Abstract
Stereological techniques were used to quantify ultrastructural changes which occurred during maturation of cultured Paul's Scarlet rose cells. The volume and ultrastructural composition of young, dividing, unsynchronized 5-day-old cells were compared to that of mature, nondividing 14-day-old cells. The volume of the 14-day-old cells was 4-fold greater than that of the 5-day-old cells, primarily due to vacuole expansion. Numerous quantitative changes occurred in the organelle composition during cell maturation, but distinctive differences were observed in the magnitude and direction of change among the different types of organelles. There was an overall decline in the plastid population as measured by both percent of cell volume and numbers of plastids per cell. The percent of cell volume and numbers of lipid bodies increased, whereas the percent volume of the mitochondria remained relatively constant while the number per cell declined.
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References
Berlin J, Quisenberry JE, Bailey F, Woodworth M & McMichael BL (1982) Effect of water stress on cotton leaves. Plant Physiol. 70: 238–243
Briarty LG (1980) Stereological analysis of cotyledon cell development inPhaseolus II. The developing cotyledon. J. Exp. Bot. 31: 1387–1398
Davey MR & Street HE (1971) Studies on the growth in culture of plant cells. J. Exp. Bot. 22: 90–95
Fletcher JS & Beevers H (1970) Acetate metabolism in cell suspension cultures. Plant Physiol. 45: 765–772
Forde J & Steer MW (1976) The use of quantitative electron microscopy in the study of lipid composition of membranes. J. Exp. Bot. 27: 1137–1141
Fowke LC (1986) Ultrastructural cytology of cultured plant tissues, cells and protoplasts. In: Vasil IK (Ed) Cell Culture and Somatic Cell Genetics of Plants, Vol 3 (pp 323–342). Academic Press, Orlando
Galey FR & Nilsson SEG (1966) A new method for transferring sections from the liquid surface of the trough through staining solutions to the supporting film of a grid. J. Ultrastruct. Res. 14: 405–410
Hadley NF (1985) The Adaptive Role of Lipids in Biological Systems (pp 42–43). John Wiley and Sons Inc., New York
Hajibagheri MS, Hall JL & Flowers TJ (1984) Stereological analysis of leaf cells of the halophyteSuaeda maritima (L.) Dum. J. Exp. Bot. 35: 1547–1557
Hunt L & Fletcher JS (1976) Estimated drainage of carbon from the tricarboxylic acid cycle for protein synthesis in suspension cultures of Paul's Scarlet rose cells. Plant Physiol. 57: 304–307
Jones IH, Barrett JN & Gopal PPS (1973) Growth and nutrition of a suspension culture ofPogostemon cablin Benth. (Patchouli). J. Exp. Bot. 24: 145–158
King PJ & Street HE (1973) Growth patterns in cell cultures. In: Street HE (Ed) Plant Tissue and Cell Culture (pp 269–337). Univ. California, Press, Berkeley
Kirk JD (1967) The Plastid (p 63). W.H. Freeman and Co., London
Kunce CM, Trelease RN & Doman DC (1984) Ontogeny of glyoxysomes in cotton seeds-a morphometric analysis. Planta 161: 156–164
Ledbetter MC & Porter KR (1970) Introduction to the Fine Structure of Plant Cells (p 9). Springer-Verlag, Berlin
Mauseth JD (1980) A morphometric study of the ultrastructure ofEchinocereus engelmannii (Cactaceae) I. Shoot apical meristems at germination. Amer. J. Bot. 67: 173–181
Mohanty B & Fletcher JS (1980) Ammonium influence on nitrogen assimilating enzymes and protein accumulation in suspension cultures of Paul's Scarlet rose. Physiol. Plant. 48: 453–459
Nesius KK, Uchytil LE & Fletcher JS (1972) Minimal organic medium for suspension cultures of Paul's Scarlet rose. Planta 106: 173–176
Nesius KK & Fletcher JS (1975) Contribution of nonautotrophic carbon dioxide fixation to protein synthesis in suspension culture of Paul's Scarlet Rose. Plant Physiol. 55: 643–645
Reider ML, Smith BA & Fletcher JS (1982) Protein content and subculturing properties of senescing plant suspension cultures. In Vitro 82: 1004–1008
Sutton-Jones B & Street HE (1968) Studies on the growth in culture of plant cells. J. Exp. Bot. 19: 114–118
Thimann KV (1980) The senescence of leaves. In: Thimann KV (Ed) Senescence in Plants (pp 85–115). CRC Press, Boca Raton
Torrey JG (1967) Morphogenesis in relation to chromosomal constitution in long-term plant tissue culture. Physiol. Plant 20: 265–275
Toth R (1982) An introduction to morphometric cytology and its application to botanical research. Amer. J. Bot. 69: 1694–1706
Tulecke W (1963) Research on tissue cultures of higher plants. Technical report AD-431–213 (pp 63–124). Aerospace medical research laboratory, Wright-Patterson AFB, Ohio
Wallsgrove RM, Lea PJ & Miflin BJ (1983) Intracellular localization of aspartate kinase and the enzymes of threonine and methionine biosynthesis in green leaves. Plant Physiol. 71: 780–784
Weibel ER & Bolender RP (1973) Stereological techniques for electron microscopic morphometry. In: Hayat MA (Ed) Principles and Techniques of Electron Microscopy, Biological Applications, Vol 3 (pp 237–296). Van Nostrand Reinhold Co., New York
Weibel ER (1979) Stereological Methods (pp 91–100). Academic Press, London
Young SJ, Royer SM, Groves PM & Kinnamon JC (1987) Three-dimensional reconstruction from serial micrographs using the IBM PC. J. Electron Microsc. Tech. 6: 207–217
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Watson, B.S., Fletcher, J.S. & Russell, S.D. Quantification of organelle changes in plant suspension cultures during growth. Plant Cell Tiss Organ Cult 31, 37–46 (1992). https://doi.org/10.1007/BF00043473
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DOI: https://doi.org/10.1007/BF00043473