Skip to main content

Nitrogen fixation throughout growth, and varietal differences in nitrogen fixation by the rhizosphere of rice planted in pots


The nitrogen-fixing activity in the rhizospheres of various rices was measured by the acetylene-reduction method throughout plant growth in green-house pots. The activity began to increase 4 weeks after transplanting, increased until heading stage, then decreased. The concentrations of exchangeable ammonium and sugars in the soil were not related to the variation of nitrogen-fixing activity during rice growth.

The nitrogen-fixing activities in the rhizospheres of 41 rice varieties in pots were measured to discover varietal differences. Levels of nitrogen fixation were highly correlated with the rices' dry root weight at heading stage.

This is a preview of subscription content, access via your institution.


  1. 1

    Dommergues, Y., Balandreau, J., Rinaudo, G. and Weinhard, P., Non-symbiotic nitrogen fixation in the rhizosphere of rice, maize and different tropical grasses. Soil Biol. Biochem. 5, 83–89 (1973).

    Google Scholar 

  2. 2

    Lee, K. K. and Yoshida, T., An assay technique of measurement of nitrogenase activity in root zone of rice for varietal screening by the acetylene reduction method. Plant and Soil 46, 127–134 (1977).

    Google Scholar 

  3. 3

    Rinaudo, G., Balandreau, J. and Dommergues, Y., Algal and bacterial nonsymbiotic nitrogen fixation in paddy soils. Plant and Soil (Spec. Vol.), 471–479 (1971).

  4. 4

    Yoshida, S., Forno, D. A., Cock, J. H. and Gomez, K. A., Laboratory Manual for Physiological Studies of Rice. 2nd ed. The International Rice Research Institute, Los Baños, Philippines, 70 pp. (1972).

    Google Scholar 

  5. 5

    Yoshida, T. and Ancajas, R. R., Nitrogen fixation by bacteria in the root zone of rice. Soil Sci. Soc. Am. Proc. 35, 156–157 (1971).

    Google Scholar 

  6. 6

    Yoshida, T. and Ancajas, R. R., Nitrogen-fixing activity in upland and flooded rice fields. Soil Sci. Soc. Am. Proc. 37, 42–46 (1973).

    Google Scholar 

  7. 7

    Yoshida, T. and Broadbent, F. E., Movement of atmospheric nitrogen in rice plants. Soil Sci. 120, 288–291 (1975).

    Google Scholar 

  8. 8

    Yoshida, T., Takai, Y. and Del, Rosario, D. C., Molecular nitrogen content in a submerged rice field. Plant and Soil 42, 653–660 (1975).

    Google Scholar 

Download references

Author information



Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lee, KK., Castro, T. & Yoshida, T. Nitrogen fixation throughout growth, and varietal differences in nitrogen fixation by the rhizosphere of rice planted in pots. Plant Soil 48, 613–619 (1977).

Download citation


  • Nitrogen
  • Ammonium
  • Sugar
  • Plant Growth
  • Plant Physiology