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Brazilian Journal of Microbiology

, Volume 50, Issue 1, pp 175–183 | Cite as

Characterization of multidrug-resistant Gram-negative bacilli isolated from hospitals effluents: first report of a blaOXA-48-like in Klebsiella oxytoca, Algeria

  • Khadidja Yousfi
  • Abdelaziz Touati
  • Brigitte Lefebvre
  • Philippe Garneau
  • Soumia Brahmi
  • Alima Gharout-Sait
  • Josée Harel
  • Sadjia BekalEmail author
Environmental Microbiology - Research Paper

Abstract

The antibiotic susceptibility profile and antimicrobial resistance determinants were characterized on Gram-negative bacilli (GNB) isolated from Algerian hospital effluents. Among the 94 isolates, Enterobacteriaceae was the predominant family, with Escherichia coli and Klebsiella pneumoniae being the most isolated species. In non-Enterobacteriaceae, Acinetobacter and Aeromonas were the predominant species followed by Pseudomonas, Comamonas, Pasteurella, and Shewanella spp. The majority of the isolates were multidrug-resistant (MDR) and carried different antimicrobial resistance genes including blaCTX-M, blaTEM, blaSHV, blaOXA-48-like, blaOXA-23, blaOXA-51, qnrB, qnrS, tet(A), tet(B), tet(C), dfrA1, aac(3)-IIc (aacC2), aac(6′)-1b, sul1, and sul2. The qacEΔ1-sul1 and intI2 signatures of class 1 and class 2 integrons, respectively, were also detected. Microarray hybridization on MDR E. coli revealed additional resistance genes (aadA1 and aph3strA, tet30, mphA, dfrA12, blacmy2, blaROB1, and cmlA1) and classified the tested strains as commensals, thus highlighting the potential role of humans in antibiotic resistance dissemination. This study is the first report of blaOXA-48-like in Klebsiella oxytoca in Algeria and blaOXA-23 in A. baumannii in Algerian hospital effluents. The presence of these bacteria and resistance genes in hospital effluents represents a serious public health concern since they can be disseminated in the environment and can colonize other hosts.

Keywords

Multidrug resistance Hospital effluents Gram-negative bacilli Algeria 

Notes

Acknowledgments

The authors would like to thank Nancy Cloutier, Isabelle Robillard, Simon Wang, Karine Desjardins, Dominique Paquette, Annie Vezina, and François Robillard for their Lab assistance and Dr. Valentine Usongo for reviewing the manuscript.

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Copyright information

© Sociedade Brasileira de Microbiologia 2018

Authors and Affiliations

  • Khadidja Yousfi
    • 1
    • 2
  • Abdelaziz Touati
    • 1
  • Brigitte Lefebvre
    • 2
  • Philippe Garneau
    • 3
  • Soumia Brahmi
    • 1
  • Alima Gharout-Sait
    • 1
  • Josée Harel
    • 3
  • Sadjia Bekal
    • 2
    • 4
    Email author
  1. 1.Laboratoire d’Écologie Microbienne, FSNVUniversité de BéjaiaBéjaiaAlgeria
  2. 2.Laboratoire de santé publique du QuébecInstitut national de santé publique du QuébecSainte-Anne-de-BellevueCanada
  3. 3.Faculté de Médecine Vétérinaire, Centre de Recherche en Infectiologie PorcineUniversité de MontréalSt-HyacintheCanada
  4. 4.Département de Microbiologie, Infectiologie et ImmunologieUniversité de MontréalMontréalCanada

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