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A protocol for selection of Trichoderma spp. to protect grapevine pruning wounds against Lasiodiplodia theobromae

  • Renata Marraschi
  • Ana Beatriz Monteiro Ferreira
  • Roselaine Nunes da Silva Bueno
  • Juliana A. B. P. Leite
  • Cleusa Maria Mantovanello Lucon
  • Ricardo Harakava
  • Luís Garrigós Leite
  • Carlos Roberto Padovani
  • César Júnior Bueno
Environmental Microbiology - Research Paper
  • 2 Downloads

Abstract

There is no standardized protocol to select Trichoderma against trunk disease pathogens in grapevine. This study describes a protocol to select Trichoderma isolates for protecting the pruning wounds of grapevine against Lasiodiplodia theobromae. Thus, four assays were performed: (1) in vitro antagonism of Trichoderma isolates, molecularly identified, to control L. theobromae; (2) in vitro effect of grapevine fungicides on the conidia of the antagonists; (3) capability of Trichoderma to survive and endophytically penetrate in grapevine canes; and (4) efficiency of Trichoderma and fungicides to protect the pruning wounds of grapevine shoots against L. theobromae. T. asperelloides (02/03), T. koningiopsis (09/02), and T. asperellum (01/13) were the best antagonists for L. theobromae. The conidia of the three isolates were affected only by tebuconazole, with the first two fungi being the most tolerant to the fungicides. The Trichoderma isolates survived on the cane tip for 15 days, but only T. asperelloides (02/03) penetrated endophytically 4 cm in the cane and showed preventive and curative capability to control L. theobromae, with similar efficiency as that of the fungicides in both the periods tested post inoculation. These four assays are suggested to select Trichoderma against L. theobromae or other trunk disease pathogens.

Keywords

Biocontrol Fungicides Niagara Rosada Decline 

Notes

Acknowledgements

The authors thank Mr. Daniel Fernando Miqueletto, Agricultural Engineer of City Council of Louveira city, São Paulo State (SP), Brazil, for the financial support given to the project (FUNDAG 1018–IB-PORTA ENXERTOS). The authors would like to thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior CAPES - Brazil (Finance Code 001) for supporting the first author.

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Copyright information

© Sociedade Brasileira de Microbiologia 2018

Authors and Affiliations

  • Renata Marraschi
    • 1
  • Ana Beatriz Monteiro Ferreira
    • 1
  • Roselaine Nunes da Silva Bueno
    • 1
  • Juliana A. B. P. Leite
    • 1
  • Cleusa Maria Mantovanello Lucon
    • 2
  • Ricardo Harakava
    • 2
  • Luís Garrigós Leite
    • 1
  • Carlos Roberto Padovani
    • 3
  • César Júnior Bueno
    • 1
  1. 1.Instituto BiológicoCampinasBrazil
  2. 2.Instituto BiológicoAvenida Conselheiro Rodrigues Alves, São PauloBrazil
  3. 3.UNESP , Rua Prof. Dr. Antônio Celso Wagner ZaninBotucatuBrazil

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