Abstract
The culture kinetics of human tumor kidney cells (TCL 598) grown on microcarriers are compared with media initially supplemented with either glucose alone or a mixture of galactose and glucose. Growth rates and maximal cell densities are similar, but cellular death is much slower in galactose than in glucose. Galactose is metabolized at a much slower specific rate than glucose. Cells grown in the galactose medium show a different pattern of lactate and pyruvate metabolism compared to cells grown in the glucose medium. Growth with galactose also favours oxidation of glutamine to alanine.
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Wagner, A., Marc, A., Engasser, J.M. et al. Growth and metabolism of human tumor kidney cells on galactose and glucose. Cytotechnology 7, 7–13 (1991). https://doi.org/10.1007/BF00135633
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DOI: https://doi.org/10.1007/BF00135633