Summary
To clarify the structural base of immune response occurring in the kidney, we investigated the antigenic and functional properties of vascular endothelial cells. Peritubular capillary endothelial cells exhibited the same immuno-histochemical characteristics (OKM5-positive, HLA-DR-positive, Factor VIII/von Willebrand factor antigen-negative, Interleukin 1-positive) as a peripheral blood macrophage subset capable of presenting soluble antigens and triggering the autologous mixed lymphocyte reaction. On the other hand, endothelial cells of glomerular capillary loops, considered to be involved in blood coagulation, were OKM5-negative, HLA-DR-positive, Factor VIII/von Willebrand factor antigen-positive, Interleukin 1-positive. Thus the results of this study suggest that vascular endothelial cells in different anatomic compartments of the kidney express surface antigens heterogenously and may play different roles in the immune reaction.
Similar content being viewed by others
References
Anderson ND, Wyllie RG, Shaker JI (1977) Pathogenesis of vascular injury in rejecting rat renal allografts. Johns Hopkins Med 1141:135–147(1977)
Baldwin III WM, Soulillou J-P, Claas FHJ, Peyrat M-A, Es LA van, Rood JJ van (1981) Antibodies to endothelial antigens in eluates of 88 human kidneys. Correlation with graft survival and presence of T- and B-cell antibodies. Transplant Proc 13:1547–1550
Barnwell JW, Ockenhouse CF, Knowles II DM (1985) Monoclonal antibody OKM5 inhibits the in vitro binding of Plasmodium falciparum-infected erythrocytes to monocytes, endothelial, and C32 melanoma cells. J Immunol 135:3494–3497
Bloom AL, Gidding JC, Wilks CJ (1973) Factor VIII on the vascular intima: Possible importance in haemostasis and thrombosis. Nature (New Biol) 241:217–219
Bread J, Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1980) A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol 124:1943–1948
Castellot JJ, Hoover RL, Karnovsky MJ (1986) Glomerular endothelial cells secrete a heparinlike inhibitor and a peptide stimulater of mesangial cell proliferation. Am J Pathol 125:493–500
Dunnill MS (1984) Histopathology of rejection in renal transplantation. In: Morris PJ (ed) Kidney transplantation. Grune and Stratton, London, pp 355–380
Gurner AC, Smith J, Cattell V (1987) The origin of Ia antigenexpressing cells in the rat kidney. Am J Pathol 127:342–348
Hart DNJ, Fuggle SV, Williams KA, Fabre JW, Ting A, Morris PJ (1981) Localization of HLA-ABC and DR antigens in human kidney. Transplantation 31:428–433
Henny FC, Weening JJ, Baldwin WM, Oljans PJ, Tanke HJ, van Es LA, Paul LC (1986) Expression of HLA-DR antigens on peripheral blood T lymphocytes and renal graft tubular epithelial cells in association with rejection. Transplantation 42:479–483
Hirschberg H, Evenson SA, Henriksen T, Thorsby E (1975) The human mixed lymphocyte-endothelium culture interaction. Transplantation 19:495–504
Hirschberg H, Moen T, Thorsby E (1979) Specific destruction of human endothelial cell monolayers by anti-DRw antisera. Transplantation 28:116–120
Hirschberg H, Bergh OJ, Thorsby E (1980) Antigen-presenting properties of human vascular endothelial cells. J Exp Med 152:249s-255s
Hoyer LW, de los Santos RP, Hoyer JR (1973) Antihemophilic factor antigen. Localization in endothelial cells by immunofluorescent microscopy. J Clin Invest 52:2737–2744
Hoyer JR, Michael AF, Hoyer LW (1974) Immunofluorescent localization of antihemophilic factor antigen and fibrinogen in human renal diseases. J Clin Invest 53:1375–1384
Knowles II DM, Tolidjian B, Marboe C, D'agati V, Grimes M, Chess L (1984) Monoclonal anti-human monocyte antibodies OKM1 and OKM5 possess distinctive tissue distributions including differential reactivity with vascular endothelium. J Immunol 132:2170–2173
McLean IW, Nakane PK (1974) Periodate-lysine-paraformalde-hyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Nabarra B, Descamps B (1976) Ultrastructure of cells infiltrating human kidney allografts. Clin Exp Immunol 24:300–309
Nagura H, Koshikawa T, Fukuda Y, Asai J (1986) Hepatic vascular endothelial cells heterogenously express surface antigens associated with monocytes, macrophages and T lymphocytes. Virchows Arch A 409:407–416
Nagura H, Sumi Y (1987) Immunocytochemical studies of the vascular endothelial cells in human Peyer's patches and solitary lymphoid follicles. In: Mestecky J, McGhee JR (eds) Recent Development in Mucosal Immunology. Plenum Press, New York, pp 279–286
Paul LC, van Es LA, van Rood JJ, van Leeuwen A, de la Riviere GB, de Graeff J (1979) Antibodies directed against antigens on the endothelium of peritubular capillaries in patients with rejecting renal allografts. Transplantation 27:175–179
Rubinstein D, Roska AK, Lipsky PE (1986) Liver sinusoidal lining cells express class II major histocompatibility antigens but are poor stimulators of fresh allogeneic T lymphocytes. J Immunol 137:1803–1810
Rubinstein D, Roska AK, Lipsy PE (1987) Antigen presentation by liver sinusoidal lining cells after antigen exposure in vivo. J Immunol 138:1377–1382
Schreiner GF, Unanue ER (1984) Origin of the rat mesangial phagocyte and its expression of the leukocyte common antigen. Lab Invest 51:515–523
Schreiner GF, Cotran RS, Unanue ER (1984) Modulation of Ia and leukocyte common antigen expression in rat glomeruli during the course of glomerulonephritis and aminonucleoside nephritis. Lab Invest 51:524–533
Shen HH, Talle MA, Goldstein G, Chess L (1983) Functional subsets of human monocytes defined by monoclonal anti-bodies: A distinct subset of monocytes contains the cells capable of inducing the autologous mixed lymphocyte culture. J Immunol 130:698–705
Streefkerk JG (1972) Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol. J Histochem Cytochem 20:829–831
Striker GE, Mannik M, Tung MY (1979) Role of marrow-derived monocytes and mesangial cells in removal of immune complexes from renal glomeruli. J Exp Med 149:127–136
Takeuchi M, Nagura H, Kaneda T (1988) DDAVP and epinephrine induced changes in the localization of von Willebrand factor antigen in endothelial cells of human oral mucosa. Blood 72:850–854
Tilney NL, Garovoy MR, Busch GJ, Strom TB, Graves MJ, Charpenter CB (1979) Rejected human renal allografts: recovery and characteristics of infiltrating cells and antibody. Transplantation 28:421–426
Tipping PG, Holdsworth SR (1986) The participation of macrophages, glomerular procoagulant activity, and factor VIII in glomerular fibrin deposition. Am J Pathol 124:10–17
Yamamoto M, Shimokata K, Nagura H (1988) An immunohisto-chemical study on phenotypic heterogeneity of human pulmonary vascular endothelial cells. Virchows Arch A 412:479–486
Werber HI, Emancipater SN, Tykocinski ML, Sedor JR (1987) The interleukin 1 gene is expressed by rat glomerular mesangial cells and is augmented in immune complex glomerulonephritis. J Immunol 138:3207–3212
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kinjo, T., Takashi, M., Miyake, K. et al. Phenotypic heterogeneity of vascular endothelial cells in the human kidney. Cell Tissue Res. 256, 27–34 (1989). https://doi.org/10.1007/BF00224715
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00224715