Brazilian Journal of Microbiology

, Volume 50, Issue 1, pp 223–230 | Cite as

Evaluation of the cytotoxicity and interaction of lead with lead resistant bacterium Acinetobacter junii Pb1

  • Purushottam Kumar Singh
  • Anamika Kushwaha
  • Nidhi Hans
  • Aishvarya Gautam
  • Radha RaniEmail author
Environmental Microbiology - Research Paper


This study explores the potential of lead resistant bacterium Acinetobacter junii Pb1 for adsorption/accumulation of lead using various techniques. In the present work, growth of A. junii Pb1 was investigated in the presence of a range of Pb(II) concentrations (0, 100, 250, 500, and 1000 mg l−1). Lead was found to have no toxic effect on the growth of A. junii Pb1 at 100 and 250 mg l−1 concentrations. However, further increase in Pb(II) concentration (500 mg l−1) showed increase in lag phase, though growth remained unaffected and significant growth inhibition was observed when concentration was increased to 1000 mg l−1. Same was confirmed by the observations of flow cytometry. Further, the effect of Pb(II) on A. junii Pb1 was evaluated by using fluorescence microscopy, spectrofluorimetry, and flow cytometry. The spectrofluorimetry and fluorescence microscopy results revealed the accumulation of Pb(II) inside the bacterial cells as evident by green fluorescence due to lead binding fluorescent probe, Leadmium Green AM dye. Flow cytometry observations indicate an increase in cell size and granularity of exposure to lead. Thus, present work provides a new understanding of Pb(II) tolerance in A. junii Pb1 and its potential use in remediation of lead from contaminated soil.


Lead Acinetobacter junii Cytotoxicity Propidium iodide Leadmium Green AM dye 



The authors are grateful to MNNIT, Allahabad for this research. The authors are grateful to CIR, MNNIT, Allahabad and CMDR, MNNIT, Allahabad for providing facility for spectrofluorimetry and flow cytometry respectively. The authors are grateful to Dr. Jyotsna Sinha for the proofread for English language and grammar.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Sociedade Brasileira de Microbiologia 2019

Authors and Affiliations

  • Purushottam Kumar Singh
    • 1
  • Anamika Kushwaha
    • 1
  • Nidhi Hans
    • 1
  • Aishvarya Gautam
    • 1
  • Radha Rani
    • 1
    Email author
  1. 1.Department of BiotechnologyMotilal Nehru National Institute of TechnologyAllahabadIndia

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