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Cytotoxicity of D-penicillamine in association with several heavy metals against cultured rabbit articular chondrocytes

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Abstract

The potentiation or antagonistic effects of Cu, Hg, Pb and Cd salts in the presence of a long-acting anti-rheumatic drug, D-penicillamine (D.P.) were studied on cultured chondrocytes. CuSO4 (10−4M), HgCl2 (10−5M), Pb(CH3COO)2 (10−3M) and D.P. (10−3M) when used alone caused a small decrease in cell proliferation. The addition of D.P. with Cu, Hg or Pb salts resulted in a marked increase in the extent of growth inhibition. In contrast, CdCl2 (10−5M) produced an important growth inhibitory effect, and D.P. antagonized CdCl2 action. The CuSO4 D.P. toxicity was probably due to production of H2O2 in situ. To verify this hypothesis, catalase, responsible for H2O2 metabolism was used, and was found to partially reverse the inhibitory effect of CuSO4-D.P.

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Authors and Affiliations

  1. Laboratoire de Pharmacologie Cellulaire de l'Ecole Pratique des Hautes Etudes, 15 Rue de l'Ecole de Médecine, 75006, Paris, France

    Elisabeth Clain, Nicole Farjaudon, Hervé Brun & Monique Adolphe

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  1. Elisabeth Clain
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  2. Nicole Farjaudon
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  3. Hervé Brun
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  4. Monique Adolphe
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Clain, E., Farjaudon, N., Brun, H. et al. Cytotoxicity of D-penicillamine in association with several heavy metals against cultured rabbit articular chondrocytes. Cytotechnology 1, 139–144 (1988). https://doi.org/10.1007/BF00146814

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