Gene transcription profiling of Aspergillus oryzae 3.042 treated with ergosterol biosynthesis inhibitors
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Ergosterol, a unique component of fungal cells, is not only important for fungal growth and stress responses but also holds great economic value. Limited studies have been performed on ergosterol biosynthesis in Aspergillus oryzae, a safe filamentous fungus that has been used for the manufacture of oriental fermented foods. This study revealed that the ergosterol biosynthesis pathway is conserved between Saccharomyces cerevisiae and A. oryzae 3.042 by treatment with ergosterol biosynthesis inhibitors and bioinformatics analysis. However, the ergosterol biosynthesis pathway in A. oryzae 3.042 is more complicated than that in S. cerevisiae as there are multiple paralogs encoding the same biosynthetic enzymes. Using RNA-seq, this study identified 138 and 104 differentially expressed genes (DEG) in response to the ergosterol biosynthesis inhibitors tebuconazole and terbinafine, respectively. The results showed that the most common DEGs were transport- and metabolism-related genes. There were only 17 DEGs regulated by both tebuconazole and terbinafine treatments and there were 256 DEGs between tebuconazole and terbinafine treatments. These results provide new information on A. oryzae ergosterol biosynthesis and regulation mechanisms, which may lay the foundation for genetic modification of the ergosterol biosynthesis pathway in A. oryzae.
KeywordsAspergillus oryzae Ergosterol biosynthesis Inhibitors Transcriptome
This study was financially supported by the National Natural Science Foundation of China (NSFC) (Grant nos. 31700068 and 31460447), International S&T Cooperation Project of Jiangxi Provincial (Grant no. 20142BDH80003), General Science and Technology Project of Nanchang City (Grant no. 3000035402), “555 Talent Project” of Jiangxi Province, Natural Science Foundation of Jiangxi Province (Grant nos. 20181BAB214001 and 20171BAB214004), and the Open Foundation of Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization (Grant no. EWPL201705).
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Conflict of interest
The authors declare that they have no conflict of interest.
- 6.Whiteman MK, Jeng G, Samarina A, Akatova N, Martirosyan M, Kissin DM, Curtis KM, Marchbanks PA, Hillis SD, Mandel MG, Jamieson DJ (Jan 2016) Associations of hormonal contraceptive use with measures of HIV disease progression and antiretroviral therapy effectiveness. Contraception 93(1):17–24CrossRefPubMedGoogle Scholar
- 7.Hu Z, He B, Ma L, Sun Y, Niu Y, Zeng B (2017) Recent advances in ergosterol biosynthesis and regulation mechanisms in Saccharomyces cerevisiae Google Scholar
- 17.Machida M, Asai K, Sano M, Tanaka T, Kumagai T, Terai G, Kusumoto KI, Arima T, Akita O, Kashiwagi Y, Abe K, Gomi K, Horiuchi H, Kitamoto K, Kobayashi T, Takeuchi M, Denning DW, Galagan JE, Nierman WC, Yu J, Archer DB, Bennett JW, Bhatnagar D, Cleveland TE, Fedorova ND, Gotoh O, Horikawa H, Hosoyama A, Ichinomiya M, Igarashi R, Iwashita K, Juvvadi PR, Kato M, Kato Y, Kin T, Kokubun A, Maeda H, Maeyama N, Maruyama JI, Nagasaki H, Nakajima T, Oda K, Okada K, Paulsen I, Sakamoto K, Sawano T, Takahashi M, Takase K, Terabayashi Y, Wortman JR, Yamada O, Yamagata Y, Anazawa H, Hata Y, Koide Y, Komori T, Koyama Y, Minetoki T, Suharnan S, Tanaka A, Isono K, Kuhara S, Ogasawara N, Kikuchi H (2005) Genome sequencing and analysis of Aspergillus oryzae. Nature 438(7071):1157–1161CrossRefGoogle Scholar
- 19.Bhattacharya S, Esquivel BD, White TC (2018) Overexpression or deletion of ergosterol biosynthesis genes alters doubling time, response to stress agents and drug susceptibility in Saccharomyces cerevisiae, mBio 9(4):e01291–18Google Scholar
- 24.Mikeš V, Lochman J, Kašparovský T (2006) Ergosterol is a signal molecule that leads to the expression of defence-related genes in tobaccoGoogle Scholar