Summary
The specific binding of 3H-quipazine to putative 5-HT3 receptors was analyzed in multiple species. Specific and saturable binding of the radioligand could be detected in both rat (K D = 1.2 nM; B max = 3.0 pmol/g) and pig (K D = 1.3 ± 0.2 nM; B maX = 1.5 ± 0.2 p/mol/g) cortical membranes. By contrast, no significant specific binding of 3H-quipazine could be detected in human, cow, dog, turtle, mouse, guinea pig, chicken or rabbit brain membranes. These data indicate that marked species variations exist in the presence and/or density of 5-HT3 membrane recognition sites in the central nervous system.
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Peroutka, S.J. Species variations in 5-HT3 recognition sites labeled by 3H-quipazine in the central nervous system. Naunyn-Schmiedeberg's Arch Pharmacol 338, 472–475 (1988). https://doi.org/10.1007/BF00179316
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DOI: https://doi.org/10.1007/BF00179316