Abstract
The effects of CoCl2, AgNO3 and ethylene released by exogenous 2-chloroethylphosphonic acid (Ethephon), were studied on shoot regeneration from cotyledons of Helianthus annuus cv. E8206R, a poorly regenerative cultivar. Inhibition of ethylene biosynthesis by CoCl2, at concentrations of 20 μK, provoked a substantial enhancement of shoot regeneration (30 %): the control was poorly regenerative. However, CoCl2 had no effect when Ethephon was supplied. Inhibition of ethylene action by AgNO3, at concentrations of 10–25 μM, caused a significant increase in plant regeneration: 25 % instead of 1.2 % in the control. Furthermore, addition of Ethephon to AgNO3-treated tissues failed to reduce the stimulation of shoot regeneration caused by AgNO3. On the basis of these findings, it is suggested that ethylene inhibits the regeneration process from cotyledons of sunflower.
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Abbreviations
- NAA:
-
1-naphthalene acetic acid
- BAP:
-
6-benzylamino-purine
- GA3 :
-
gibberellic acid
- Ethephon:
-
2-chloroethylphosphonic acid
- MS:
-
Murashige and Skoog medium
- AVG:
-
aminoethoxyvinylglycine
References
Beyer E.M. (1976) Plant Physiol. 58:268–271.
Beyer E.M. (1979) Plant Physiol. 63:169–173.
Chi G.L. and Pua E.C. (1989) Plant Science 64:243–250.
Curtis R.W. (1982) Plant Growth Regulation 1:119–138.
Espinasse A., Lay C. and Volin J. (1989) Plant Cell, Tissue and Organ Culture 17:171–181.
Finer J.J (1987) Plant Cell Reports 6:372–374.
Freyssinet M. and Freyssinet G. (1988) Plant Science 56:177–181.
Greco B., Tanzarella O.A., Carrozzo G. and Blanco A. (1984) Plant Science Letters 36:73–77.
Lau O.L. and Yang S.F. (1976) Plant Physiol. 58:114–117.
Lieberman M. (1979) Annu. Rev. Plant Physiol. 30:533–591.
Lupi M.C., Bennici A., Locci F. and Gennai D. (1987) Plant Cell, Tissue and Organ Culture 11:47–55.
Murashige T. and Skoog F. (1962) Physiol. Plant. 15:473–493.
Paterson K.E. and Everett N.P. (1985) Plant Science 42:125–132.
Paterson-Robinson K.E. and Adams D.O. (1987) Physiol. Plant. 71:151–156.
Pélissier B., Bouchefra O., Pépin R. and Freyssinet G. (1990) Plant Cell Reports 9:47–50.
Power C.J. (1987) Amer. J. Bot. 74:497–503.
Prado E. and Bervillé A. (1990) Plant Science 67:73–82.
Purnhauser L., Medgyesy P., Czako M., Dix P.J. and Marton L. (1987) Plant Cell Reports 6:1–4.
Purvis A.C. (1980) Plant Physiol. 66:624–627.
Roustan J.P., Latché A. and Fallot J. (1989) Plant Cell Reports 8:182–185.
Roustan J.P., Latché A. and Fallot J. (1990) Plant Science 67:89–95.
Songstad D.D., Duncan D.R. and Widholm J.M. (1988) Plant Cell Reports 7:262–265.
Vain P., Yean H. and Flament P. (1989) Plant Cell, Tissue and Organ Culture 18:143–151.
Veen H. (1979) Planta 145:467–470.
Wilcox McCann A., Cooley G. and Van Dreser J. (1988) Plant Cell, Tissue and Organ Culture 14:103–110.
Witrzens B., Scowcroft W.R., Downes R.W. and Larkin P.J. (1988) Plant Cell, Tissue and Organ Culture 13:61–76.
Yang S.F. and Hoffman N.E. (1984) Annu. Rev. Plant Physiol. 35:155–189.
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Communicated by A. M. Boudet
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B., K.M.C., Latche, A., Roustan, JP. et al. Stimulation of shoot regeneration from cotyledons of Helianthus annuus by the ethylene inhibitors, silver and cobalt. Plant Cell Reports 10, 204–207 (1991). https://doi.org/10.1007/BF00234296
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DOI: https://doi.org/10.1007/BF00234296