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Vegetos

, Volume 32, Issue 4, pp 583–592 | Cite as

Nitric oxide improved salt stress tolerance by osmolyte accumulation and activation of antioxidant defense system in seedling of B. juncea (L.) Czern

  • Khushboo Khator
  • Gyan Singh ShekhawatEmail author
Research Articles
  • 1 Downloads

Abstract

The present manuscript elucidates the cytoprotective role of nitric oxide and its relation with ROS and antioxidants in minimizing the effect of salinity in B. juncea (Indian mustard) which is an important salt tolerant crop of arid and semi arid region. NO alleviates the adverse effect of salt on growth, biomass, relative water content and photosynthesis. At 50 mM NaCl concentration, rapid accumulation of NO was observed that is 2.02 folds higher in roots respectively while in leaves and roots insignificant changes were observed. At higher concentration of salt stress (70–100 mM NaCl), considerable rise in H2O2 content and membrane damage were documented which restrained by the application of 100 µM SNP. NO mitigates the salt stress by regulating the activities of nitrate reductase and other antioxidants. Reduction in NO content was observed in independent treatment of NaCl and SNP in comparison to combined treatment of SNP + NaCl (100 µM SNP + 50 mM NaCl). The different antioxidant enzymes show disparate behaviour with signalling molecule NO. The activity of catalase, guaiacol peroxidase and nitrate reductase were upregulated whereas ascorbate peroxidase was downregulated with NO. The elevated activities of antioxidant and NO accumulation might be responsible for detoxifying the detrimental effect of salinity in B. juncea. The current investigation emphasizes on the potential role of NO in salt stress tolerance by regulating antioxidative defense metabolism, cellular homeostasis and detoxification mechanism in seedlings of B. juncea.

Keywords

Nitric oxide Salt stress Stress tolerance Antioxidant Brassica juncea 

Abbreviations

NO

Nitric oxide

SNP

Sodium nitroprusside

NR

Nitrate reductase

Chl

Chlorophyll

NaCl

Sodium chloride

TBA

Thiobarbituric acid

TCA

Trichloroacetic acid

MDA

Malondialdehyde

BSA

Bovine serum albumin

HgCl2

Mercuric chloride

EDTA

Ethylene diamine tetraacetic acid

CAT

Catalase

APX

Ascorbate peroxidase

GPOX

Guaiacol peroxidase

H2O2

Hydrogen peroxide

O2

Superoxide radical

ROS

Reactive oxygen species

NADH

Nicotinamide adenine dinucleotide hydride

Notes

Acknowledgements

Author gratefully acknowledges the University Grant Commission, New Delhi for providing financial assistance in the form of Centre for Advanced Study.

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Copyright information

© Society for Plant Research 2019

Authors and Affiliations

  1. 1.Department of BotanyJai Narain Vyas UniversityJodhpurIndia

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