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International Journal of Tropical Insect Science

, Volume 39, Issue 4, pp 283–290 | Cite as

Composition and change in the microbiome of Diaphorina citri infected with Candidatus Liberibacter asiaticus in China

  • Xiao-bing Song
  • Ai-tian PengEmail author
  • Jin-feng Ling
  • Yi-ping Cui
  • Bao-ping Cheng
  • Lian-hui ZhangEmail author
Original Research Article
  • 27 Downloads

Abstract

Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is the primary vector of Candidatus Liberibacter asiaticus (Las), which causes the devastating disease Huanglongbing (HLB) in Asian citrus. To examine the effects of pathogens on the diversity and structure of insect-associated bacterial communities, we carried out a molecular analysis using healthy D.citri and Las-infected D.citri as a vector-pathogen model. 16S rRNA Illumina sequencing analysis of D.citri revealed shifts in its microbial diversity in response to pathogen infection. The phylum Proteobacteria predominated in D.citri representing 89.40 and 91.73% of the bacterial communities, while remaining bacterial sequences were mainly assigned to the phyla Actinobacteria, Firmicutes, Bacteroidetes and Acidobacteria. The relative proportions of different groups of bacteria changed significantly after pathogen infection. The relative abundance of bacterial communities between healthy D.citri and Las-infected D.citri were different, and the relative abundance of most dominant bacteria decreased, such as Oscillospira, Lactobacillus and Rubrobacter. However, the relative abundance of Wolbachia increased from 1.81 to 2.14%, and there was no difference in the abundance of Carsonella. In pairwise comparisons, the clone library from healthy D.citri contained greater 16S rRNA gene diversity, as reflected by the higher Shannon index at 2.937 versus 2.756 for the healthy and Las-infected clone libraries, respectively. These data indicated that Las infection has a profound effect on the structure and composition of the bacterial community associated with D.citri.

Keywords

Diaphorina citri Kuwayama Candidatus Liberibacter asiaticus 16S ribosomal RNA Microbiome 

Notes

Funding

This article was supported by the National Key R& D Program of China (2018YFD0201500, 2017YFD0202000), the Public Research and Capacity Building Project of Guangdong (2014B020203003), and the Guangdong Provincial Special Fund of Modern Agricultural Industry Technology Innovation Team (2019KJ108).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© African Association of Insect Scientists 2019

Authors and Affiliations

  1. 1.Plant Protection Research Institute, Guangdong Academy of Agricultural SciencesGuangdong Provincial Key Laboratory of High Technology for Plant ProtectionGuangzhouChina
  2. 2.Guangdong Province Key Laboratory of Microbial Signals and Disease ControlAgricultural College of South China Agricultural UniversityGuangzhouChina

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