Somatic embryogenesis and high frequency plantlet regeneration in callus cultures ofThevetia peruviana
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Plantlet regeneration through somatic embryogenesis has been achieved in the apocynaceous medicinal treeThevetia peruviana L. Calluses obtained by culturing young leaf discs on MS medium containing 9 μM 2,4-dichlorophenoxyacetic acid and 4.6 μM kinetin, when subjected to reduced levels of the growth regulators followed by higher cytokinin treatment, produced numerous somatic embryos. Somatic embryos developed into complete plantlets on a medium devoid of growth regulators. An average of 40–50 plantlets were obtained from 50 mg of embryogenic callus. Survival of transplants was 60% under glasshouse conditions.
Key wordsembryoids leaf callus medicinal tree
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