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Bone marrow examination in the koala (Phascolarctos cinereus)

Abstract

A technique for bone marrow aspiration from the iliac crest in the koala (Phascolarctos cinereus) is described. Bone marrow was obtained from ten healthy koalas under general anaesthesia using the combination of tiletamine HCL and zolazepam HCL. Reference ranges were wide. The mean value for M:E was 1.7 (range 0.8–2.7), the mean percentage proliferating erythroid was 11% (range 5%–16%), and the mean percentage proliferating myeloid was 25% (range 17%–35%). In addition, bone marrow aspiration was performed on 14 koalas with various haematological diseases. Results showed aspiration to be useful in the diagnosis of leukaemia, and the investigation of regenerative anaemia and dysplastic anaemia. It was of varying use in the investigation of non-regenerative anaemia.

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References

  1. Backhouse TC, Bollinger A (1961) Morbidity and mortality in the koala (Phascolarctos cinereus). Aust J Zool 9:24–36

    Google Scholar 

  2. Batjer JD (1979) Preparation of optimal bone marrow samples. Lab Med 10:101–106

    Google Scholar 

  3. Bolliger A, Backhouse TC (1960) The blood of the koala (Phascolarctos cinereus). Aust J Zool 8:363–370

    Google Scholar 

  4. Bush M, Graves JAM, O'Brien SJ et al. (1990) Dissociative anaesthesia in free-ranging male koalas and selected marsupials in captivity. Aust Vet J 67:449–451

    Google Scholar 

  5. Canfield PJ, Spencer AJ (1993) Secondary degenerative arthropathy (osteoarthritis) of the hip joints in aging free-living koalas. Aust Vet J 70:394–395

    Google Scholar 

  6. Canfield PJ, Brown AS, Kelly WR et al. (1987) Spontaneous lymphoid neoplasia in the koala (Phascolarctos cinereus). J Comp Pathol 97:171–178

    Google Scholar 

  7. Canfield PJ, O'Neill ME, Smith EF (1989a) Haematological and biochemical investigations of diseased koalas (Phascolarctos cinereus). Aust Vet J 66:269–272

    Google Scholar 

  8. Canfield PJ, O'Neill ME, Smith EF (1989b) Haematological and biochemical reference values for the koala (Phascolarctos cinereus). Aust Vet J 66:324–326

    Google Scholar 

  9. Canfield PJ, Spencer AJ, Hartley W et al. (1992) Disorders of keratinisation in a group of related, captive koalas (Phascolarctos cinereus), with a review of other skin conditions in the koala. J Zoo Wild Med 23:414–412

    Google Scholar 

  10. Cartright GE (1966) The anemia of chronic disorders. Semin Hematol 3:351–375

    Google Scholar 

  11. Feldman BF, Kanecko JJ, Farver TB (1981) Anemia of inflammatory disease in the dog: clinical characterisation. Am J Vet Res 42:1109–1115

    Google Scholar 

  12. Gilmore CE, Gilmore VH, Jones TC (1964) Bone marrow and peripheral blood of cats: technique and normal values. Pathol Vet 1:18–40

    Google Scholar 

  13. Harvey JW (1984) Canine bone marrow: normal hematopoesis, biopsy technique, and cell identification and evaluation. Compendium on Continuing Education 10:909–925

    Google Scholar 

  14. Heuschele WP, Hayes JR (1961) Acute leukemia in a New South Wales koala. Cancer Res 21:1394–1395

    Google Scholar 

  15. Hoff B, Lumsden JH, Valli VEO (1985) An appraisal of bone marrow biopsy in assessment of sick dogs. Can J Comp Med 49:34–42

    Google Scholar 

  16. Jain NC (1986) Schalm's veterinary haematology. Lea and Febiger, Philadelphia, pp 12, 168

    Google Scholar 

  17. Latimer KS, Andreasen CB (1992) Bone marrow. In: Cowell RL, Tyler RD (eds) Cytology and hematology of the horse. American Veterinary Publications, California, p 209

    Google Scholar 

  18. Lee GR (1983) The anemia of chronic disease. Semin Hematol 20:61–76

    Google Scholar 

  19. Madewell BR (1986) Hematological and bone marrow cytological abnormalities in 75 dogs with malignant lymphoma. J Am Anim Hosp Assoc 22:235–240

    Google Scholar 

  20. McKenzie RA (1981) Observations on diseases of free-living and captive koalas (Phascolarctos cinereus). Aust Vet J 57:243–246

    Google Scholar 

  21. Melveger BE, Earl FL, Van Loon EJ (1969) Sternal bone marrow biopsy in the dog. Lab Anim Care 19:866–868

    Google Scholar 

  22. Penny RHC, Carlisle CH, Davidson HA (1970) The blood and bone marrow picture of the cat. Br Vet J 126:459–463

    Google Scholar 

  23. Spencer AJ, Canfield PJ (1993) Haematological characterisation of heavy tick infestations in the koala. (Phascolarctos Cinereus). Comp Haematol Int 3:225–229

    Google Scholar 

  24. Tyler RD, Cowell RL (1989). Bone marrow. In: Cowell RL, Tyler RD (eds) Diagnostic cytology of the dog and cat. American Veterinary Publications, California, pp 99–118

    Google Scholar 

  25. Watson ADJ (1979) Bone marrow failure in a dog. J Small Anim Pract 20:681–690

    Google Scholar 

  26. Wildt DE, O'Brien SJ, Graves JAM et al (1988) Anesthesia and reproductive characteristics of free-ranging male koalas (Phascolarctos cinereus). Proceedings Joint Conference, American Association of Zoo Veterinarians, Toronto, Ontario, pp. 109–111

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Correspondence to P. J. Canfield.

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Spencer, A.J., Canfield, P.J. Bone marrow examination in the koala (Phascolarctos cinereus). Comparative Haematology International 5, 31–37 (1995). https://doi.org/10.1007/BF00214488

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Keywords

  • Bone marrow aspiration
  • Koala
  • Phascolarctos cinereus
  • Anaemia
  • Leukaemia