Evaluation on the efficacy and immunogenicity of recombinant DNA plasmids expressing S gene from porcine epidemic diarrhea virus and VP7 gene from porcine rotavirus
Porcine rotavirus (PoRV) and porcine epidemic diarrhea virus (PEDV) usually co-infect pigs in modern large-scale piggery, which both can cause severe diarrhea in newborn piglets and lead to significant economic losses to the pig industry. The VP7 protein is the main coat protein of PoRV, and the S protein is the main structural protein of PEDV, which are capable of inducing neutralizing antibodies in vivo. In this study, a DNA vaccine pPI-2.EGFP.VP7.S co-expressing VP7 protein of PoRV and S protein of PEDV was constructed. Six 8-week-old mice were immunized with the recombinant plasmid pPI-2.EGFP.VP7.S. The high humoral immune responses (virus specific antibody) and cellular immune responses (IFN-γ, IL-4, and spleen lymphocyte proliferation) were evaluated. The immune effect through intramuscular injection increased with plasmid dose when compared with subcutaneous injection. The immune-enhancing effect of IFN-α adjuvant was excellent compared with pig spleen transfer factor and IL-12 adjuvant. These results demonstrated that pPI-2.EGFP.VP7.S possess the immunological functions of the VP7 proteins of PoRV and S proteins of PEDV, indicating that pPI-2.EGFP.VP7.S is a candidate vaccine for porcine rotaviral infection (PoR) and porcine epidemic diarrhea (PED).
KeywordsPoRV PEDV Co-expression DNA vaccine Immune effect
This work was supported by the Science and technology program of Sichuan (Project No. 2014NZ0043) (Project No. 2017NZ0038), Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period (2015BAD12B04-2.3), and Sichuan Crops and Animals Breeding Special Project during the Thirteenth Five-year Plan Period (2016NYZ0052).
Compliance with ethical standards
The authors declare that they have no competing interests.
- 1.Belperron AA, Feltquate D, Fox BA, Horii T, Bzik DJ (1999) Immune responses induced by gene gun or intramuscular injection of DNA vaccines that express immunogenic regions of the serine repeat antigen from Plasmodium falciparum. Infect Immun 67:5163–5169Google Scholar
- 2.Chen HW, Pan CH, Liau MY, Jou R, Tsai CJ, Wu HJ, Lin YL, Tao MH (1999) Screening of protective antigens of Japanese encephalitis virus by DNA immunization: a comparative study with conventional viral vaccines. J Virol 73:10137–10145Google Scholar
- 3.Cheng G, Zhao X, Yan W, Wang W, Zuo X, Huang K, Liu Y, Chen J, Wang J, Cong W (2007) Alpha interferon is a powerful adjuvant for a recombinant protein vaccine against foot-and-mouth disease virus in swine, and an effective stimulus of in vivo immune response. Vaccine 25:5199–5208CrossRefGoogle Scholar
- 4.Gabbay YB, † AAB, Oliveira DS, Linhares AC, Mascarenhas JDP, Barardi CRM, Simões CMO, Wang Y, Glass RI, and Jiang B. Evidence for zoonotic transmission of group C rotaviruses among children in Belém, Brazil. J Med Virol 2008, 80, 1666–1674Google Scholar
- 8.Huang KY, Yang GL, Jin YB, Liu J, Chen HL, Wang PB, Jiang YL, Shi CW, Huang HB, Wang JZ (2017) Construction and immunogenicity analysis of lactobacillus plantarum expressing a porcine epidemic diarrhea virus S gene fused to a DC-targeting peptide. Virus ResGoogle Scholar
- 10.Kim D, Martinezsobrido L, Choi C, Petroff N, Garcíasastre A, Niewiesk S, Carsillo T (2011) Induction of type I interferon secretion through recombinant Newcastle disease virus expressing measles virus hemagglutinin stimulates antibody secretion in the presence of maternal antibodies. J Virol 85:200–207CrossRefGoogle Scholar
- 18.Suocheng W, Tuanjie C, Changjun S, Fengling T, Zhongren M (2015) Subunit vaccine preparation of bovine rotavirus and its efficacy in mice. Iran J Immunol 12:188Google Scholar
- 21.Yang WY, Zhu L, Zhou YC, Guo WZ, Zhi-Wen XU, Center AB, University SA (2014) Isolation and identification of porcine rotavirus in Sichuan province and its proliferation profile detected by fluorescence quantitative PCR. Chin J Vet Sci 34:192–198Google Scholar
- 23.Zhang Y, Zhang X, Liao X, Huang X, Cao S, Wen X, Wen Y, Wu R, Liu W (2016) Construction of a bivalent DNA vaccine co-expressing S genes of transmissible gastroenteritis virus and porcine epidemic diarrhea virus delivered by attenuated Salmonella typhimurium. Virus Genes 52:354–364CrossRefGoogle Scholar
- 25.Zhao Z, Liu HL, Fan GU, Zhou TF, Qin JJ, Zhi-Wen XU, Center AB, and University SA (2015) Part characteristics of porcine epidemic diarrhea virus SC-P strain. Chin J Vet SciGoogle Scholar