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Inhibition of growth and ochratoxin A production in Aspergillus species by fungi isolated from coffee beans

  • Ângela Bozza de Almeida
  • Isabela Pauluk Corrêa
  • Jason Lee FuruieEmail author
  • Thiago de Farias Pires
  • Patrícia do Rocio Dalzoto
  • Ida Chapaval Pimentel
Food Microbiology - Research Paper
  • 5 Downloads

Abstract

Ochratoxin A (OTA) is a mycotoxin found in several agricultural commodities. Produced by Aspergillus spp., it is nephrotoxic and hepatotoxic and can be carcinogenic. Preventive measures are preventing fungal growth and OTA production. In this study, fungal strains (Rhizopus oryzae, Lichtheimia ramosa, Aspergillus westerdijkiae, Aspergillus niger, Aspergillus tamarii, Aspergillus sp., and Aspergillus fumigatus) isolated from coffee beans were identified for their abilities to inhibit the growth of Aspergillus ochraceus, Aspergillus westerdijkiae, Aspergillus carbonarius, and Aspergillus niger, and OTA production. All fungi strains tested were able to inhibit growth of the four Aspergillus species and OTA production, where A. niger showed the best results in both tests. L. ramosa showed the lowest growth-reducing potential, while the other fungal strains had a growth-reducing potential higher than 70% against all Aspergillus species tested. Regarding OTA production, L. ramosa and Aspergillus sp. completely inhibited the mycotoxin production by A. ochraceus and non-toxigenic strain A. niger completely inhibited OTA production by A. niger. Our findings indicate that the strains tested can be used as an alternative means to control growth of OTA-producing fungi and production of the mycotoxin in coffee beans.

Keywords

Mycotoxins Coffea arabica Aspergillus Niger 

Notes

Acknowledgments

This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). We thank the Analytical Center of the Pharmacy Department of the Federal University of Paraná for providing valuable assistance. We are also grateful to Prof. Dr. Maria Helena Fungaro of the State University of Londrina, Paraná, for kindly providing the fungal strains used as controls.

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Copyright information

© Sociedade Brasileira de Microbiologia 2019

Authors and Affiliations

  1. 1.Departamento de Patologia Básica, Laboratório de Microbiologia e Biologia MolecularUniversidade Federal do Paraná, Setor de Ciências BiológicasParanáBrazil
  2. 2.Laboratório de Genética e Cardiologia MolecularUniversidade de São Paulo, Instituto do CoraçãoSão PauloBrazil

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