Abstract
A Namalwa cell line, KJM-1, which was adapted to serum-free medium is thought to be a good host cell line for recombinant DNA technology. We previously reported the expression of human β-interferon (β-IFN) in Namalwa KJM-1 (Miyaji, 1989a). The utility of Namalwa KJM-1 for expression of foreign genes was further examined. As a target gene to be expressed, human lymphotoxin (hLT) cDNA was used. It was engineered for expression in Namalwa KJM-1 using a simian virus 40 (SV40)-based expression vector pAGE107 (Miyaji, 1989a). It contains all components necessary for the expression of cDNA in mammalian cells. The expression vector was introduced into Namalwa KJM-1 by electroporation. Among the transformants, clone 7 was further examined for the expression of hLT in serum-free medium. The production level of hLT was augmented with the increase of the cell density. Thus it was further indicated that Namalwa KJM-1 is useful for production of foreign gene products.
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Abbreviations
- HEPES:
-
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
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Miyaji, H., Harada, N., Mizukami, T. et al. Expression of human lymphotoxin in Namalwa KJM-1 cells adapted to serum-free medium. Cytotechnology 4, 39–43 (1990). https://doi.org/10.1007/BF00148809
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DOI: https://doi.org/10.1007/BF00148809