Summary
The incidence of myosatellite cells associated with “white” and “red” muscle fibres of the parietal muscle and “red” fibres of the craniovelar muscle was estimated by quantitative electron microscopy in the Atlantic hagfish (Myxine glutinosa, L.). Myosatellite cell nuclei constitute 3, 11 and 23 % of the total number of nuclei inside the basal lamina of the three types of muscle fibres, respectively. However, the total number of nuclei is highest in “white” fibres, most of the nuclei belonging to striated muscle cells. Myosatellite cell profiles in transverse sections constitute 23, 41 and 61 % of the number of muscle fibre profiles of the three types, respectively. The intervals between adjacent myosatellite cells are ∼ 135 μm in “white” fibres, ∼ 55 μm in “red” parietal fibres, and only ∼ 25 μm in craniovelar fibres. Since craniovelar fibres are also comparatively thin, myosatellite cells constitute a significant fraction of the volume inside the basal lamina in these fibres. The myosatellite cells are ∼ 30–50 μm long and up to 5 μm thick. Some myosatellite cells possess few organelles, whereas others appear to contain many free ribosomes, granular endoplasmic reticulum, prominent Golgi apparatus and lysosome-like bodies.
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References
Abercrombie, M.: Estimation of nuclear population from microtome sections. Anat. Rec. 94, 239–247 (1941)
Allbrook, D.B., Han, M.F., Hellmuth, A.E.: Population of muscle satellite cells in relation to age and mitotic activity. Pathology 3, 233–243 (1971)
Andersen, P., Jansen, J.K.S., Løyning, Y.: Slow and fast muscle fibres in the Atlantic hagfish (Myxine glutinosa). Acta physiol. scand. 57, 167–179 (1963)
Bone, Q.: On the function of the two types of myotomal muscle fibre in elasmobranch fish. J. Mar. biol. Ass. U.K. 46, 321–349 (1966)
Church, J.C.T.: Satellite cells and myogenesis; a study in the fruit-bat web. J. Anat. (Lond.) 105, 419–438 (1969)
Church, J.C.T.: Cell populations in skeletal muscle after regeneration. J. Embryol. exp. Morph. 23, 531–537 (1970)
Davison, W., Goldspink, G., Johnston, I.A.: Division of labour between fish myotomal muscles during swimming. J. Physiol. (Lond.) 263, 185P-186P (1976)
Flood, P.R.: Myosatellite cells in Myxine and axolotl. In: Proc. 3rd Eur. Conf. on Elect. Micr., pp. 575–576 (1964)
Flood, P.R.: The three dimensional structure and frequency of myosatellite cells in trunk muscles of the axolotl (Siredon mexicanus). J. Ultrastruct. Res. 36, 523–524 (1971)
Hanzliková, V., Macková, E.V., Hnik, P.: Satellite cells of the rat soleus muscle in the process of compensatory hypertrophy combined with denervation. Cell Tiss. Res. 160, 411–421 (1975)
Hellmuth, A.E., Allbrook, D.: Satellite cells as stem cells of skeletal muscle. Excerpta Medica Int. Congr. Ser. 294, 343–345 (1973)
Hess, A., Rosner, S.: The satellite cell bud and myoblast in denervated mammalian muscle fibers. Amer. J. Anat. 129, 21–40 (1970)
Kelly, A.M.: Satellite cells in the soleus and extensor digitorum longus muscles of rats. J. Cell Biol. 67, 206a (1975)
Korneliussen, H.: Identification of muscle fiber types in “semithin” sections stained with p-phenylene-diamine. Histochemie 32, 95–98 (1972)
Korneliussen, H., Nicolaysen, K.: Ultrastructure of four types of striated muscle fibers in the Atlantic hagfish (Myxine glutinosa, L.). Z. Zellforsch. 143, 273–290 (1973)
Korneliussen, H., Nicolaysen, K.: Distribution and dimension of the T-system in different muscle fiber types in the Atlantic hagfish (Myxine glutinosa, L.). Cell Tiss. Res. 157, 1–16 (1975)
Kryvi, H.: The structure of the myosatellite cells in axial muscles of the shark (Galeus melastomus). Anat. Embryol. 147, 35–44 (1975)
Kryvi, H., Eide, A.: Morphometric and autoradiographic studies on the growth of red and white axial muscle fibres in the shark Etmopterus spinax. Anat. Embryol. 151, 17–28 (1977)
Mauro, A.: Satellite cell of skeletal muscle fibers. J. biophys. biochem. Cytol. 9, 493–494 (1961)
Moss, F.P., Leblond, C.P.: Satellite cells as the source of nuclei in muscles of growing rats. Anat. Rec. 170, 421–436 (1971)
Nicolaysen, K.: On the functional properties of the fast and slow cranial muscles of the Atlantic hagfish. Acta physiol. scand. Suppl. 277, 68 (1966)
Noether, G.E.: Introduction to statistics. Boston: Houghton Mifflin Company 1971
Ontell, M.: Muscle satellite cells: A validated technique for light microscopic identification and a quantitative study of changes in their population following denervation. Anat. Rec. 178, 211–228 (1974)
Ontell, M.: Neonatal muscle: an electron microscopic study. Anat. Rec. 189, 669–690 (1977)
Reznik, M.: Origin of myoblasts during skeletal muscle regeneration. Electron microscopic observations. Lab. Invest. 20, 353–363 (1969)
Reznik, M.: Origin of the myogenic cell in the adult striated muscle of mammals. A review and a hypothesis. Differentiation 7, 65–73 (1976)
Schmalbruch, H., Hellhammer, U.: The number of satellite cells in normal human muscle. Anat. Rec. 185, 279–288 (1976)
Schmalbruch, H., Hellhammer, U.: The number of nuclei in adult rat muscles with special reference to satellite cells. Anat. Rec. 189, 169–176 (1977)
Schultz, E.: A quantitative study of satellite cell population in postnatal mouse lumbrical muscle. Anat. Rec. 180, 589–596 (1974)
Schultz, E.: Fine structure of satellite cells in growing skeletal muscle. Amer. J. Anat. 147, 49–70 (1976)
Snow, M.H.: Myogenic cell formation in regenerating rat skeletal muscle injured by mincing. I. A fine structural study. Anat. Rec. 188, 181–200 (1977a)
Snow, M.H.: Myogenetic cell formation in regenerating rat skeletal muscle injured by mincing. II. An autoradiographic study. Anat. Rec. 188, 201–218 (1977b)
Teräväinen, H.: Satellite cells of striated muscle after compression injury so slight as not to cause degeneration of the muscle fibers. Z. Zellforsch. 103, 320–327 (1970)
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This investigation was supported by the Norwegian Research Council for Science and the Humanities (NAVF grant No. C20.30–37). The authors are indebted to Jorunn Line Vaaland and Berit Branil for technical assistance, and to Dr. Finn Walvig, Biological Station, University of Oslo, Drøbak, for supplying the hagfish
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Sandset, P.M., Korneliussen, H. Myosatellite cells associated with different muscle fibre types in the Atlantic hagfish (Myxine glutinosa, L.). Cell Tissue Res. 195, 17–27 (1978). https://doi.org/10.1007/BF00233674
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DOI: https://doi.org/10.1007/BF00233674