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Oral delivery of insulin via mesoporous carbon nanoparticles for colonic release allows glycemic control in diabetic rats

  • Haorong Lin
  • Jia Zhang
  • Chaochang Yu
  • Yan Lu
  • Jie Ning
  • Sixian Le
  • Yue Li
  • Lin-quan ZangEmail author
Original Article
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Abstract

In this article, a new type of mesoporous carbon nanoparticles (MCN) was fabricated as a potential oral delivery system of insulin to reduce the adverse reactions by hypodermic injection. The mesoporous carbon nanoparticles-carried insulin (MCNI) was studied using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FT-IR) compared with the blank MCNs. The Brunauer–Emmett–Teller (BET) method was utilized to calculate the specific surface area. The pore volume and pore size distribution (PSD) curves were calculated by Barrett–Joyner–Halenda (BJH) model. The entrapment efficiency (EE%) and loading content (LC%) of insulin onto the MCNs were determined by RP-HPLC. In vitro insulin release from MCNI was determined in simulated intestinal fluid. To evaluate the pharmacodynamics of MCNIs orally, the variation of glycemia of diabetic rats after oral administration of MCNIs was compared with the rats receiving hypodermic injection of insulin. Besides, the absorption of FITC-labeled MCNs in HCT-116 cells was tested. The results showed that there is significant difference between MCNs and MCNIs through SEM, TEM, and FT-IR. The entrapment efficiency, loading content and in vitro insulin release met the requirements of the pharmacodynamic study. The specific surface area, pore volume and pore size of MCNIs were significantly decreased compared to that of MCNs. The pharmacodynamics study showed that the blood sugar level was significantly decreased after the oral administration of MCNIs. The FITC-labeled MCNs showed significant absorption in HCT-116 cells. The MCNIs were successfully synthesized with commendable entrapment efficiency and loading content which preferably decreased the blood sugar in diabetes rats via oral administration.

Keywords

Insulin Mesoporous carbon Nanoparticles Diabetes mellitus Oral administration 

Notes

Acknowledgements

This work was supported by Science and Technology Planning Project of Guangdong Province, China (no. 2016A020215157).

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interests.

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Copyright information

© Korean Carbon Society 2019

Authors and Affiliations

  • Haorong Lin
    • 1
  • Jia Zhang
    • 1
  • Chaochang Yu
    • 1
  • Yan Lu
    • 1
  • Jie Ning
    • 1
  • Sixian Le
    • 1
  • Yue Li
    • 1
  • Lin-quan Zang
    • 1
    • 2
    Email author
  1. 1.College of PharmacyGuangdong Pharmaceutical UniversityGuangzhouPeople’s Republic of China
  2. 2.Guangdong Provincial Key Laboratory for Biotechnology Drug CandidateGuangdong Pharmaceutical UniversityGuangzhouPeople’s Republic of China

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