Brazilian Journal of Microbiology

, Volume 50, Issue 1, pp 53–65 | Cite as

A new strain of Aspergillus tubingensis for high-activity pectinase production

  • Danmei Huang
  • Yuyang Song
  • Yanlin Liu
  • Yi QinEmail author
Biotechnology and Industrial Microbiology - Research Paper


Pectinase is a general term for a class of enzymes that decompose pectin. To obtain a fungal strain with high-activity pectinase of potential commercial importance, we screened microorganisms from the soil of vineyards, performed mutation breeding by ultraviolet (UV) and nitrosoguanidine (NTG) mutagenesis, and performed comparisons to commercially available pectinases. We found that the derived pectinase-producing strain Rn14-88A had the highest pectinase activity of 8363.215 U/mL, and identified it using internal transcribed spacer sequence analysis as Aspergillus tubingensis. Rn14-88A was the original strain for UV mutagenesis, from which mutant strain R-7-2-4 had the highest pectinase enzyme activity (9198.68 U/mL), which was a 9.99% increase compared to that of Rn14-88A. Following NTG mutagenesis of R-7-2-4, mutant strain Y1-3-2-6 had a pectinase enzyme activity of 9843.34 U/mL, which reflects a 6.36% increase compared to the pectinase activity of R-7-2-4. Subsequently, another round of NTG mutagenesis was performed on Y1-3-2-6, and the mutagenic strain Y2-6-3-4 exhibited an improved enzyme activity of 21,864.34 U/mL, which was 161.44% higher than that of Rn14-88A. Through liquid fermentation experiments of A. tubingensis Y2-6-3-4, it was determined that pectinase activity was the highest at a fermentation time of 20 h. Therefore, we conclude that A. tubingensis Y2-6-3-4 has potential for use in commercial production.


Screening Pectinase Aspergillus tubingensis Ultraviolet mutagenesis (UV) Nitrosoguanidine (NTG) mutagenesis 


Funding information

The study was funded by the National Key R&D Program of China (2016YFD0400504-01), Viticulture Experiment Station scientific and technological transformative project of Northwest A&F University (TGZX2017-23), Scientific and Technological Transformative Special Project of Shanxi Province (201604D132034), and China Agriculture Research System (grant no. CARS-29-jg-3).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Sociedade Brasileira de Microbiologia 2019

Authors and Affiliations

  • Danmei Huang
    • 1
  • Yuyang Song
    • 1
    • 2
  • Yanlin Liu
    • 1
    • 2
  • Yi Qin
    • 1
    • 2
    Email author
  1. 1.College of EnologyNorthwest A&F UniversityYanglingChina
  2. 2.Shaanxi Engineering Research Center for Viti-VinicultureYanglingChina

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