Effect ofin vitro storage at 4°C on survival and proliferation of two apple rootstocks

  • Teresa Orlikowska


One cm long shoot explants of dwarf apple rootstocks P 2 and M.9 taken from 2 year-old cultures were stored at 4°C in the dark in three media differing in concentration of growth regulators. Every 6 weeks, some explants were transferred into proliferation medium and multiplication rate was observed during three or four consecutive passages. In a second experiment, the influence of explant type (1 cm long shoot tips, 1 cm long middle part of shoots or three-shoot tufts smaller than 1 cm) and transfer time to the cold room (immediately, 10 days, or 20 days after subculture) on explant survival and proliferation were analysed.

Survival of explants was influenced by composition of the storage media. On medium without 6-benzylaminopurine, 70% of P 2 and 17% of M.9 explants became necrotic during 18 weeks of storage. P 2 rootstock proliferated better in three passages after storage than did unstored controls. Storage of M.9 rootstock reduced proliferation in the first and second passages if stored in media containing 6-benzylaminopurine in comparison with unstored controls. Explants stored as tufts and transferred to the cold room directly after subculture produced more shoots during two passages than cultures stored as single shoots.

Key words

apple rootstocks in vitro storage shoot proliferation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aitken-Christie J & Singh AP (1987) Cold storage of tissue culture. In: Bonga JM & Durzan DJ (Eds) Cell and Tissue Culture in Forestry, Vol 2 (pp 285–304). Martinus Nijhoff Publishers, DordrechtCrossRefGoogle Scholar
  2. Borkowdka B (1990) Influence of low temperature storage on regenerative capacity of sour cherry cultivars. Fruit Sci. Rep. 17: 1–7Google Scholar
  3. Eckhard A (1989) Untersuchungen zur Entwicklung einer rationellen Methode derIn-vitro-Depothaltung von Kernund Steinobst unter Kuhlbedingungen. Arch Gartenbau 2: 131–140Google Scholar
  4. Lundergan C & Janick J (1979) Low temperature storage ofin vitro apple shoots. HortScience 14: 514Google Scholar
  5. Marino G, Rosati P & Sagrati F (1985) Storage ofin vitro cultures ofPrunus rootstocks. Plant Cell Tiss. Org. Cult. 5: 73–78CrossRefGoogle Scholar
  6. Monette PL (1986) Cold storage of kiwifruit shoot tipsin vitro. HortScience 21: 1203–1205Google Scholar
  7. Monette PL (1988) Grapevine (Vitis vinifera L.) In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry, Vol 6 Crops II (pp 3–37). Springer VerlagCrossRefGoogle Scholar
  8. Mullin RH & Schlegel DE (1976) Cold storage maintenance of strawberry meristem plantlets. HortScience 11: 100–101Google Scholar
  9. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497CrossRefGoogle Scholar
  10. Orlikowska T (1991) Propagationin vitro of P 60-new Polish clonal apple rootstock. Fruit Sci. Rep. 18: 1–5Google Scholar
  11. Wanas WH, Callow JA & Withers LA (1986) Growth limitation for the conservation of pear genotypes. In: Withers LA & Alderson PG (Eds) Plant Tissue Culture and its Agricultural Applications (pp 285–290). ButterwothsCrossRefGoogle Scholar
  12. Welander M (1988) Gooseberry (Ribes uva-crispa (grossularia)). In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry, Vol 6 Crops II (pp 156–165). Springer VerlagCrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Teresa Orlikowska
    • 1
  1. 1.Research Institute of Pomology and FloricultureSkierniewicePoland

Personalised recommendations