Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 31, Issue 1, pp 21–29 | Cite as

Thidiazuron stimulates shoot organogenesis and somatic embryogenesis in white ash (Fraxinus americana L.)

  • Sharon Bates
  • John E. Preece
  • Nadia E. Navarrete
  • J. W. Van Sambeek
  • Gerald R. Gaffney
Article

Abstract

Immature and mature nonstratified seeds of white ash (Fraxinus americana L.) were dissected transversely and 2/3 of each seed was placed onto agar-solidified Murashige and Skoog medium. Adventitious buds, shoots, and somatic embryos formed on callus, cotyledons, and hypocotyls of the resulting seedlings. Shoot organogenesis was induced on explants cultured on medium with 10 μM thidiazuron but not on explants on media with benzyladenine (BA) or isopentenyladenine. Not all seed sources were equally capable of shoot organogenesis and embryogenesis. Atypical of adventitious regeneration of other woody plants, mature seed explants of white ash were more organogenic with shoots that elongated better than explants from immature seeds. Somatic embryogenesis was observed in cultures where mature seeds were first cultured for 4 weeks on a medium containing 10 μM adenine 2,4-dichlorophenoxyacetic acid in combination with 0.1 and 1.0 μM thidiazuron, followed by transfer to a medium containing 0.05 μM 6-benzyladenine and 0.5 μM naphthaleneacetic acid. Adventitious shoots and epicotyls from both seedlings and germinated somatic embryos were rooted under intermittent mist and acclimatized to the greenhouse.

Key words

glyphosate in vitro micropropagation regeneration tree woody plant tissue culture 

Abbreviations

BA

6-benzyladenine

2,4-d

2,4-dichlorophenoxyacetic acid

IBA

indolebutyric acid

2iP

isopentenyladenine

NAA

naphthaleneacetic acid

TDZ

thidiazuron-N-phenyl-N′-1,2,3-thiadiazol-5-ylurea

WPM

woody plant medium

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Sharon Bates
    • 1
  • John E. Preece
    • 1
  • Nadia E. Navarrete
    • 2
  • J. W. Van Sambeek
    • 3
  • Gerald R. Gaffney
    • 2
  1. 1.Department of Plant and Soil ScienceSouthern Illinois UniversityCarbondaleUSA
  2. 2.Department of ForestrySouthern Illinois UniversityCarbondaleUSA
  3. 3.North Central Forest Experiment StationUSDA Forest ServiceCarbondaleUSA

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