Summary
We have evaluated the immunosuppressive effect of a new stable derivative of cyclophosphamide (ASTA Z 7557) on human lymphocyte immunoglobulin biosynthesis in vitro. When graded amounts of the drug are added to lymphocyte cultures stimulated with the helper T cell-dependent activator (PWM), a marked inhibition of B cell proliferation and differentiation occurs. Lymph node cells are particularly sensitive to the drug, while splenocytes are relatively resistant. The agent exerts only a minor effect on immunoglobulin synthesis triggered by a direct B cell stimulant (S. paratyphi bacteria).
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References
Gorski A, Korczak G, Wasik M, Nowaczyk M, Podobinska I, Malejczyk M, Gaciong Z, Paczek L: Mode of action of cyclophosphamide as assessed by in vitro activity of its active metabolite. Transplantation Proc 15:573, 1983
Gorski A, Nowaczyk M, Rowinski W: Immunomodulatory influence of active metabolite of cyclophosphamide on human lymphocyte immunoglobulin biosynthesis in vitro. Transplantation 35:577, 1983
Gorski A, Korczak-Kowalska G, Chorzelski T: Intracellular localization of synthesized immunoglobulins in B cells: a modified method. J Immunol Methods 57:33, 1983
Pohl J, Hilgard P: Experimental toxicology of ASTA Z 7557. Invest N Drugs 2:2, 1984
Bird AG, McLachlan SM, Britton S: Cyclosporin A promotes spontaneous outgrowth in vitro of Epstein-Barr virus-induced B-cell lines. Nature 289:300, 1981
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Gorski, A., Korczak-Kowalska, G. Inhibition of human B lymphocyte differentiation by a stable metabolite of cyclophosphamide (ASTA Z 7557, INN mafosfamide). Invest New Drugs 2, 227–229 (1984). https://doi.org/10.1007/BF00232356
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DOI: https://doi.org/10.1007/BF00232356