Brazilian Journal of Microbiology

, Volume 50, Issue 1, pp 79–84 | Cite as

Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol

  • Xiuwen Wang
  • Xiaohua Zhang
  • Qingshou Yao
  • Dongliang Hua
  • Jiayang QinEmail author
Biotechnology and Industrial Microbiology - Research Paper


Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Comparative proteomic analyses were conducted on the strain H. roseonigra ATCC 20624 by using sclareol or glucose as the sole carbon source. A total of 79 upregulated protein spots with a > 2.0-fold difference in abundance on 2-D gels under sclareol stress conditions were collected for further identification. Seventy spots were successfully identified and finally integrated into 30 proteins. The upregulated proteins under sclareol stress are involved in carbon metabolism and nitrogen metabolism, and replication, transcription, and translation processes. Eighteen upregulated spots were identified as aldehyde dehydrogenases, which indicating that aldehyde dehydrogenases might play an important role in sclareol metabolism. Overall, this study may lay the fundamentals for further cell engineering to improve sclareol glycol production.


Comparative proteomic Hyphozyma roseonigra Sclareol Sclareol glycol Ambroxide 


Funding information

This work was supported by the National Natural Science Foundation of China (31200078), the Shandong Province Science and Technology Project (No. ZR2017MC023 and 2015GSF117034), and the Jinan Youth Science and Technology Star Project (No. 201406021).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

42770_2019_40_MOESM1_ESM.docx (23 kb)
Table S1 Relative abundance of the 117 differentially displayed protein spots. (DOCX 23 kb)
42770_2019_40_Fig4_ESM.png (361 kb)
Figure S1

Chromatogram of sclareol conversion after 12 h cultivation of H. roseonigra ATCC 20624. (PNG 361 kb)

42770_2019_40_MOESM2_ESM.tif (409 kb)
High resolution figure (TIF 408 kb)


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Copyright information

© Sociedade Brasileira de Microbiologia 2019

Authors and Affiliations

  • Xiuwen Wang
    • 1
  • Xiaohua Zhang
    • 1
  • Qingshou Yao
    • 1
  • Dongliang Hua
    • 2
  • Jiayang Qin
    • 1
    Email author
  1. 1.College of Pharmacy, Binzhou Medical UniversityYantaiPeople’s Republic of China
  2. 2.Key Laboratory for Biomass Gasification Technology of Shandong ProvinceEnergy Research Institute of Shandong Academy of SciencesJinanChina

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