Abstract
Embryos extracted from dried seeds of common ash (Fraxinus excelsior), were germinated on growth regulator-free culture medium. Cotyledonary nodes from these seedlings were placed onto Murashige and Skoog, Woody Plant or Driver and Kuniyuki culture media with 22.2 or 44.4 μM benzyladenine, on which they developed into shoot cultures following the outgrowth of axillary buds. With Murashige and Skoog medium, cultures often died. With Woody Plant Medium, survival of the cultures was considerably improved, but large amounts of callus were produced at the cut ends of the explants, and new axillary shoots had long internodes and small leaves. With Driver and Kuniyuki medium, both survival and callus formation were much improved, and the shoots produced were of high quality. Proliferation of axillary shoots was obtained from both shoot tip and nodal explants placed onto Driver and Kuniyuki medium with 22.2 μM benzyladenine. Adventitious root formation was best with shoots inserted into half-strength Woody Plant Medium containing 2.45, 4.9 or 9.8 μM indolebutyric acid. All of the rooted plantlets tested have successfully established in soil.
Similar content being viewed by others
References
Barghchi M & Alderson PG (1985)In vitro Propagation ofPistacia vera L. and the commercial cultivars Ohadi and Kalleghochi. J. Hort. Sci. 60: 423–430
Brazier JD (1990) The Timbers of Farm Woodland Trees. Forestry Commission Bulletin 91. Her Majesty's Stationery Office, London
Chalupa V (1990) Micropropagation of hornbeam (Carpinus betulus L.) and ash (Fraxinus excelsior L.). Biol. Plant. 32: 332–338
Cox DR & Snell EJ (1989) Analysis of Binary Data, 2nd Edition. Chapman and Hall, London
Driver JA & Kuniyuki AH (1984)In vitro propagation of Paradox walnut rootstock. HortScience 19: 507–509
Linington IM (1991)In vitro propagation ofDipterocarpus alatus andDipterocarpus intricatus. Plant Cell Tiss. Org. Cult. 27: 81–88
Lloyd G & McCown B (1980) Commercially-feasible micropropagation of mountain laurel,Kalmia latifolia, by use of shoot tip culture. Comb. Proc. Intl. Plant Prop. Soc. 30: 421–427
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Preece JE, Christ PH, Ensenberger L & Zhao J-L (1987) Micropropagation of ash (Fraxinus). Comb. Proc. Intl. Plant Prop. Soc. 37: 366–372
Preece JE, Zhao J-L & Kung FH (1989) Callus production and somatic embryogenesis from white ash. HortScience 24: 377–380
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hammatt, N., Ridout, M.S. Micropropagation of common ash (Fraxinus excelsior). Plant Cell Tiss Organ Cult 31, 67–74 (1992). https://doi.org/10.1007/BF00043477
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00043477