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Toxicological effects of urban particulate matter on corneal and conjunctival epithelial cells

  • Soo-Wang Hyun
  • Su Jeong Song
  • Bongkyun Park
  • Tae Gu Lee
  • Chan-Sik KimEmail author
Original Article
  • 4 Downloads

Abstract

Exposure to urban particulate matter (UPM) is a high-risk factor for various ocular surface diseases, including dry eye syndrome. However, the effects of UPM on corneal and conjunctival epithelium damage have not been fully elucidated. In this study, we investigated the toxicological effects of UPM exposure at high concentrations by using in vitro cultures. The cell viability, mucin expression, and the secreted inflammatory mediators of corneal and conjunctival epithelial cells was observed at 24 h after exposure to UPM. The progression of cell cycle was also examined by flow cytometry at 24 h after exposure to UPM. UPM reduced cell viability in a dose-dependent manner and increased cell population in S and G2 phase. The expression of mucin-1 was attenuated by UPM exposure, but that of mucin-4 was not. UPM increased interleukin (IL)-6 release and decreased IL-8 release. The intensity of 2′,7′-dichlorofluorescein diacetate (DCF-DA) was highest at 4 h of UPM exposure. In conclusion, these results suggest that UPM causes the disruption of corneal and conjunctival epithelium by decreasing cell viability, altering cell cycle, disrupting mucin, and regulating inflammatory mediators.

Keywords

Cell cycle Cell death Corneal/conjunctival epithelial cells Mucin Urban particulate mater 

Abbreviations

CCCk-8

Cell Counting Kit-8

DES

Dry eye syndrome

DEP

Diesel exhaust particles

ELISA

Enzyme-linked immunosorbent assay

IL

Interleukin

nitro-PAHs

Nitro-substituted polycyclic aromatic hydrocarbons

PAHs

Polycyclic aromatic hydrocarbons

PCB

Polychlorinated biphenyl

ROS

Reactive oxygen species

UPM

Urban particulate matter

WHO

World Health Organization

Notes

Acknowledgements

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through High Value-added Food Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (#317033-03). This work was also supported by Korea Institute of Oriental Medicine (Project No. KSN1812080) and NST (National Research Council of Science & Technology)-KIOM (Korea Institute of Oriental Medicine) Postdoctoral Research Fellowship for Young Scientists.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Korean Society of Toxicology 2020

Authors and Affiliations

  • Soo-Wang Hyun
    • 1
    • 4
  • Su Jeong Song
    • 1
  • Bongkyun Park
    • 2
  • Tae Gu Lee
    • 2
  • Chan-Sik Kim
    • 2
    • 3
    Email author
  1. 1.Herbal Medicine Research DivisionKorea Institute of Oriental MedicineDaejeonKorea
  2. 2.Clinical Medicine DivisionKorea Institute of Oriental MedicineDaejeonKorea
  3. 3.Korean Convergence MedicineUniversity of Science Technology (UST)DaejeonKorea
  4. 4.Medicinal Evaluation Team, Bio-CenterGyeonggido Business and Science Accelerator (GBSA)SuwonKorea

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