Antifeedant and larvicidal activities of Mentha pulegium on chickpea pod borer Helicoverpa armigera (Lepidoptera: Noctuidae)

  • Rachid BoulamtatEmail author
  • Saadia Lhaloui
  • Abdelhadi Sabraoui
  • Karim El-Fakhouri
  • Ali Oubayoucef
  • Abdelhalim Mesfioui
  • Mustapha El-Bouhssini
Original Research Article


This study investigates the insecticidal effect of the essential oil of Mentha pulegium L. on the second and third instar larvae of Helicoverpa armigera (Hübner). Antifeedant, contact toxicity and systemic toxicity of M. pulegium essential oil were evaluated using four concentrations and an untreated control under laboratory conditions. M. pulegium essential oil showed high level of antifeedant activity at 74.15%, and 61.72% when applied on the second instar larva and third instar larvae, respectively. The second instar larva was also more susceptible to systemic toxicity than the third instar larva LC50 1.80% and 3.80% at 24 h, respectively. Contact toxicity induced with LC50 values of 1.31% and 1.085% after 3 and 6 h on the second instar larva, respectively, and with LC50 values of 2.39% and 2.33% after 3 and 6 h on the third instar larva, respectively. This study showed that there is potential for using M. pulegium essential oil to formulate a biopesticide for the control of H. armigera larvae on chickpea.


Mentha pulegium Helicoverpa armigera Bioinsecticide Chickpea 



The authors are grateful to Dr. Kehel Z, biometrician at ICARDA for his help with statistical analysis of the data.


This work was supported by the International Fund for Agricultural Development, IFAD Grant Number: COFIN-ECG-56-ICARDA.

Compliance with ethical standards

Conflict of interest

The authors declare that they have NO competing financial interest.


  1. Abbot WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267CrossRefGoogle Scholar
  2. Anshul N, Kalra A, Singh D (2014) Biological effect of sweet wormwood, Artemisia annua methanol extracts and essential oil against Helicoverpa armigera hub. (Lepidoptera: Noctuidae). J Entomol Zool Stud 2(6):304–307Google Scholar
  3. Armes NJ, Jadhav DR, DeSouza KR (1996) A survey of insecticide resistance in Helicoverpa armigera in the Indian subcontinent. Bull Entomol Res 86(5):499–514CrossRefGoogle Scholar
  4. Baskar K, Maheswaran R, Kingsley S, Ignacimuthu S (2010) Bioefficacy of Couroupita guianensis (Aubl) against Helicoverpa armigera (hub) (Lepidoptera: Noctuidae) larvae. Span J Agric Res 8(1):135–141CrossRefGoogle Scholar
  5. Ben Jannet H, Skhiri HF, Mighri Z, Simmonds MSJ, Blaney WM (2000) Responses of Spodoptera littoralis larvae to Tunisian plant extracts and to neo-clerodane diterpenoids isolated from Ajuga pseudoiva leaves. Fitoterapia 71:105–112PubMedCrossRefGoogle Scholar
  6. Boughdad A, Elkasimi R, Kharchafi M (2011) Activite Biologique Des Huiles Essentielles De Mentha Sur Callosobruchus Maculatus (F.) (Coleoptera, Bruchidae). Neuvième Conférence Internationale Sur Les Ravageurs En Agriculture Montpellier – 26 et 27 Octobre 2011Google Scholar
  7. Bouyahya A, Et-Touys A, Bakri Y, Talbaui A, Fellah H, Abrini J, Dakka N (2017) Chemical composition of Mentha pulegium and Rosmarinus officinalis essential oils and their antileishmanial, antibacterial and antioxidant activities. Microb Pathog 111:41–49PubMedCrossRefGoogle Scholar
  8. Carvalho FP (2017) Pesticides, environment, and food safety. Food Energy Security 6(2):48–60CrossRefGoogle Scholar
  9. Cetin H, Cinbilgel I, Yanikoglu A, Gokceoglu M (2006) Larvicidal activity of some Labiatae (Lamiaceae) plant extracts from Turkey. Phytother Res J 20(12):1088–1090CrossRefGoogle Scholar
  10. Darabi K, Khajehali J (2017) Bioactivity of essential oils of Mentha species and Cuminum cyminum L. on Anarta trifolii (Hufnagel) (Lepidoptera: Noctuidae). J Essential Oil Bear Plants 20(4):1097–1106CrossRefGoogle Scholar
  11. Derwich E, Benziane Z, Boukir A (2010) GC/MS analysis and antibacterial activity of the essential oil of Mentha pulegium grown in Morocco. Res J Agric Biol Sci 6(3):191–198Google Scholar
  12. Devi VS, Rao PA, Sharma SP, Sharma HC (2014) Interaction of acid exudates in chickpea with biological activity of Bacillus thuringiensis towards Helicoverpa armigera. J Appl Entomol 138(4):289–296CrossRefGoogle Scholar
  13. Durmuşoğlu E, Hatipoğlu A, Gürkan MO, Moores G (2015) Comparison of different bioassay methods fordetermining insecticide resistance in European GrapevineMoth, Lobesia botrana (Denis & Schiffermüller)(Lepidoptera: Tortricidae). Turk J Entomol 39(3):271–276Google Scholar
  14. EPA (2017) Pesticides industry sales and usage 2008 and 2012 market estimates. U.S. Environmental Protection Agency.
  15. Fathipour Y, Sedaratian A (2013) Integrated Management of Helicoverpa armigera in soybean cropping systems. In: El-Shemy HA (ed) Soybean Pest resistance. IntechOpen, London, pp 231–280Google Scholar
  16. Finney DJ (1971) Probit Analysis, 3rd edn. Cambridge University Press, LondonGoogle Scholar
  17. Gopalakrishnan S, Rajendran V, Arumugam S, Sharma HC, Vadlamudi S, Bhimineni RK, Gonzalez SV, Melo TM, Simic N (2016) Insecticidal activity of a novel fatty acid amide derivative from Streptomyces species against Helicoverpa armigera. Nat Prod Res 30(24):2760–2769PubMedCrossRefGoogle Scholar
  18. Grundy DL, Still CC (1985) Inhibition of acetylcholinesterases by pulegone-1,2-epoxide. Pestic Biochem Physiol 23(3):383–388CrossRefGoogle Scholar
  19. Gunderson CA, Samuelian JH, Evans CK, Brattsten LB (1985) Effects of the mint monoterpene, pulegone, on Spodoptera eridania (Lepidoptera: Noctuidae). Environ Entomol 14(5):859–862CrossRefGoogle Scholar
  20. Isman MB (2000) Plant essential oils for pest and disease management. Crop Prot 19(8–10):603–608CrossRefGoogle Scholar
  21. Jacobson M (1989) Botanical pesticides, past present and future. In: Arnason JT (ed) Insecticides of plant origin. Proceeding of the American Chemical Society, Washington, D.C, pp 1–10Google Scholar
  22. Jain S, Jain DK, Balekar N (2012) In-vivo antioxidant activity of ethanolic extract of Mentha pulegium leaf against CCl4 induced toxicity in rats. Asian Pac J Trop Biomed 2(2):S737–S740CrossRefGoogle Scholar
  23. Kamaraj C, Abdul-Rahuman A, Bagavan A (2008) Screening for antifeedant and larvicidal activity of plant extracts against Helicoverpa armigera (Hübner), Sylepta derogata (F.) and Anopheles stephensi (Liston). Parasitol Res 103(6):1361–1368PubMedCrossRefGoogle Scholar
  24. Khanna VG, Kannabiran K (2007) Larvicidal effect of Hemidesmus indicus, Gymnema sylvestre, and Eclipta prostrata against Culex qinquifaciatus mosquito larvae. Afr J Biotechnol 6(3):307–311Google Scholar
  25. Konstantopoulou I, Vassilopoulou L, Mavragani-Tsipidou P, Scouras ZG (1992) Insecticidal effects of essential oils, a study of the effects of essential oils extracted from eleven Greek aromatic plants on Drosophila auraria. Experientia 48(6):616–619PubMedCrossRefGoogle Scholar
  26. Koul O, Shankar JS, Mehta N, Taneja SC, Tripathi AK, Dhar KL (1997) Bioefficacy of crude extracts of Aglaia species (Meliaceae) and some active fractions against lepidopteran larvae. J Appl Entomol 121(1–5):245–248CrossRefGoogle Scholar
  27. Lal OP (1996) An outbreak of pod borer Heliothis armigera (Hubner) on chickpea in eastern Uttar Pradesh (India). J Entomol Res 20:179–181Google Scholar
  28. Lamiri A, Lhaloui S, Benjilali B, Berrada M (2001) Insecticidal effects of essential oils against hessian Fly, Mayetiola destructor (say). Field Crop Res 71(1):9–15CrossRefGoogle Scholar
  29. Lammers JW, MacLeod A (2007) Report of a pest risk analysis: Helicoverpa armigera (Hübner, 1808). [S.l: s.n.], plant protection service (NL) and central science laboratory (UK) joint Pest risk analysis for Helicoverpa armigera.
  30. Liu ZL, Chua SS, Jiangb GH (2011) Toxicity of Schizonpeta multifida essential oil and its constituent compounds towards two grain storage insects. J Sci Food Agric 91(5):905–909PubMedCrossRefGoogle Scholar
  31. López V, Martín S, Gómez-Serranillos MP, Carretero ME, Jäger AK, Calvo MI (2010) Neuroprotective and neurochemical properties of mint extracts. Phytother Res 24(6):869–874PubMedGoogle Scholar
  32. Mahmoudvand M, Abbasipour H, Basij M, Hosseinpour MH, Rastegar F, Nasiri MB (2011) Fumigant toxicity of some essential oils on adults of some stored-product pests. Chile J Agric Res 71(1):83–89CrossRefGoogle Scholar
  33. McCaffery AR (1998) Resistance to insecticides in Heliothine Lepidoptera: a global view. Philos Trans R Soc Lond B 353(1376):1735–1750CrossRefGoogle Scholar
  34. Mishra K, Singh K, Tripathi CPM (2013) Management of pod borer (Helicoverpa armigera) infestation and productivity enhancement of gram crop (Cicer arirtinium) through vermiwash with biopesticides. World J Agric Sci 9(5):401–408Google Scholar
  35. Miyazawa M, Watanabe H, Kameoka H (1997) Inhibition of acetylcholinesterase activity by monoterpenoids with ap-menthane skeleton. J Agr Food Chem 45(3):677–679.CrossRefGoogle Scholar
  36. Mozaffari F, Abbasipour H, Garjan AS, Saboori AR, Mahmoudvand M (2012) Various effects of ethanolic extract of Mentha pulegium on the two-spotted spider mite, Tetranychus urticae (Tetranychidae). Arch Phytopathol Plant Protect 45(11):1347–1355CrossRefGoogle Scholar
  37. Pavela R (2005) Insecticidal activity of some essential oils against larvae of Spodoptera littoralis. Fitoterapia 76(7–8):691–696PubMedCrossRefPubMedCentralGoogle Scholar
  38. Pavela R (2008) Insecticidal properties of several essential oils on the house fly (Musca domestica L.). Phytother Res 22(2):274–278PubMedCrossRefGoogle Scholar
  39. Pavlidou V, Karpouhtsis L, Franzios G, Zambetaki A, Scouras Z, Mavragani-Tsipidou P (2004) Insecticidal and genotoxic effects of essential oils of Greek sage, Salvia fruticosa, and mint, Mentha pulegium, on Drosophila melanogaster and Bactrocera oleae (Diptera: Tephritidae). J Agric Urban Entomol 21(1):39–49Google Scholar
  40. Rahman MM (1993) Infestation and yield loss in chickpea due to pod borer in Bangladesh. Bangladesh J Agric Res 15(2):16–23Google Scholar
  41. Raja N, Jeyasankar A, Jeyakumar VS, Ignacimuthu S (2005) Efficacy of Hyptis suaveolens against Lepidopteran pest. Curr Sci 88(2):220–222Google Scholar
  42. Reed W, Cardona C, Sithanantham S, Lateef SS (1987) Chickpea insect pests and their control. In: Saxena MC, Singh KB (eds) The chickpea. CAB International, Wallingford, pp 283–318Google Scholar
  43. Rim IS, Jee CH (2006) Acaricidal effects of herb essential oils against Dermatophagoides farinae and D. pteronyssinus (Acari: Pyroglyphidae) and qualitative analysis of a herb Mentha pulegium (pennyroyal). Korea J Parasitol 44(2):133–138CrossRefGoogle Scholar
  44. Sabraoui A, El-Bouhssini M, Lhaloui S, Boulamtat R, Bouchelta A (2016) Insecticidal effects of essential oils against chickpea leaf miner, Liriomyza cicerina R. Rev Marocaine Protec des Plant 9:39–46Google Scholar
  45. Saeidi K, Hassanpour B (2014) Efficiency of Mentha piperita L. and Mentha pulegium L. essential oils on nutritional indices of Plodia interpunctella Hübner (Lepidoptera: Pyralidae). J Entomol Acarol Res 46(1):13–17CrossRefGoogle Scholar
  46. Sanap MM, Deshmukh RB (1987) Testing of different insecticides for the control of Heliothis armigera (hub.) on chickpea. Int Chickpea Newslett 17:14Google Scholar
  47. Santana O, Andrésb MF, Sanzc J, Errahmanid N, Lamiri A, González-Colomab A (2014) Valorization of essential oils from Moroccan aromatic plants. Nat Prod Commun 9(8):1109–1114PubMedGoogle Scholar
  48. Sharma HC, Gowda CLL, Stevenson PC, Ridsdill-Smith TJ, Clement SL, Rao GVR, Romies J, Miles M, El-Bouhssini M (2007) Host plant resistance and insect pest management. In: Yadav SS, Redden R, Chen W, Sharma B (eds) Chickpea breeding and management. CAB International, Oxford, pp 520–537CrossRefGoogle Scholar
  49. Srivastava RM, Sehgal VK (2002) Bioefficacy of various insecticides against H. armigera in chickpea. Indian J Entomol 64:17–20Google Scholar

Copyright information

© African Association of Insect Scientists 2019

Authors and Affiliations

  1. 1.Laboratory of EntomologyInternational Center for Agricultural Research in the Dry Areas (ICARDA), Rabat-InstitutesRabatMorocco
  2. 2.Department of Biology, Faculty of Science, Laboratory of Genetics, Neuroendocrinology and BiotechnologyIbn-Tofail UniversityKenitraMorocco

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