Brazilian Journal of Microbiology

, Volume 50, Issue 1, pp 13–22 | Cite as

Identification of key genes and signaling pathways during Sendai virus infection in vitro

  • Wenqiang WeiEmail author
  • Wanting Kong
Bacterial, Fungal and Virus Molecular Biology - Research Paper


Sendai virus (SeV) has been used as a model strain to reveal molecular features of paramyxovirus biology. In this study, we comprehensively analyzed the gene profiling of murine macrophages and airway epithelial cells in response to SeV using gene expression data. The significantly differentially expressed genes (DEGs) were screened by GEO2R. Gene ontology (GO) and pathway enrichment analyses were performed by DAVID. The protein-protein interaction (PPI) map of DEGs was constructed by STRING. The modules of PPI network are produced by molecular complex detection (MCODE) plug-in of Cytoscape. In total, 241 up- and 83 downregulated DEGs were identified in airway epithelial cells while 130 up- and 148 downregulated in macrophage. Particularly, Tmem119 and Colla2 are significantly downregulated in airway epithelial cells and macrophages, respectively. Functional enrichment analysis showed that upregulated DEGs are clustered in innate immunity and inflammatory response in both cell types, whereas downregulated DEGs are involved in host metabolic pathway in airway epithelial cells. PI3K-AKT signaling pathway is downregulated in macrophages. PPI network analysis indicated that some high degree of nodes exist in both cell types, such as Stat1, Tnf, and Cxcl10. In conclusion, SeV infection can induce different host cell responses in airway epithelial cells and macrophages.


SeV Differentially expressed genes Immunity Metabolism Signaling pathway 


Funding information

This work was supported by a grant from the China Scholarship Council (201608410250), National Natural Science Foundation of China (31501701), and the Plant Foundation for Young Scientists of Henan University (CX0000A40557).

Compliance with ethical standards

Conflict of interest

The authors declared that they have no conflict of interest.

Supplementary material

42770_2018_21_MOESM1_ESM.tif (229 kb)
ESM 1 Functional annotation of down-regulated differentially expressed genes (DEGs) with fold change>=2.0 in SeV- infected airway epithelial cells. The x-axis represents the number of DEGs, and y-axis represents the GO categories. (TIF 228 kb)
42770_2018_21_MOESM2_ESM.tif (245 kb)
ESM 2 Functional annotation of down-regulated differentially expressed genes (DEGs) with fold change>=2.0 in SeV-infected macrophages. The x-axis represents the number of DEGs, and y-axis represents the GO categories. (TIF 245 kb)
42770_2018_21_MOESM3_ESM.tif (211 kb)
ESM 3 Kyoto encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of down-regulated DEGs in SeV-infected airway epithelial cells. The x-axis represents the amounts of DEGs, and y-axis represents enriched KEGG pathways. (TIF 210 kb)
42770_2018_21_MOESM4_ESM.tif (227 kb)
ESM 4 Kyoto encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of down-regulated DEGs in SeV-infected macrophages. The x-axis represents the amounts of DEGs, and yaxis represents enriched KEGG pathways. (TIF 226 kb)


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Copyright information

© Sociedade Brasileira de Microbiologia 2018

Authors and Affiliations

  1. 1.School of Basic Medical SciencesHenan UniversityKaifengChina
  2. 2.Department of Physiology and Immunology Programme, Yong Loo Lin School of MedicineNational University of SingaporeSingaporeSingapore

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