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Gene transcription profiling of Aspergillus oryzae 3.042 treated with ergosterol biosynthesis inhibitors

  • Zhihong Hu
  • Ganghua Li
  • Yunlong Sun
  • Yali Niu
  • Long Ma
  • Bin He
  • Mingqiang Ai
  • Jizhong Han
  • Bin Zeng
Biotechnology and Industrial Microbiology - Research Paper
  • 4 Downloads

Abstract

Ergosterol, a unique component of fungal cells, is not only important for fungal growth and stress responses but also holds great economic value. Limited studies have been performed on ergosterol biosynthesis in Aspergillus oryzae, a safe filamentous fungus that has been used for the manufacture of oriental fermented foods. This study revealed that the ergosterol biosynthesis pathway is conserved between Saccharomyces cerevisiae and A. oryzae 3.042 by treatment with ergosterol biosynthesis inhibitors and bioinformatics analysis. However, the ergosterol biosynthesis pathway in A. oryzae 3.042 is more complicated than that in S. cerevisiae as there are multiple paralogs encoding the same biosynthetic enzymes. Using RNA-seq, this study identified 138 and 104 differentially expressed genes (DEG) in response to the ergosterol biosynthesis inhibitors tebuconazole and terbinafine, respectively. The results showed that the most common DEGs were transport- and metabolism-related genes. There were only 17 DEGs regulated by both tebuconazole and terbinafine treatments and there were 256 DEGs between tebuconazole and terbinafine treatments. These results provide new information on A. oryzae ergosterol biosynthesis and regulation mechanisms, which may lay the foundation for genetic modification of the ergosterol biosynthesis pathway in A. oryzae.

Keywords

Aspergillus oryzae Ergosterol biosynthesis Inhibitors Transcriptome 

Notes

Acknowledgments

This study was financially supported by the National Natural Science Foundation of China (NSFC) (Grant nos. 31700068 and 31460447), International S&T Cooperation Project of Jiangxi Provincial (Grant no. 20142BDH80003), General Science and Technology Project of Nanchang City (Grant no. 3000035402), “555 Talent Project” of Jiangxi Province, Natural Science Foundation of Jiangxi Province (Grant nos. 20181BAB214001 and 20171BAB214004), and the Open Foundation of Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization (Grant no. EWPL201705).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Sociedade Brasileira de Microbiologia 2018

Authors and Affiliations

  • Zhihong Hu
    • 1
  • Ganghua Li
    • 2
  • Yunlong Sun
    • 1
  • Yali Niu
    • 1
  • Long Ma
    • 1
  • Bin He
    • 1
  • Mingqiang Ai
    • 1
  • Jizhong Han
    • 1
  • Bin Zeng
    • 1
  1. 1.Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-vitro Diagnostic Reagents and Devices of Jiangxi Province, college of life sciencesJiangxi Science & Technology Normal UniversityNanchangChina
  2. 2.Hubei Key Laboratory of Edible Wild Plants Conservation and UtilizationHubei Normal UniversityHuangshiChina

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