Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 31, Issue 1, pp 51–59 | Cite as

The influence of bud length, age of the tree and culture media on androgenesis induction inAesculus carnea Hayne anther culture

  • Nevena Marinkovié
  • Ljiljana Radojevié
Article
  • 31 Downloads

Abstract

Statistical analyses of the data revealed very significant differences in androgenesis induction ofA. carnea Hayne anther culture depending on the bud length, nutrient medium composition and age of the parental tree. Significant mutual influence of all these factors was also observed. The highest number of androgenic anthers was obtained when 4 mm long buds were used. Older trees (60 and 100 yrs) gave a higher number of androgenic anthers than the younger ones (20 and 40 yrs). MS medium supplemented with 2,4-d and Kin (1 mg l−1, each) was the most favourable for androgenesis induction. Pollen embryos (haploids and aneuploids) were formed by the division of uninuclear microspores.

The highest percentage of germinated embryos and further synchronous development of the shoot and root was achieved in MS medium supplemented with IAA, GA3 (1 mg l−1) and activated charcoal (1%). When other germination media were used, malformations of androgenic embryos were observed.

Key words

Aesculus carnea androgenesis androgenic embryos anther culture bud length culture media 

Abbreviations

AC

activated charcoal

H

casein hydrolysate

2,4-d

2,4-dichlorophenoxyacetic acid

IAA

indole-3-acetic acid

IBA

indole-3-butyric acid

BAP

6-benzylaminopurine

GA3

gibberelic acid

Kin

6-furfurylaminopurine

MS

Murashige and Skoog

T

thidiazurone

N

phenyl-N'-1,2,3-thiadiazol-5-ylurea

Z

zeatin-6-(4-hydroxy-3-methyl-trans-2-butenylamino)purine

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Nevena Marinkovié
    • 1
  • Ljiljana Radojevié
    • 1
  1. 1.Department of Plant PhysiologyThe Institute for Biological Research ‘Siniša Stanković’BelgradeMacedonia

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