Cereal production has always been an important component of Tunisian agriculture. However, insect pests are considered as a serious biosecurity threat to Tunisian cereals’ production and industry. Several methods such as fumigation have been used in storage. However, their use is controverted due to human and environmental worries. The main objective of this study was to introduce an ecologically safe method for managing the rust–red flour beetle, Tribolium castaneum. The insecticidal activities of Laurus nobilis have been extensively investigated. Therefore, the L. nobilis leaf essential oil chemical composition, and its insecticidal activity against T. castaneum adults were evaluated. Thus, a preliminary screening tests were designed to assess fumigant toxicity of Laurus nobilis essential oil against T. castaneum adults and to determine median effective concentrations (LC50 value). A second experiment was aimed to test the impact of spaces occupation with semolina and the storage period on essential oil effectiveness. T. castaneum adults were treated with the LC50 concentration and mortalities were assessed after 30, and 60 days of storage under 50% and 100% spaces occupied with semolina. Furthermore, the qualitative contamination of semolina by T. castaneum and the effect of essential oil on semolina characteristics and rheological dough properties were elucidated. Results indicated that the oil exhibited strong fumigant potentialities that depend on the exposure period and percentage of space occupation. The LT50 values for T. castaneum ranged from 70.48 h for 50% space occupation with semolina to 43.44 h for 100% space occupation with semolina. Moreover, we investigated the essential oil impact on semolina characteristics (protein, ash, gluten, and moisture) and rheological dough. Results indicated that the oil exhibited strong fumigant potentialities that depend on the exposure period and percentage of space occupation. It was also revealed that essential oil treatment slightly modified semolina characteristics but did not affect rheological properties that still conformed with ISO standards. The results of this study can be interested in further phytochemical and insecticidal activities of L. nobilis essential oil as alternative fumigants for control of stored semolina without negative effect on the quality of semolina.
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Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267
AOAC (1984) Official method of analysis, 14th edn. Association of Official Analytical Chemists, Washington, DC, USA
Arregui L, Quemada M (2008) Strategies to improve nitrogen use efficiency in winter cereal crops under rainfed conditions. Agron J 100:277–284
Arthur FH (2015) Residual efficacy of pyrethrin+ methoprene for control of Tribolium castaneum and Tribolium confusum in a commercial flour mill. J Stored Prod Res 64:42–44
Bachta M (2011) L'Agriculture tunisienne: performances et menaces de non durabilité Institut arabe des chefs d’entreprise
Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils–a review. Food Chem Toxicol 46:446–475
Blandino M, Marinaccio F, Ingegno BL, Vaccino P, Tavella L, Reyneri A (2015) Evaluation of common and durum wheat rheological quality through Mixolab® analysis after field damage by cereal bugs. Field Crop Res 179:95–102
Boxall R (2001) Post-harvest losses to insects—a world overview. Int Biodeter Biodegr 48:137–152
Bruinsma J (2017) World agriculture: towards 2015/2030: an FAO study. Routledge
Chang Y, McLandsborough L, McClements DJ (2015) Fabrication, stability and efficacy of dual-component antimicrobial nanoemulsions: essential oil (thyme oil) and cationic surfactant (lauric arginate). Food Chem 172:298–304
Chaudhry M (2000) Posphine resistance. Pesticide Outlook 11:88–91
Finney DJ (1971) Probit analysis, 3rd ed. Cambridge University Press, London
Foca G, Ulrici A, Corbellini M, Pagani MA, Lucisano M, Franchini GC, Tassi L (2007) Reproducibility of the Italian ISQ method for quality classification of bread wheats: An evaluation by expert assessors. J Sci Food Agric 87:839–846
Gaaloul I, Riabi S, Ellouz Ghorbel R (2011) Implementation of ISO 22000 in cereal food industry “SMID” in Tunisia. Food Control 22:59–66
Hamdi Haouel S, Hedjal-Chebheb M, Kellouche A, Khouja ML, Boudabous A, JMB J (2015) Management of three pests’ population strains from Tunisia and Algeria using Eucalyptus essential oils. Ind Crops Prod 74:551–556
Jarraya A (2003) Principaux Nuisibles des Plantes Cultivées et des Denrées Stockées en Afrique du Nord; leur Biologie, leurs Ennemis Naturels, leurs Dégâts et leur Contrôle. Edition Climat pub edn.,
Karababa E, Ozan AN (1998) Effect of wheat bug (Eurygaster integriceps) damage on quality of a wheat variety grown in Turkey. J Sci Food Agric 77:399–403
Karabörklü S, Ayvaz A, Yilmaz S (2010) Bioactivities of different essential oils against the adults of two stored product insects. Pak J Zool 42(6)
Kivçak B, Mert T (2002) Preliminary evaluation of cytotoxic properties of Laurus nobilis leaf extracts. Fitoterapia 73:242–243
Korunic Z, Rozman V (2008) Fumigacija cineolom in vitro (Fumigation with cineole essential oil in vitro). In: Proceedings of Croatian Seminar DDD and ZUPP, 2008. p 193e203
Latiri K, Lhomme J-P, Annabi M, Setter TL (2010) Wheat production in Tunisia: progress, inter-annual variability and relation to rainfall. Eur J Agron 33:33–42
Mediouni Ben Jemâa J, Tersim N, Toudert KT, Khouja ML (2012) Insecticidal activities of essential oils from leaves of Laurus nobilis L. from Tunisia, Algeria and Morocco, and comparative chemical composition. J Stored Prod Res 48:97–104
Mediouni Ben Jemâa J, Haouel S, Khouja ML (2013) Efficacy of Eucalyptus essential oils fumigant control against Ectomyelois ceratoniae (Lepidoptera: Pyralidae) under various space occupation conditions. J Stored Prod Res 53:67–71
Nicolopoulou-Stamati P, Maipas S, Kotampasi C, Stamatis P, Hens L (2016) Chemical pesticides and human health: the urgent need for a new concept in agriculture. Front Public Health 4:148
Özcan MM (2009) Effect of some essential oils on rheological properties of wheat flour dough. Int J Food Sci Nutr 60:176–181
Ozderen T, Olanca B, Sanal T, Ozay DS, Koksel H (2008) Effects of suni-bug (Eurygaster spp.) damage on semolina properties and spaghetti quality characteristics of durum wheats (Triticum durum L.). J Cereal Sci 48:464–470
Papachristos D, Stamopoulos D (2002) Repellent, toxic and reproduction inhibitory effects of essential oil vapours on Acanthoscelides obtectus (say)(Coleoptera: Bruchidae). J Stored Prod Res 38:117–128
Pimentel MA, Faroni LRDA, da Silva FH, Batista MD, Guedes RN (2010) Spread of phosphine resistance among Brazilian populations of three species of stored product insects. Neotrop Entomol 39:101–107
Rozman V, Korunić Z, Kalinović I (2008) Effect of different quantities of wheat on the effectiveness of the essential oil cineole against stored grain insect pests. In: CAF 2008-Controlled Atmosphere and Fumigation-Green, Safe, Harmony and Development
Saade ME (1996) Adoption and impact of high yielding wheat varieties in northern Tunisia. CIMMYT, economics working paper 96-03. CIMMYT, Mexico
Saeed Mohammad O, Ali W, Tais Al-Hulitan A-M (2012) The effect of infestation by the confused flour beetle (Tribolium Confusum Duv.) on specifications of wheat flour vol 2
Shaaya E, Kostjukovski M, Eilberg J, Sukprakarn C (1997) Plant oils as fumigants and contact insecticides for the control of stored-product insects. J Stored Prod Res 33:7–15
Shakoori AR, Saleem MA, Mantle D (1998) Some macromolecular abnormalities induced by a sublethal dose of Cymbush 10EC in adult beetles of Tribolium castaneum. Pak J Zool (Pakistan) 30:83–90
Singh S, Prakash S (2013) Development of resistance in Tribolium castaneum, Herbst (Coleoptera: Tenebrionidae) towards deltamethrin in laboratory. Int J Sci Res Publ 3:1–4
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Haouel-Hamdi, S., Hamedou, M.B., Bachrouch, O. et al. Susceptibility of Tribolium castaneum to Laurus nobilis essential oil and assessment on semolina quality. Int J Trop Insect Sci (2020). https://doi.org/10.1007/s42690-020-00119-6
- Laurus nobilis
- Space occupation
- Semolina characteristics
- Tribolium castaneum
- Rheological properties