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Functional Soil Organic Matter Fractions, Microbial Community, and Enzyme Activities in a Mollisol Under 35 Years Manure and Mineral Fertilization

  • Fan Yang
  • Jing TianEmail author
  • Huajun Fang
  • Yang Gao
  • Minggang Xu
  • Yilai Lou
  • Baoku Zhou
  • Yakov Kuzyakov
Research Article
  • 6 Downloads

Abstract

Fertilization is a worldwide practice to maintain and increase crop productivity and improve soil quality in agricultural ecosystems. The interactive mechanisms of long-term fertilization affecting the functional soil organic matter (SOM) fractions, microbial community, and enzyme activities are unclear. We investigated the effects of manure and mineral fertilization on six SOM fractions (non-protected, physically, chemically, biochemically, physical-chemically, and physical-biochemically protected), microbial community structure, and enzyme activities based on a 35-year fertilization experiment. The combined application of manure and mineral fertilizers (NPKM) increased the soil organic carbon (SOC) and total nitrogen (TN) in the biochemically (28.6–43.9%) and physically (108–229%) protected fractions, compared to their content in the unfertilized soil (CK). The total phospholipid fatty acid content, Gram(−) bacteria, and actinomycetes, as well as the activities of α-1,4-glucosidase, β-1,4-N-acetylglucosaminidase, β-1,4-xylosidase, and cellobiohydrolase were highest under NPKM fertilization. The protected SOM fractions (physical, biochemical, physical-chemical, and physical-biochemical) were closely related to microbial community composition (accounting for 67.6% of the variance). Bacteria were sensitive to changes in the physically and biochemically protected fractions, whereas fungi responded more to the changes in the chemically protected fraction. In summary, long-term mineral and organic fertilization has a strong effect on microbial communities and activities through the changes in the functional SOM fractions.

Keywords

Long-term fertilization Soil organic matter fractions Soil aggregation Microbial community composition Enzyme activities 

Notes

Acknowledgements

We thank the Editor and the anonymous reviewers for their valuable comments that helped us to greatly improve the manuscript.

Funding

This study is financially supported by the National Natural Science Foundation of China (Grant No. 41571130041; 31770560) and the Major Program of the National Natural Science Foundation of China (Grant No. 2017YFA0604803). The publication was supported by the Government Program of Competitive Growth of Kazan Federal University and with the support of the “RUDN University program 5-100.”

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Copyright information

© Sociedad Chilena de la Ciencia del Suelo 2019

Authors and Affiliations

  • Fan Yang
    • 1
    • 2
    • 3
  • Jing Tian
    • 1
    • 2
    Email author
  • Huajun Fang
    • 2
  • Yang Gao
    • 2
  • Minggang Xu
    • 4
  • Yilai Lou
    • 5
  • Baoku Zhou
    • 6
  • Yakov Kuzyakov
    • 7
    • 8
    • 9
  1. 1.College of Resources and Environmental Sciences; National Academy of Agriculture Green Development; Key Laboratory of Plant-Soil Interactions, Ministry of Education China Agricultural UniversityBeijingChina
  2. 2.Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research Chinese Academy of SciencesBeijingChina
  3. 3.State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water ConservationChinese Academy of Sciences and Ministry of Water ResourcesYanglingChina
  4. 4.National Engineering Laboratory for Improving Quality of Arable Land, Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijingChina
  5. 5.Institute of Environment and Sustainable Development in AgricultureChinese Academy of Agricultural SciencesBeijingChina
  6. 6.Institute of Soil Fertilizer and Environment ResourcesHeilongjiang Academy of Agricultural SciencesHaerbinChina
  7. 7.Department of Soil Science of Temperate Ecosystems, Department of Agricultural Soil ScienceUniversity of GöttingenGöttingenGermany
  8. 8.Institute of Environmental SciencesKazan Federal UniversityKazanRussia
  9. 9.Agro-Technological InstituteRUDN UniversityMoscowRussia

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