Dark septate endophytic fungi increase the activity of proton pumps, efficiency of 15N recovery from ammonium sulphate, N content, and micronutrient levels in rice plants

  • Carlos Vergara
  • Karla Emanuelle Campos Araujo
  • Marcus Vinícius Loss Sperandio
  • Leandro Azevedo Santos
  • Segundo Urquiaga
  • Jerri Édson ZilliEmail author
Food Microbiology - Research Paper


Plants colonised by dark septate endophytic (DSE) fungi show increased uptake of nutrients available in the environment. The objective of the present study was to evaluate the impact of DSE fungi on the activity of proton pumps, nitrogen (N) recovery from ammonium sulphate, and nutrient accumulation in rice plants. Treatments consisted of non-inoculated plants and plants inoculated with two isolates of DSE fungi, A101 and A103. To determine N recovery from the soil, ammonium sulphate enriched with 15N was added to a non-sterile substrate while parameters associated with the activity of proton pumps and with NO3 uptake were determined in a sterile environment. The A101 and A103 fungal isolates colonised the roots of rice plants, promoting 15N uptake, growth, and accumulation of nutrients as compared with the mock control. A103 induced the expression of the plasma membrane H+-ATPase (PM H+-ATPase) isoforms OsA5 and OsA8, the activity of the PM H+-ATPase and H+-pyrophosphatase. Our results suggest that the inoculation of rice plants with DSE fungi represents a strategy to improve the N recovery from ammonium sulphate and rice plant growth through the induction of OsA5 and OsA8 isoforms and stimulation of the PM H+-ATPase and H+-pyrophosphatase.


Fertiliser-15Oryza sativa L. Pleosporales Vacuolar H+-pyrophosphatase OsA8 PM H+-ATPase 



We are indebted to the University Federal Rural do Rio de Janeiro (UFRRJ), especially for the Laboratory Nutrition of Plants, the state University of Norte Fluminense Darcy Ribeiro (UENF), especially for LBCT, the Brazilian Agricultural Research Corporation (Embrapa), for their help with the work. This work was supported by the Foundation for Support of Research in the State of Rio de Janeiro (FAPERJ), the Brazilian National Council for Scientific and Technological Development (CNPq), and the Coordination for the Improvement of Higher Education Personnel (CAPES).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Sociedade Brasileira de Microbiologia 2019

Authors and Affiliations

  • Carlos Vergara
    • 1
  • Karla Emanuelle Campos Araujo
    • 1
  • Marcus Vinícius Loss Sperandio
    • 2
  • Leandro Azevedo Santos
    • 1
  • Segundo Urquiaga
    • 3
  • Jerri Édson Zilli
    • 3
    Email author
  1. 1.Universidade Federal Rural do Rio de Janeiro, Instituto de AgronomiaSeropédicaBrazil
  2. 2.Departamento de BiologiaUniversidade Federal Rural de PernambucoRecifeBrazil
  3. 3.Embrapa AgrobiologiaSeropédicaBrazil

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