PCR based genetic marker for the detection of Leptospira interrogans causing leptospirosis
- 2 Downloads
Leptospirosis caused by Leptospira interrogans is a major health concern across the globe. It presents a wide range of clinical manifestations viz. fever, headache, nausea, vomiting and abdominal pain. If left untreated, it can lead to fatal complications. The current methods used for leptospirosis diagnosis are based on the detection of L. interrogans. The methods used for routine leptospirosis diagnosis and the agglutination test which is the gold standard method are time consuming and have shortcomings. Molecular methods such as polymerase chain reaction (PCR) are considered quick, and better in terms of sensitivity and specificity. Keeping in view the importance of PCR in disease diagnosis and pathogen detection the present study is aimed at PCR based detection of Leptospira interrogans using LipL 32 gene specific primers. The primers used in this study amplified partial region of the LipL32 gene under specific conditions. The DNA amplicon of 392 bp confirmed partial amplification of LipL32 gene and showed no homology with any other organism when checked by BLAST. This method seems to be quick, specific and more sensitive as compared to other conventional methods.
KeywordsLeptospira interrogans LipL 32 Leptospirosis Polymerase chain reaction
Polymerase chain reaction
The authors are thankful to Prof. P.K. Khosla, Shoolini University, Solan (H.P) and Dr. Sunil Sethi, PGIMER, Chandigarh for providing facilities to carry out the research work.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- Ahmed SN, Shah S, Ahmad FMH (2005) Laboratory diagnosis of leptospirosis. J Postgrad Med 51:195–200Google Scholar
- Ahmed SA, Sandai DA, Musa S, Hoe CH, Riadzi M, Lau KL et al (2012) Rapid diagnosis of leptospirosis by multiplex PCR. Malay J Med Sci 19:9Google Scholar
- Atzingen MV, Barbosa AS, Brito DE (2008) Lsa21, a novel leptospiral protein binding adhesive matrix molecules and present during human infection. BMC Microbiol 2180:8–70Google Scholar
- Marie L, Lisa L, Andrew ES (2000) The role of DNA amplification technology in the diagnosis of infectious diseases. CMAJ 63:301–309Google Scholar
- Michael AG, Stanford TS (2004) Rapid diagnosis of pharyngitis caused by group A streptococci. Clin Microbiol Rev 17:571580Google Scholar
- Waitkins SA (1987) Leptospirosis. In: Manson-Bhor PEC, Bell DR (eds) Manson’s tropical diseases, 19th edn. Bailliére Tindall, London, pp 657–665Google Scholar