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Brazilian Journal of Microbiology

, Volume 50, Issue 1, pp 133–137 | Cite as

The occurrence of polyomaviruses WUPyV and KIPyV among patients with severe respiratory infections

  • Débora Bellini CaldeiraEmail author
  • Luciano Kleber de Souza Luna
  • Aripuana Watanabe
  • Ana Helena Perosa
  • Celso Granato
  • Nancy Bellei
Clinical Microbiology - Research Paper

Abstract

In 2007, the new polyomaviruses WUPyV and KIPyV were identified in patients with acute respiratory infections. The aim of this study was to investigate these viruses in hospitalized patients with severe acute respiratory infection (SARI). A retrospective study was conducted with 251 patients, from April 2009 to November 2010, using nasopharyngeal aspirates, naso- and oropharyngeal swab samples from hospitalized patients (children < 12 years and adults) who had SARI within 7 days of the onset of symptoms, including fever (> 38.8 °C), dyspnea, and cough. Clinical and epidemiological information was obtained through standardized questionnaire. Enrolled patients were initially suspected to have influenza A(H1N1)pdm09 infections. WUPyV and KIPyV were detected by real-time PCR. Samples were also tested for influenza A and B viruses, human respiratory syncytial virus, rhinovirus, metapneumovirus, coronavirus, adenovirus, and parainfluenza viruses. WUPyV and KIPyV were detected in 6.77% (4.78% and 1.99%, respectively) of hospitalized patients with SARI. All samples from children showed coinfections (rhinovirus was the most commonly detected). Six adults had polyomavirus infection and four (1.6%) had monoinfection. Of them, 3 reported comorbidities including immunosuppression and 1 patient had worse outcome, requiring ICU admission. These preliminary data may suggest a possible role of polyomaviruses in SARI among immunocompromised adult patients.

Keywords

Severe acute respiratory infection Polyomaviruses WUPyV KIPyV Real-time PCR 

Notes

Funding information

This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo – FAPESP [grant number 2016/09279-6.]. DBC e LKSL são bolsistas da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brasil.

Compliance with ethical standards

This study was approved by the Ethics Committee of Sao Paulo Federal University (CEP 0090/2016).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Sociedade Brasileira de Microbiologia 2018

Authors and Affiliations

  • Débora Bellini Caldeira
    • 1
    Email author
  • Luciano Kleber de Souza Luna
    • 1
  • Aripuana Watanabe
    • 2
  • Ana Helena Perosa
    • 1
  • Celso Granato
    • 1
  • Nancy Bellei
    • 1
  1. 1.Medicine Department, Clinical Virology Laboratory, Infectious Diseases UnitSao Paulo Federal UniversitySao PauloBrazil
  2. 2.Department of parasitology, microbiology and immunology, Biologic Sciences InstituteFederal University of Juiz de ForaMinas GeraisBrazil

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