Cell-suspension cultures ofSpinacia andRosa accumulated a cell wall protein, extensin, in a form that was amenable to leaching from the surface of the living cells by a brief treatment with non-toxic salts. Cultures ofLycopersicon, Capsicum, Acer andFestuca did not accumulate this class of extensin. InSpinacia andRosa, optimum yields of leachable extensin were achieved from young cultures, in media at relatively low pH, by leaching with 0.1 M CaCl2. Older cultures, pH values >6.5, and LaCl3 or higher concentrations of CaCl2 were less effective.
cell wall-bound protein extensin salt-leaching Spinacia oleraceaRosa sp. yield
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