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Powdery mildew resistance in Kentucky bluegrass genotypes regenerated from excised seed pieces

Summary

Severity of powdery mildew was assessed on seven cultivars and lines of Kentucky bluegrass propagated by seed and tissue culture. Tissue culture plants were started from embryo axes cultured on Murashige and Skoog medium containing different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP), and incubated (1 to 4 weeks) or not incubated in the dark prior to transfer to a lighted culture room. There were significant differences in disease severity (DS) among seed propagated and tissue culture regenerated plants. DS ranged from highly susceptible (100% of leaf covered by mildew) (DS=9) to resistant (DS=3.0). In some tissue culture regenerants the disease severity was significantly affected by the tissue culture process. Ten clones expressing resistance were selected, and plants propagated vegetatively. In six clones, disease resistance was sustainable in subsequent vegetatively propagated plants, while resistance was lost in four of the selected clones. Results are discussed with a view to using tissue culture to produce Kentucky bluegrass genotypes with resistance to powedery mildew.

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Abbreviations

BAP:

benylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

DS:

disease severity

MS:

Murashige & Skoog

SDW:

sterile distilled water

References

  1. Ayers, J.E. & B.K., Gilmore, 1991. Fungicide Benefits Assessment-Turf. USDA National Agricultural Pesticide Impact Assessment Program, Washington, D.C.

    Google Scholar 

  2. Bajaj, Y.P.S., 1990. Somaclonal variation-origin, induction, cryopreservation, and implications in plant breeding. In: Y.P.S., Bajaj (Ed.). Biotechnology in Agriculture and Forestry. Vol. 11. Somaclonal Variation in Crop Improvement, pp. 13–48. Springer-Verlag, New York.

    Google Scholar 

  3. Britton, M.P., 1969. Turfgrass diseases. In: A.A. Hanson & F.V. Juska (Eds). Turfgrass Science, pp. 235–288. American Society of Agronomy, Monograph No 14, Madison, WI.

  4. Delp, C.J., 1980. Coping with resistance to plant disease. Plant Dis. 64: 652–657.

    Google Scholar 

  5. Etter, A.G., 1951. How Kentucky bluegrass grows. Ann. Mo. Bot. Gard. 38: 293–375.

    Google Scholar 

  6. Evans, D.A., 1989. Somaclonal variation-genetic basis and breeding applications. Tren. Genet. 5: 46–50.

    Google Scholar 

  7. Funk, C.R. & M.K., Ahmed, 1973. Breeding grasses for turf purposes. Proc. Scott's Turfgr. Res. Conf. 4: 1–7.

    Google Scholar 

  8. Hammerschlag, F.A., 1988. Selection of peach callus for insensitivity to culture filtrates of Xanthomonas campestris pv. pruni and regeneration of resistant plants. Theor. Appl. Genet. 76: 865–869.

    Google Scholar 

  9. Jacklin, G., 1990. Seed quality-from development to use. Proc. Rutgers Turfgr. 21: 51–56.

    Google Scholar 

  10. Jorgensen, J.H., 1988. Erysiphe graminis, powdery mildew of cereals and grasses. Adv. Plant Pathol. 6: 137–157.

    Google Scholar 

  11. Kuller, W. & H. Scheinpflug, 1987. Fungal resistance to sterol biosynthesis inhibitors: a new challenge. Plant Dis. 71–1074.

  12. Larkin, P.J. & W.R., Scowcroft, 1981. Somaclonal variation — a new source of variation from cell cultures for plant improvement. Theor. Appl. Genet. 60: 197–214.

    Google Scholar 

  13. Mastebroek, H.D. & A.G., Balkema-Boomstra, 1991. Identification of growth stage dependent expression of partial resistance of barley to powdery mildew. Euphytica 58: 113–118.

    Google Scholar 

  14. Meyer, W.A., 1982. Breeding disease-resistant cool-season turfgrass cultivars for the United States. Plant Dis. 66: 341–344.

    Google Scholar 

  15. Meyer, W.A., 1985. Breeding for disease resistance in cool season turfgrasses. 26th Illinois Turfgrass Conference. Univ. of Illinois Cooperative Extension Service.

  16. Msikita, W. & H.T., Wilkinson, 1992. In vitro response of excised Kentucky bluegrass seed. In Vitr. Cellul. Dev. Biol. 28: 117 (Abstr.).

    Google Scholar 

  17. Msikita, W., 1994. Development, selection and characterization of summer patch (Magnaporthe poae Landschoot & Jackson) resistant somaclones of Kentucky bluegrass (Poa pratensis L.) genotypes. Ph.D. Thesis. University of Illinois, Urbana-Champaign, IL.

  18. Murashige, T. & F., Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 474–497.

    Google Scholar 

  19. Peschke, V.M. & R.L., Phillips, 1992. Genetic implications of somaclonal variation in plants. Adv. Genet. 30: 41–75.

    Google Scholar 

  20. Saari, E.E. & J.M., Prescott, 1975. A scale for appraising the foliar intensity of wheat diseases. Plant Dis. Repr. 59: 377–380.

    Google Scholar 

  21. SAS Institute, Inc., 1986. SAS System for Linear Models. SAS Inst., Cary, NC.

    Google Scholar 

  22. Scowcroft, W.R., P.J., Larkin & R.I.S., Brettell, 1983. Genetic variation from tissue culture. In: J.P., Helgeson & B.J., Deverall (Eds). Use of Tissue Culture and Protoplasts in Plant Pathology, pp. 139–162. Academic Press, New York.

    Google Scholar 

  23. Staba, J.E., 1969. Plant tissue culture as a technique for the phytochemist. In: M.K., Seikel & V.C., Runeckles (Eds). Recent Advances in Phytochemistry. Vol. 2, pp. 75–106. Appleton-Century Crofts, New York.

    Google Scholar 

  24. Vengris, J., 1973. Lawns. Basic Factors, Construction and Maintence of Fine Turf Areas. Thompson Publications, Fresno, CA.

    Google Scholar 

  25. Vuylsteke, D., 1988. Shoot-tip culture for the propagation, conservation and exchange of Musa germplasm. OBPGR, Rome.

    Google Scholar 

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Msikita, W., Wilkinson, H.T. Powdery mildew resistance in Kentucky bluegrass genotypes regenerated from excised seed pieces. Euphytica 78, 199–205 (1994). https://doi.org/10.1007/BF00027518

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Key words

  • apomixis
  • cultivars
  • Erysiphe graminis
  • Poa pratensis
  • tissue culture
  • vegetative propagation
  • Kentucky bluegrass
  • powdery mildew