondria. Planta 132, 61–63
Thomas, D.R., Wood, C. (1986) The two β-oxidation sites in pea cotyledons. Carnitine palmitoyltransferase: location and function in pea mitochondria. Planta 168, 261–266
Thomas, D.R., Noh Hj Mil, M., Cooke, R.J., Yong, B.C.S., Ariffin, A., McNeil P.H., Wood, C. (1982) The synthesis of palmitoylcarnitine by etio-chloroplasts of greening barley leaves. Planta 154, 60–65
Tolbert, N.B. (1981) Metabolic pathways in peroxisomes and glyoxysomes. Annu. Rev. Biochem. 50, 133–157
Walker, D.B., Gysi, J., Sternberg, L., De Niro, M.J. (1983) Direct respiration of lipids during heat production in the inflorescence of Philodendron selloum. Science 220, 419–421
Wood, C., Noh Hj Jalil, M., Ariffin, A., Yong, B.C.S., Thomas, D.R. (1983) Carnitine short-chain acyltransferase in pea mitochondria. Planta 158, 175–178
Wood, C., Noh Hj Jalil, M., McLaren, I., Yong, B.C.S., Ariffin, A., McNeil, P.H., Burgess, N., Thomas, D.R. (1984) Carnitine long-chain acyltransferase and oxidation of palmitate, palmitoyl coenzyme A and palmitoylcarnitine by pea mitochondria preparations. Planta 161, 255–260
Wood, C., Burgess, N., Thomas, D.R. (1986) The dual location of β-oxidation enzymes in germinating pea cotyledons. Planta 167, 54–57
C. Masterson thanks the Science and Engineering Research Council for the award of a postgraduate student maintenance grant. D.R. Thomas and C. Wood thank their relatives for continuing financial support. The authors also thank West Cumberland Farmers Ltd., Hexham, UK for their gift of oilseed rape seeds.
About this article
Cite this article
Masterson, C., Wood, C. & Thomas, D.R. β-Oxidation enzymes in the mitochondria of Arum and oilseed rape. Planta 182, 129–135 (1990). https://doi.org/10.1007/BF00239994
- Brassica (β-oxidation)
- (enzyme location)