Insecticidal activity of botanical oils and other neem-based derivatives against whitefly, Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) on cotton

  • Rishi KumarEmail author
  • Sandhya Kranthi
  • V. S. Nagrare
  • D. Monga
  • Keshav Raj Kranthi
  • Naveen Rao
  • Amarpreet Singh
Original Research Article


The insecticidal and phytotoxicity activities of oils from castor (Ricinus communis), pongamia (Pongamia glabra), sesame (Sesamum indicum), neem (Azadirachta indica) and neem based formulations in combination with a detergent Powder as a surfactants (0.1%) were studied against adults of Bemisia tabaci at their peak activity period during 2016–17 and 2017–18 seasons under laboratory and field conditions. Among the oils, castor oil caused a reduction of whitefly populations by 55.4 & 64.2 and 40.5 & 42.8% under laboratory and field conditions, respectively during both the years, pongamia oil 58.4 & 51.7 and 44.9 & 39.2%, sesame oil 45.5 & 56.4 and 30.8 & 36.9%. Neem oil and its formulated products were reported to be comparatively less effective in reduction of whitefly population after chemical treatments i.e. difenthiauron 50 WP (76.2 & 81.5 and 63.5 & 55.2% under laboratory and field conditions, respectively). Phytotoxicity observed after application of recommended doses of castor, pongamia, sesame and neem oil and its formulated products did not cause any problem on tender cotton leaves. The detergent based solutions were difficult to mix and oily layers appeared after mixing, hence mixing the oils and detergent solutions with sticker Triton-X-100 @1.0 ml/l is more effective and easy to apply on cotton plants.


Insecticidal activity Whitefly Castor oil Pongamia oil Sesame oil Phytotoxicity 



The financial support provided ICAR-CICR, Nagpur is highly acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Abd El-Razik MAA, Zayed GMM (2014) Effectiveness of three plant oils in binary mixtures with pyridalyl, abamectin, spinosad and malathion against Callosobruchus maculatus (F.) adults. Am J Biochem Mol Biol 4:76–85. CrossRefGoogle Scholar
  2. Alemandri V, Vaghi Medina CG, Dumón AD, Argüello Caro EB, Mattio MF, García Medina S, López Lambertini PM, Truol G (2015) Three members of the Bemisia tabaci (Hemiptera: Aleyrodidae) cryptic species complex occur sympatrically in horticultural crops. J Econ Entomol 108(2):405–413. CrossRefGoogle Scholar
  3. Ali SS, Jatoi JA, Ahmed SS, Rizwana H, KhosoAG BFR, Mengal MI, Bugti A, Shahwani SA, Rind MA (2017) Effect of neem products and synthetic insecticides against sucking insect pests of cauliflower under field conditions. Aust J Basic Appl Sci 13:221–225CrossRefGoogle Scholar
  4. Anonymous (2009) Susceptibility test method series (method no.8). Insecticide Resistance Action Committee, Version: 3Google Scholar
  5. Barbosa LF, Marubayashi JM, Marchi BR, Yuki VA, Pavan A, Moriones E, Navas CJ, Krause SR (2014) Indigenous American species of the Bemisia tabaci complex are still widespread in the Americas. Pest Manag Sci 70(10):1440–1445. CrossRefGoogle Scholar
  6. Baskaran V, Narayanasamy P (1995) Traditional Pest control. Caterpillar publications, Mariyappa Nagar, Tamil Nadu, IndiaGoogle Scholar
  7. Beroza M (1954) Pyrethrum synergists in sesame oil. Sesamolin, a potent synergist. J Am Oil Chem Soc 31(7):302–305. CrossRefGoogle Scholar
  8. Beroza M (1956) Insecticide synergists, determination of methylene dioxyphenyl-containing synergists used in analysis of fly sprays. J Agric Food Chem 4(1):53–56. CrossRefGoogle Scholar
  9. Bobade SN, Khyade VB (2012) Detailed study on the properties of Pongamia pinnata (Karanja) for the production of biofuel. Res J Chem Sci 2(7):16–20Google Scholar
  10. CABI (2018) Invasive Species Compendium. Wallingford, UK: CAB International.
  11. Chockalingam S, Vasantha E, Somasundaram P (1983) Efficacy of neem oil extractive (NOE) against Spodoptera litura. Oriental J Zool 3:59–64Google Scholar
  12. Cinthia PS, Patricia VA, Roberto MB, Rodolfo FB, Alfredo JP (2012) Source repellency of hydroethanolic extracts of Ricinus communis (Euphorbiaceae) to Scyphophorus acupunctatus (Coleoptera: Curculionidae) in the laboratory. Fla Entomol 95(3):706–710. 10.1 653/0 24.095.0321CrossRefGoogle Scholar
  13. Copping LG, Duke SO (2007) Natural products that have been used commercially as crop protection agents. Pest Manag Sci 63:524–554. CrossRefGoogle Scholar
  14. Copping LG, Menn JJ (2000) Biopesticides: a review of their action, applications and efficacy. Pest Manag Sci 56:651–676. CrossRefGoogle Scholar
  15. Deletre E, Chandre F, Barkman B, Menut C, Martin T (2016) Naturally occurring bioactive compounds from four repellent essential oils against Bemisia tabaci whiteflies. Pest Manag Sci 72(1):179–189. CrossRefGoogle Scholar
  16. Devakumar C, Saxena VS, Mukerjee SK (1986) Evaluation of neem (Azadirachta indica A. Juss) limnoida and azadirachtin against safflower aphid (Dactynotus carthami HRL). Indian J Entomol 48:467–470Google Scholar
  17. Eagleson C. (1940) Oil synergist for insecticides.
  18. Firdaus S, Vosman B, Hidayati N, Supena EDJ, Visser RG, Van Heusden AW (2013) The Bemisia tabaci species complex: additions from different parts of the world. Insect Sci 20(6):723–733Google Scholar
  19. Gahukar RT (1996) Formulation of neem-based products/pesticides. Pestology 20(9):44–45Google Scholar
  20. Galhiane MS, Rissato SR, Santos LS, Chierice GO, Almeida MV, Fumis T, Chechim I, Sampaio AC (2012) Evaluation of the performance of a castor-oil based formulation in limiting pesticide residues in strawberry crop. 35(2):0100–4042.
  21. Gilbertson RL, Batuman O, Webster CG, Adkins S (2015) Role of the insect super vectors Bemisia tabaci and Frankliniella occidentalis in the emergence and global spread of plant virus. Annu Rev Virol 2(1):67–93. CrossRefGoogle Scholar
  22. Haller HL, La Forge FB, Sullivan WN (1942) Effect of sesamin and related compounds on the insecticidal action of pyrethrum on houseflies. J Econ Entomol 35(2):247–248. CrossRefGoogle Scholar
  23. Hofte M (1999) Recent advances in the biological control of plant pathogens. Special Issues on IPM Pestology:180–193Google Scholar
  24. Ibekwe HN, Ogbu JU, Uwalaka OA, Ngbede SO, Onyegbule UN (2014) Efficacy of plant derived insecticides for control of insect pests of garden egg (Solanum Spp.) in southeastern Nigeria Ibekwe. Int J Sci Technol Res 3(8):2277–8616Google Scholar
  25. Isman MB (2000) Plant essential oils for pest and disease management. Crop Protection 19, 603–608Google Scholar
  26. Isman MB (2006) Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annu Rev Entomol 51:45–66 10.11 46/annurev.ento.51.110104.151146CrossRefGoogle Scholar
  27. Jacobson M (1975) Insecticides from plants: a review of the literature, pp1954–1971.Agricultural handbook, vol 461. U.S Department of Agriculture, Washington, D.C, p 138Google Scholar
  28. Kolli GR, Vijayanand B, Sundararajan R (2013) Evaluation of larvicidal activity of Pongamia pinnata extracts against three mosquito vectors. Asian Pac J Trop Biomed 3(11):853–858. CrossRefGoogle Scholar
  29. Kranthi KR (2015) Cotton statistics and news. Cotton Association of India, Mumbai, India 8:1–4Google Scholar
  30. Mahadav KA, Czosneka S, Ghanimb M (2009) Thermo tolerance and gene expression following heat stress in the whitefly Bemisia tabaci B and Q biotypes. Insect Biochem Mol Biol 39:668–676CrossRefGoogle Scholar
  31. Mann GS, Dhaliwal GS, Dhawan AK (2001) Effect of alternative application of neem products and insecticides on population of Bemisia tabaci and its impact on bollworm damage in upland cotton. Annals Plant Prot Sci 9:22–25Google Scholar
  32. Mastoli BB, Mottolkar PR, Mote UN (1995) Studies on efficacy of bio insecticides against bollworm complex of cotton. Pestology 19(12):27–29Google Scholar
  33. Meera B, Kumar S, Kalidhar SB (2003) A review of the chemistry and biological activity of Pongamia pinnata. J Med Arom Plants Sci 25:441–465Google Scholar
  34. Patel MM, Naik MM, Vyas HN, Patel AF (1986) Evaluation of certain insecticides against whitefly (Trialeurodes ricini Misra) and jassids (Empoasca kerri Pruthi) infesting easter. Indian J Plant Prot 14(1):81–82Google Scholar
  35. Patil SG, Singh R, Kalidhar SB (2004) Effect of methanolic extract and its fractions of Karanj (Pongamia pinnata) seeds on oviposition and egg hatching of Plutella xylostella (Lepidoptera: Yponomeutidae). EntomologiaGeneralis 27:25–23. CrossRefGoogle Scholar
  36. Pavela R (2007) Possibilities of botanical insecticides exploitation in plant protection. Pest Technology 1(1):47–52Google Scholar
  37. Pavela R, Herda G (2007) Repellent effects of pongam oil on settlement and oviposition of the common greenhouse whitefly Trialeurodes vaporariorum on chrysanthemum. Insect Sci 14:219–224. CrossRefGoogle Scholar
  38. Pavela R, Herda G (2007a) Effect of pongam oil on adults of the greenhouse whitefly Trialeurodes vaporariorum (Homoptera: Trialeurodidae). Entomologia Generalis 30:193–201CrossRefGoogle Scholar
  39. Rampadarath S, Puchooa D, Jeewon R (2016) Jatropha curcas L: phytochemical, antimicrobial and larvicidal properties. Asian Pac J Trop Biomed 6(10):858–865. CrossRefGoogle Scholar
  40. Salem N, Bachrouch O, Sriti J, Msaada K, Khammassi S, Hammami M, Selmi S, Boushih E, Koorani S, Abderraba M, Marzouk B, Limam F, Mediouni J, Jemaa B (2018) Fumigant and repellent potentials of Ricinus communis and Mentha pulegium essential oils against Tribolium castaneum and Lasioderma serricorne. Internal Journal of Food Properties 21(1):2899–2913. Google Scholar
  41. Simanton WA (1949) Sesame extracts synergized insecticides IOP Publishing New York State Integrated Pest Management Program. Accessed 26 June 2007
  42. Singh G, Upadhyay RK (1993) Essential oil: a potent source of natural pesticides. J Sci Ind Res 52:676–683Google Scholar
  43. Sridhar S, Vijayalakshmi K (2002). Neem: A User's Manual. CIKS. Chennai.43Google Scholar
  44. Stepanycheva EA, Petrova MO, Chermenskaya TD, Pavela R (2014) Prospects for the use of Pongamia pinnata oil-based products against the Green Peach Aphid (Sulzer) (Hemiptera: Aphididae). Psyche: A Journal of Entomology 1–5.
  45. Sureshgouda and Singh R (2004) Antifeedant activity of Pongamia pinnata methanol seed extracts and fractions against Plutella xylostella. J. Med. Arom.Plant Sc. 26(1), 39–43Google Scholar
  46. Swathi S, Muruananthan G, Ghosh SK (2010) Oviposition deterrent activity from the ethanolic extract of Pongamia pinnata, Coleus forskohlii, and Datura stramoniumleaves against Aedes aegypti and Culex quinquefaciatus. Pharmacogn Mag 6(24):320–322. CrossRefGoogle Scholar
  47. Tracy RL (1958) Process for extracting pyrethrin synergists from sesame oil. IOP Publishing New York State Integrated Pest Management Program. Accessed 26 June 2007
  48. Vasudevan P, Madan N, Sharma S (1989) Ovicidal property of castor. Pesticides 2:36–39Google Scholar

Copyright information

© African Association of Insect Scientists 2019

Authors and Affiliations

  • Rishi Kumar
    • 1
    Email author
  • Sandhya Kranthi
    • 2
  • V. S. Nagrare
    • 2
  • D. Monga
    • 1
  • Keshav Raj Kranthi
    • 3
  • Naveen Rao
    • 1
  • Amarpreet Singh
    • 1
  1. 1.ICAR-Central Institute for Cotton Research, Regional StationSirsaIndia
  2. 2.ICAR-Central Institute for Cotton ResearchNagpurIndia
  3. 3.Technical Information Section, ICACWashingtonUSA

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