Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 31, Issue 1, pp 31–35 | Cite as

The effect of asparagus virus infection on asparagus tissue culture

  • R. M. De Vries-Paterson
  • T. A. Evans
  • C. T. Stephens


The impact of asparagus virus I (AV-I), a potyvirus, and asparagus virus II (AV-II), an ilarvirus, on micropropagation of field-grown asparagus was studied. Apical shoot tips excised from singly or doubly-infected plants were slow to develop roots and had a 15 to 75% reduction in survival in culture, respectively, compared to those excised from virus-free plants. The four virus infection groups were ranked: virus-free >AV-II>AV-I>AV-I & II for capacity of explants to both root and survivein vitro. Micropropagated plants infected with AV-II exhibited slight reductions in fresh and dry weights, with greater reductions associated with infection with AV-I and double infection, compared to the virus-free controls. Eighty-one virus-infected apical shoot tips yielded 7 (8.6%) virus-free clones, as determined by rub inoculation on indicator plants.

Key words

Asparagus officinalis asparagus virus I asparagus virus II micropropagation 


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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • R. M. De Vries-Paterson
    • 1
  • T. A. Evans
    • 2
  • C. T. Stephens
    • 1
  1. 1.Department of Botany and Plant PathologyMichigan State UniversityEast LansingUSA
  2. 2.Department of Plant and Soil SciencesUniversity of Delaware, Agricultural Research and Education CenterGeorgetownUSA

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