A novel approach to produce glucose from the supernatant obtained upon the dilute acid pre-treatment of rice straw and synergistic action of hydrolytic enzymes producing microbes
- 2 Downloads
The present work refers to a process involving the use of dilute nitric acid pretreatment and enzymatic hydrolysis for the transformation of rice straw into simple sugars. Acid pre-treated rice straw was separated into the pulp and supernatant through centrifugation and filtration. The two fractions are then converted into simple sugars by combined action of microbes producing cellulase and laccase enzymes. These microbes were isolated from soil samples which were collected from different locations with varying altitudes, expected to harbour microbes with high-hydrolysing activity. The nitric acid pretreatment was carried out at 30 °C, 200 rpm for 72 h. After 72 h, the culture supernatants were analysed for the presence of glucose with the help of HPLC. The supernatant fraction separated after the acid pre-treated rice straw produced highest amount of glucose (205 mg/g of rice straw) upon subsequent hydrolysis with synergistic action of cellulase and laccase-producing microbes.
KeywordsRice straw Cellulose Laccase Glucose Biomass transformation
Authors are thankful to Mr. Umesh Singh, STA, CIAB, for his help in analysis.
Authors are thankful to the Department of Biotechnology (DBT), GOI for the financial support to conduct this research.
- 1.Sangakkara UR (2005) Rice almanac–source book for the most important economic activity on Earth, eds Maclean JL. Dawe DC. Hardy B. Hettel GP. Wallingford: CABI. The Journal of Agricultural Science 143(1):110Google Scholar
- 2.Adhikari A, Sekhon MK (2014) Export of Basmati rice from India: performance and trade direction. J Agric Dev Policy 24(1):1–13 ISSN: 2322-0457Google Scholar
- 7.Kopke M, Noack S, Dürre P (2011) The Past, present, and future of biofuels – biobutanol as promising alternative, biofuel production-recent developments and prospects. In: Bernards MADS (ed) Biofuel production-recent developments and prospects. InTech, London, pp 451–486. https://doi.org/10.5772/20113 CrossRefGoogle Scholar
- 10.Holladay J, Bozell J, White J, Johnson D (2007) Top value-added chemicals from biomass. DOE Report PNNL-16983. http://www.chembioprocess.pnl.gov/staff/staffinfo.asp.
- 13.Hashem M, Ali EH, Abdel-Basset R (2013) Recycling rice straw into biofuel “ethanol” by Saccharomyces cerevisiae and Pichia guilliermondii. J Agri Sci Technol 15(4):709–721 ISSN:16807073Google Scholar
- 16.Nirmala P, Sindhu A (2011) Production of endoglucanase by optimizing the environmental conditions of Bacillus circulans on submerged fermentation. Int J Appl Eng Res Dindigul 2:472–481 ISSN - 0976-4259Google Scholar
- 17.Sheikhi F, Ardakani MR, Enayatizamir N, Rodriguez-Couto S (2012) The determination of assay for laccase of Bacillus subtilis WPI with two classes of chemical compounds as substrates. Indian J Microbiol 52(4):701–707. https://doi.org/10.1007/s12088-012-0298-3 CrossRefPubMedPubMedCentralGoogle Scholar
- 18.O’Sullivan C, Christopher, Busby BB, Mizrachi IK (2017) Managing Sequence Data. In: Keith JM (ed) Bioinformatics: volume I: data, sequence analysis, and evolution. Springer Publishing, New York, pp 79–106Google Scholar
- 21.Harmsen PFH, Huijgen W, Bermudez L, Bakker R (2010) Literature review of physical and chemical pretreatment processes for lignocellulosic biomass. Wageningen UR Food & Biobased Research, Wageningen, p 1184Google Scholar
- 24.Kshirsagar SD, Waghmare PR, Chandrakant Loni P, Patil SA, Govindwar SP (2015) Dilute acid pretreatment of rice straw, structural characterization and optimization of enzymatic hydrolysis conditions by response surface methodology. RSC Adv 5(58):46525–46533. https://doi.org/10.1039/C5RA04430H CrossRefGoogle Scholar
- 25.Lu X, Zhang Y, Angelidaki I (2009) Optimization of H2SO4-catalyzed hydrothermal pretreatment of rapeseed straw for bioconversion to ethanol: focusing on pretreatment at high solids content. Bioresour Technol 100(12):3048–3053. https://doi.org/10.1016/j.biortech.2009.01.008 CrossRefPubMedGoogle Scholar
- 26.Drapcho CM, Nhuan NP, Walker TH (2008) Biofuels engineering process technology. McGraw-Hill, New YorkGoogle Scholar
- 29.Farone WA, Cuzens JE (1996) Method of producing sugars using strong acid hydrolysis of cellulosic and hemicellulosic materials. U.S. Patent 5562777Google Scholar
- 30.Yoon KP (2010) Method for pretreating biomass to produce bioethanol. U.S patent 2010131829 A1Google Scholar
- 31.Varanasi S, Schall CA, Dadi AP, et al (2011) Biomass pretreatment. U.S. Patent 8030030Google Scholar
- 32.Gordon RE, Haynes WC, Pang CHN (1973) The genus Bacillus. In: O’Leary WM (ed) Practical handbook of microbiology. CRC Press, Boca Raton, pp 109–126Google Scholar
- 35.Poornejad N, Karimi K, Behzad T (2014) Ionic liquid pretreatment of rice straw to enhance saccharification and bioethanol production. J Biomass Biofuel. https://doi.org/10.11159/jbb.2014.002