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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 31, Issue 1, pp 31–35 | Cite as

The effect of asparagus virus infection on asparagus tissue culture

  • R. M. De Vries-Paterson
  • T. A. Evans
  • C. T. Stephens
Article

Abstract

The impact of asparagus virus I (AV-I), a potyvirus, and asparagus virus II (AV-II), an ilarvirus, on micropropagation of field-grown asparagus was studied. Apical shoot tips excised from singly or doubly-infected plants were slow to develop roots and had a 15 to 75% reduction in survival in culture, respectively, compared to those excised from virus-free plants. The four virus infection groups were ranked: virus-free >AV-II>AV-I>AV-I & II for capacity of explants to both root and survivein vitro. Micropropagated plants infected with AV-II exhibited slight reductions in fresh and dry weights, with greater reductions associated with infection with AV-I and double infection, compared to the virus-free controls. Eighty-one virus-infected apical shoot tips yielded 7 (8.6%) virus-free clones, as determined by rub inoculation on indicator plants.

Key words

Asparagus officinalis asparagus virus I asparagus virus II micropropagation 

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References

  1. Bertaccini A, Marani F & Passarelli V (1984) Risanamento dell'asparago dall'infezione da virus 2. Atti Giorn. Fitopatol 3: 437–447Google Scholar
  2. Chin C (1982) Promotion of shoot and root formation in asparagusin vitro by ancymidol. HortScience 17: 590–591Google Scholar
  3. Davis RF & Garrison S (1984) First report of a virus present in asparagus in New Jersey. Plant Dis. 68: 628CrossRefGoogle Scholar
  4. Derrick KS (1973) Quantitative assay for plant viruses using serologically specific electron microscopy. Virology 56: 652–653CrossRefPubMedGoogle Scholar
  5. De Vries-Paterson RM, Stephens CT & Evans TA (1990) The effect of asparagus virus infection on the rooting and survival of asparagus tissue culture clones. HortScience 25: 625Google Scholar
  6. Evans TA & Stephens CT (1986) Effect of virus infection on root initiation and survival of asparagus tissue culture clones. Phytopathology 72: 1134Google Scholar
  7. Evans TA & Stephens CT (1989) Increased susceptibility toFusarium crown and root rot in virus-infected asparagus. Phytopathology 79: 253–258CrossRefGoogle Scholar
  8. Evans TA & Stephens CT (1989) First report of asparagus virus I in asparagus in Michigan. Phytopathology 73: 273Google Scholar
  9. Evans TA, De Vries RM, Wacker TL & Stephens CT (1990) Epidemiology of asparagus viruses in Michigan asparagus. Acta Hort. 271: 285–290CrossRefGoogle Scholar
  10. Faccioli G (1965) Ulteriori studi su di un virus isolato daAsparagus officinalis L. Phytopathol Mediterr 4: 163–167 (Rev. Appl. Mycol. 46: 158. 1967)Google Scholar
  11. Falloon PG, Falloon LM & Grogan RG (1986) Survey of California asparagus for asparagus virus I, asparagus virus II and tobacco streak virus. Plant Dis. 70: 103–105CrossRefGoogle Scholar
  12. Fujisawa I, Goto T, Tsuchizaki T & Iizuka N (1983) Some properties of asparagus virus II isolated fromAsparagus officinalis. Jpn Ann. Phytopathol. Soc. Jpn 49: 683–688CrossRefGoogle Scholar
  13. Greno V, Navarro L & Duran-Vila N (1988) Influence of virus and virus-like agents on the development of citrus buds culturedin vitro. Plant Cell Tiss. Org. Cult. 15: 113–124CrossRefGoogle Scholar
  14. Hartung AC, Evans TA & Stephens CT (1985) Occurrence of asparagus virus II in commercial asparagus fields in Michigan. Plant Dis. 69: 501–504CrossRefGoogle Scholar
  15. Hein A (1963) Viroses am spargel. Mitt. Biol. Bundesanst. Land-Forstwirtsch, Berlin-Dahlem 108: 70–74 (Rev. Appl. Mycol. 1964 43: 455)Google Scholar
  16. Hein A (1969) Über viruskrankheiten das spargels (Asparagus officinalis L.). Sparge virus I Z Pflanzenkr Pflanzenpathol Pflanzenschutz 76: 395–406 (Rev. Plant Pathol. 1970 49: 219)Google Scholar
  17. Mellor FC & Stace-Smith R (1977) Virus-free potatoes by tissue culture. In: Reinert J & Bajaj YPS (Eds) Applied and Fundamental Aspects of Plant Cell Tissue and Organ Culture (pp 616–635). Springer-Verlag, BerlinGoogle Scholar
  18. Mink GI & Uyeda I (1977) Three mechanically transmissible viruses isolated from asparagus in Washington. Plant Dis. Rept. 61: 398–401Google Scholar
  19. Montasser MS & Davis RF (1987) Survey for asparagus viruses I and II in New Jersey. Plant Dis. 71: 497–499CrossRefGoogle Scholar
  20. Montasser MS, Davis RF & Chin CK (1987) Effect of antiviral chemicals and heat treatment on production of virus-free asparagus clones. Phytopathology 77: 120–121Google Scholar
  21. Murakishi HM (1962) Inhibition of callus and root growth in tomato stems excised form plants inoculated with a combination of tobacco mosaic virus and potato virus X. Phytopathology 52: 745Google Scholar
  22. Murashige T & Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497CrossRefGoogle Scholar
  23. Paludan N (1964) Virussygdomme hosAsparagus officinalis. Maanedsovers Plantesygd. 407: 11–16Google Scholar
  24. Posnette AF (1969) Nematode-transmitted viruses in asparagus. J. Hort. Sci. 44: 403–406CrossRefGoogle Scholar
  25. Schade C (1969) Viruskrankheiten des spargels Nachrichtenbl Dtsch Pflanzenschutzdienst (Berl) 23: 38–40Google Scholar
  26. Steel RGD & Torrie JH (1980) Principles and Procedures of Statistics. McGraw-Hill Book Co Inc, New YorkGoogle Scholar
  27. Uyeda I & Mink GI (1981) Properties of asparagus virus II, a new member of the ilarvirus group. Phytopathology 71: 1264–1269Google Scholar
  28. Weissenfels M & Schmelzer K (1976) Untersuchungen über das Schadansmass durch Viren am Spargel (Asparagus officinalis L.). Arch. Phytopath. U. PflSchutz. Berlin 12: 67–73CrossRefGoogle Scholar
  29. Wilson HM & Eisa MZ (1975) The effects of potato viruses X and Y on organogenesis in tobacco tissue cultures. Plant Sci. Lett. 5: 395–400CrossRefGoogle Scholar
  30. Yang HJ (1979) Early effects of viruses on the growth and productivity of asparagus plants. HortScience 14: 734–735Google Scholar
  31. Yang HJ & Clore WJ (1976) Obtaining virus-free plants ofAsparagus officinalis L. by culturing shoot tips and apical meristems. HortScience 11: 474–475Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • R. M. De Vries-Paterson
    • 1
  • T. A. Evans
    • 2
  • C. T. Stephens
    • 1
  1. 1.Department of Botany and Plant PathologyMichigan State UniversityEast LansingUSA
  2. 2.Department of Plant and Soil SciencesUniversity of Delaware, Agricultural Research and Education CenterGeorgetownUSA

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