Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 31, Issue 1, pp 81–86 | Cite as

Effects of autoclave-induced carbohydrate hydrolysis on the growth ofBeta vulgaris cells in suspension

  • Heidi Sawyer
  • Ke-Cheng Hsiao
Article
  • 151 Downloads

Abstract

Media of de Greef & Jacobs (1979) were autoclaved either with all the nutrient components in a single vessel (medium 1) or with the following components in separate vessels: FeNa−EDTA+CaCl2 (medium 2), FeNa−EDTA+NaH2PO4 (medium 3) or sucrose (medium 4). Medium 5 was prepared by autoclaving FeNa−EDTA+NaH2PO4 and sucrose in two separate vessels. It was found that the dry mass yield of cell suspensions ofBeta vulgaris was lowest in medium 1, followed by media 2 and 3. There was no significant difference among media 3, 4, and 5.

The plot of dry mass yield of the cell suspensions against the rates of cyanide-initiated oxygen consumption which indicate the extent of carbohydrate hydrolysis of the media during autoclaving, indicated the presence of a threshold rate of about 17–20 nmol ml−1 min−1. Dry mass yield of the suspensions decreased rapidly when the rate exceeded this value.

For media with glucose as the source of carbohydrate, the rate of cyanide-initiated oxygen consumption exceeded the threshold value by a factor of 1.5 to 2, depending on the volume of the media autoclaved.

Key words

autoclaving Beta vulgaris carbohydrate hydrolysis cell suspension cyanide FeNa-EDTA medium toxicity oxygen consumption 

Abbreviations

FeNa-EDTA

ferric monosodium ethylenediamine-tetraacetic acid

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Heidi Sawyer
    • 1
  • Ke-Cheng Hsiao
    • 1
  1. 1.Research and DevelopmentHilleshög ABLandskronaSweden

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