Advertisement

Brazilian Journal of Microbiology

, Volume 50, Issue 1, pp 329–333 | Cite as

Isolation and genome characterization of canine parvovirus type 2c in Brazil

  • Felipe Wolf Jaune
  • Isis Indaiara Gonçalves Granjeiro Taques
  • Jackeliny dos Santos Costa
  • João Pessoa AraújoJr
  • Márcia H. B. Catroxo
  • Luciano Nakazato
  • Daniel Moura de AguiarEmail author
Veterinary Microbiology - Short Communication
  • 3 Downloads

Abstract

This study focused on the isolation and characterization of parvovirus in an infected dog in midwestern Brazil. Non-enveloped icosahedral parvovirus-like particles were isolated in CRFK cells and were allocated to a clade comprised of strains of CPV-2c, based on genome analysis. This is the first isolate of CPV-2c genomically characterized in Brazil.

Keywords

Transmission electron microscopy Diarrhea Culture Dogs 

Notes

Acknowledgements

We gratefully acknowledge the following Brazilian research funding agencies: FAPEMAT (Research Support Foundation of the State of Mato Grosso) for its financial support of this work (under process no. 570083/2008) and CAPES (Federal Agency for the Support and Improvement of Higher Education) and CNPq (National Council for Scientific and Technological Development) for scholarships awarded to I.I.G.G. Taques and for Scientific Productivity Grants awarded to L. Nakazato, J.P. Araújo Jr., and D.M. Aguiar.

Compliance with ethical standards

This study was approved by the Animal Ethics Committee of the Federal University of Mato Grosso under the protocol 23108.031550/09-02.

References

  1. 1.
    Appel MJG, Cooper BJ, Greisen H, Scott F, Carmichael LE (1979) Canine viral enteritis. I. Status report on corona- and parvo-like viral enteritidis. Cornell Vet 69:123–133Google Scholar
  2. 2.
    Kelly WR (1978) An enteric disease of dogs resembling feline panleukopenia. Aust Vet J 54:593CrossRefGoogle Scholar
  3. 3.
    Dezengrini R, Weiblen R, Flores EF (2007) Soroprevalência das infecções por parvovírus, adenovírus, coronavírus canino e pelo vírus da cinomose em cães de Santa Maria, Rio Grande do Sul, Brasil. Ciência Rural 37:183–189CrossRefGoogle Scholar
  4. 4.
    Pinto LD, Streck AF, Gonçalves KR, Souza CK, Corbellini AO, Corbellini LG, Canal CW (2012) Typing of canine parvovirus strains circulating in Brazil between 2008 and 2010. Virus Res 165:29–33CrossRefGoogle Scholar
  5. 5.
    Fontana DS, Rocha PRD, Cruz RAS, Lopes LL, Melo ALT, Silveira MM, Aguiar DM, Pescador CA (2013) Phylogenetic study of canine parvovirus type 2c in midwestern Brazil. Pesq Vet Bras. 33:214–218CrossRefGoogle Scholar
  6. 6.
    Oliveira PSB, Cargnelutti JF, Masuda EK, Fighera RA, Kommers GD, Silva MC, Weiblen R, Flores EF (2018) Epidemiological, clinical and pathological features of canine parvovirus 2c infection in dogs from southern Brazil. Pesq Vet Bras 38:113–118CrossRefGoogle Scholar
  7. 7.
    Cotmore SF, Agbandje-McKenna M, Chiorini JA, Mukha DV, Pintel DJ, Qiu J, Soderlund-Venermo M, Tattersall P, Tijssen P, Gatherer D, Davison AJ (2014) The family Parvoviridae. Arch Virol 159:1239–1247CrossRefGoogle Scholar
  8. 8.
    Reed AP, Jones EV, Miller TJ (1988) Nucleotide sequence and genome organization of canine parvovirus. J Virol 62:266–276Google Scholar
  9. 9.
    Mohan Raj J, Mukhopadhyay HK, Thanislass J, Antony PX, Pillai RM (2010) Isolation, molecular characterization and phylogenetic analysis of canine parvovirus. Infect Genet and Evol 10:1237–1241CrossRefGoogle Scholar
  10. 10.
    Han SC, Guo HC, Sun SQ, Shu L, Wei YQ, Sun DH, Cao SZ, Peng GN, Liu XT (2015) Full-length genomic characterizations of two canine parvoviroses prevalent in northwest China. Arch Microbiol 197:621–626CrossRefGoogle Scholar
  11. 11.
    Parrish CR, Aquadro CF, Strassheim ML, Evermann JF, Sgro JY, Mohammed HO (1991) Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus. J Virol 65:6544–6552Google Scholar
  12. 12.
    Parrish CR, O’Connell PH, Evermann JF, Carmichel LE (1985) Natural variation of canine parvovirus. Science 230:1046–1048CrossRefGoogle Scholar
  13. 13.
    Zhou P, Zeng W, Zhang X, Shoujun L (2017) The genetic evolution of canine parvovirus – a new perspective. PLoS One 12:e0175035CrossRefGoogle Scholar
  14. 14.
    Buonavoglia C, Martella V, Pratelli A, Tempesta M, Cavalli A, Buonavoglia C, Bozzo G, Elia G, Decaro N, Carmichael LE (2001) Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol. 82:3021–3025CrossRefGoogle Scholar
  15. 15.
    Decaro N, Buonavoglia C (2012) Canine parvovirus - a review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet Microbiol 155:1–12CrossRefGoogle Scholar
  16. 16.
    Soma T, Taharaguchi S, Ohinata T, Ishii H, Hara M (2013) Analysis of the VP2 protein gene of canine parvovirus strains from affected dogs in Japan. Res Vet Sci 94:368–371CrossRefGoogle Scholar
  17. 17.
    Streck AF, Souza CK, Gonçalves KR, Zang L, Pinto LD, Canal CW (2009) First detection of canine parvovirus type 2c in Brazil. Braz J Microbiol 40:465–469CrossRefGoogle Scholar
  18. 18.
    Strottmann DM, Scortegagna G, Kreutz LC, Barcellos LJG, Frandoloso R, Anziliero D (2008) Diagnóstico e estudo sorológico da infecção pelo parvovírus canino em cães de Passo Fundo, Rio Grande do Sul, Brasil. Ciência Rural 38:400–405CrossRefGoogle Scholar
  19. 19.
    Hirayama K, Kano R, Hosokawa-Kanai T, Tuchiya K, Tsuyama S, Nakamura Y, Sasaki Y, Hasegawa A (2005) VP2 gene of a canine parvovirus isolate from stool of a puppy. J Vet Med Sci 67:139–143CrossRefGoogle Scholar
  20. 20.
    Brenner S, Horne RW (1959) A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta 34:103–110CrossRefGoogle Scholar
  21. 21.
    Hayat MA, Miller SE (1990) Negative staining. Mc. Graw-Hill Publ. Company, New YorkGoogle Scholar
  22. 22.
    Martins AMCRPF, Bersano JG, Ogata R, Amante G, Nastari BDB, Catroxo MHB (2013) Diagnosis to detect porcine transmissible gastroenteritis virus (TGEV) by optical and transmission electron microscopy techniques. Int J Morphol 31:706–715CrossRefGoogle Scholar
  23. 23.
    Ullmann LS, Tozato CC, Malossi CD, Cruz TF, Cavalcante RV, Kurissio JK, Cagnini DQ, Rodrigues MV, Biondo AW, Araujo JP Jr (2015) Comparative clinical sample preparation of DNA and RNA viral nucleic acids for a commercial deep sequencing system (Illumina MiSeq(®)). J Virol Methods 220:60–63CrossRefGoogle Scholar
  24. 24.
    Mochizuki M, San Gabriel MC, Nakatani H, Yoshida M, Harasawa R (1993) Comparison of polymerase chain reaction with virus isolation and haemagglutination assays for the detection of canine parvoviruses in faecal specimens. Res Vet Sci 55:60–63CrossRefGoogle Scholar
  25. 25.
    Bourtonboy G, Coignoul F, Delferriere N, Pastoret PP (1979) Canine hemorrhagic enteritis: detection of viral particles by electron microscopy. Arch Virol 61:1–11CrossRefGoogle Scholar
  26. 26.
    Amo AN, Aprea AN, Petruccelli MA (1999) Detection of viral particles in feces of young dogs and their relationship with clinical signs. Rev Microbiol 30:237–241CrossRefGoogle Scholar
  27. 27.
    Truyen U (2006) Evolution of canine parvovirus--a need for new vaccines? Vet Microbiol 117:9–13CrossRefGoogle Scholar
  28. 28.
    Pereira CAD, Leal ES, Durigon EL (2007) Selective regimen shift and demographic grow increase associated with the emergence of high-fitness variants of canine parvovirus. Infect Genet Evol 7:399–409CrossRefGoogle Scholar
  29. 29.
    Zhang R, Yang S, Zhang W, Zhang T, Xie Z, Feng H, Wang S, Xia X (2010) Phylogenetic analysis of the VP2 gene of canine parvoviruses circulating in China. Virus Genes 40:397–402CrossRefGoogle Scholar
  30. 30.
    Hoelzer K, Shackelton LA, Parrish CR, Holmes EC (2008) Phylogenetic analysis reveals the emergence, evolution and dispersal of carnivore parvoviruses. J Gen Virol 89:2280–2289CrossRefGoogle Scholar

Copyright information

© Sociedade Brasileira de Microbiologia 2018

Authors and Affiliations

  • Felipe Wolf Jaune
    • 1
  • Isis Indaiara Gonçalves Granjeiro Taques
    • 1
    • 2
  • Jackeliny dos Santos Costa
    • 1
    • 2
  • João Pessoa AraújoJr
    • 3
  • Márcia H. B. Catroxo
    • 4
  • Luciano Nakazato
    • 5
  • Daniel Moura de Aguiar
    • 2
    • 6
    Email author
  1. 1.Postgraduate Program in Veterinary Science, Faculty of Veterinary Medicine (FAVET)Federal University of Mato Grosso (UFMT)CuiabáBrazil
  2. 2.Laboratory of Virology and Rickettsiosis, Veterinary Hospital (HOVET), Faculty of Veterinary Medicine (FAVET)Federal University of Mato Grosso (UFMT)CuiabáBrazil
  3. 3.Laboratory of Molecular Diagnostics, Institute of Biotechnology (IBTEC)São Paulo State University (UNESP)BotucatuBrazil
  4. 4.Laboratory of Electron MicroscopyBiological Institute (IB)São PauloBrazil
  5. 5.Laboratory of Molecular Biology, Veterinary Hospital (HOVET), Faculty of Veterinary Medicine (FAVET)Federal University of Mato Grosso (UFMT)CuiabáBrazil
  6. 6.Laboratório de Virologia e RickettsiosesHospital Veterinário da Universidade Federal de Mato GrossoCuiabáBrazil

Personalised recommendations