Synthesis and Characterization of Eggshell-Derived Hydroxyapatite Bioceramics

  • Dinesh K. Patel
  • Min-Hyeok Kim
  • Ki-Taek LimEmail author
Technical Article


Several methods are frequently used to make high-value-added biomaterials from waste biomass for various biomedical applications. Hydroxyapatite (HA), a type of bio-ceramic material, has received considerable attention for use in bone tissue and implanted medical devices owing to its physiochemical properties and excellent biocompatibility. It can be synthesized from calcium- and phosphorous-containing precursor moieties such as calcium oxide and tricalcium phosphate (TCP). In this study, we synthesized an HA bio-ceramic from bio-waste eggshells via a reaction between calcium oxide and TCP, followed by heat treatment. The synthesized HA was characterized through scanning electron microscopy, X-ray diffraction, and energy-dispersive X-ray spectroscopy. The material exhibited no significant cytotoxicity even at a higher concentration, indicating high biocompatibility. Our study provides an effective way of converting low-cost bio-waste eggshells into a value-added biomaterial for tissue-engineering applications.


Bio-waste Eggshell Hydroxyapatite Bioceramics Value-added product 


Funding Information

This research was supported by the Co-operative Research Program for Agriculture Science and Technology Development (No. PJ012854012017); Rural Development Administration, Republic of Korea; Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (No. 2018R1A6A1A03025582); and the National Research Foundation of Korea (NRF-2016R1D1A3B03932921).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

42853_2019_17_MOESM1_ESM.docx (51 kb)
ESM 1 (DOCX 50 kb)


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Copyright information

© The Korean Society for Agricultural Machinery 2019

Authors and Affiliations

  1. 1.The Institute of Forest ScienceKangwon National UniversityChuncheonRepublic of Korea
  2. 2.Department of Biosystems Engineering, College of Agriculture and Life SciencesKangwon National UniversityChuncheonRepublic of Korea

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