Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 31, Issue 1, pp 61–66 | Cite as

Production and harvesting of ionically wall-bound extensin from living cell suspension cultures

  • Janice G. Miller
  • Stephen C. Fry


Cell-suspension cultures ofSpinacia andRosa accumulated a cell wall protein, extensin, in a form that was amenable to leaching from the surface of the living cells by a brief treatment with non-toxic salts. Cultures ofLycopersicon, Capsicum, Acer andFestuca did not accumulate this class of extensin. InSpinacia andRosa, optimum yields of leachable extensin were achieved from young cultures, in media at relatively low pH, by leaching with 0.1 M CaCl2. Older cultures, pH values >6.5, and LaCl3 or higher concentrations of CaCl2 were less effective.

Key words

cell wall-bound protein extensin salt-leaching Spinacia oleracea Rosa sp. yield 



trichloroacetic acid


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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Janice G. Miller
    • 1
  • Stephen C. Fry
    • 1
  1. 1.Centre for Plant Science, Division of Biological SciencesUniversity of EdinburghEdinburghUK

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