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A novel approach to produce glucose from the supernatant obtained upon the dilute acid pre-treatment of rice straw and synergistic action of hydrolytic enzymes producing microbes

  • Manisha Chownk
  • Rajender Singh Sangwan
  • Sudesh Kumar Yadav
Biotechnology and Industrial Microbiology - Research Paper
  • 2 Downloads

Abstract

The present work refers to a process involving the use of dilute nitric acid pretreatment and enzymatic hydrolysis for the transformation of rice straw into simple sugars. Acid pre-treated rice straw was separated into the pulp and supernatant through centrifugation and filtration. The two fractions are then converted into simple sugars by combined action of microbes producing cellulase and laccase enzymes. These microbes were isolated from soil samples which were collected from different locations with varying altitudes, expected to harbour microbes with high-hydrolysing activity. The nitric acid pretreatment was carried out at 30 °C, 200 rpm for 72 h. After 72 h, the culture supernatants were analysed for the presence of glucose with the help of HPLC. The supernatant fraction separated after the acid pre-treated rice straw produced highest amount of glucose (205 mg/g of rice straw) upon subsequent hydrolysis with synergistic action of cellulase and laccase-producing microbes.

Keywords

Rice straw Cellulose Laccase Glucose Biomass transformation 

Notes

Acknowledgements

Authors are thankful to Mr. Umesh Singh, STA, CIAB, for his help in analysis.

Funding information

Authors are thankful to the Department of Biotechnology (DBT), GOI for the financial support to conduct this research.

Supplementary material

42770_2018_13_MOESM1_ESM.docx (119 kb)
ESM 1 (DOCX 118 kb)

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Copyright information

© Sociedade Brasileira de Microbiologia 2018

Authors and Affiliations

  • Manisha Chownk
    • 1
  • Rajender Singh Sangwan
    • 1
  • Sudesh Kumar Yadav
    • 1
  1. 1.Biotechnology and Synthetic BiologyCenter of Innovative and Applied Bioprocessing (CIAB)MohaliIndia

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