Brazilian Journal of Microbiology

, Volume 50, Issue 1, pp 307–312 | Cite as

Detection of Brucella spp. in dogs at Pantanal wetlands

  • Ana Laura Bello de Oliveira
  • Gabriel Carvalho de Macedo
  • Gracia Maria Soares Rosinha
  • Jhessyca Leal Melgarejo
  • Andreza Gabriela Leão Alves
  • Wanessa Teixeira Gomes Barreto
  • Filipe Martins Santos
  • João Bosco Vilela Campos
  • Heitor Miraglia Herrera
  • Carina Elisei de OliveiraEmail author
Veterinary Microbiology - Research Paper


Canine brucellosis is an infectious disease that produces reproductive disease in both males and females. Although Brucella canis is more common, the infection by Brucella abortus is more frequent in dogs sharing habitats with livestock and wild animals. We decided to investigate the role of dogs in the maintenance of Brucella spp. in the Pantanal wetland. Serum and whole blood samples were collected from 167 dogs. To detect antibodies against B. abortus and B. canis, buffered acidified plate antigen (BAPA) and agar gel immunodiffusion (AGID) tests were performed. To detect Brucella spp., B. abortus and B. canis DNA, PCR was performed using the bcsp31, BruAb2_0168, and BR00953 genes, respectively. To confirm the PCR results, three bcsp31 PCR products were sequenced and compared with sequences deposited in GenBank. The seropositivity rates of 7.8% and 9% were observed for the AGID and BAPA tests, respectively. Positivity rates of 45.5% and 10.8% were observed when testing bcsp31 and BruAb2_0168, respectively, while there was no positivity for BR00953. The sequenced products had 110 base pairs that aligned with 100% identity to B. abortus, B. canis, and B. suis. Considering our results, dogs may be acting as maintenance hosts of Brucella spp. in the Pantanal region.


Brucella spp. Reservoir host Serology Molecular tests Pantanal 



We like to thanks Dra. Lara Borges Keid, who provided the positive controls used in molecular tests.

Compliance with ethical standards

All procedures were performed in accordance with the Ethics Committee for Animal Use of the Universidade Católica Dom Bosco (001/2013).

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Sociedade Brasileira de Microbiologia 2018

Authors and Affiliations

  • Ana Laura Bello de Oliveira
    • 1
  • Gabriel Carvalho de Macedo
    • 1
  • Gracia Maria Soares Rosinha
    • 2
  • Jhessyca Leal Melgarejo
    • 1
  • Andreza Gabriela Leão Alves
    • 1
  • Wanessa Teixeira Gomes Barreto
    • 1
  • Filipe Martins Santos
    • 1
  • João Bosco Vilela Campos
    • 1
  • Heitor Miraglia Herrera
    • 1
  • Carina Elisei de Oliveira
    • 1
    Email author
  1. 1.Universidade Católica Dom Bosco (UCDB)Campo GrandeBrazil
  2. 2.Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) – Gado de CorteCampo GrandeBrazil

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